Application of alpelisib compound in preparation of anti-ev71 virus medicine

A compound and drug technology, applied in the application field of preparing anti-EV71 virus drugs, achieves great clinical application prospects, inhibits replication and proliferation, and enhances the effect of survival rate

Active Publication Date: 2022-04-01
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, BYL719 is rarely reported for antiviral therapy

Method used

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  • Application of alpelisib compound in preparation of anti-ev71 virus medicine
  • Application of alpelisib compound in preparation of anti-ev71 virus medicine
  • Application of alpelisib compound in preparation of anti-ev71 virus medicine

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Effect test

Embodiment 1

[0022] The present invention has carried out anti-EV71 activity research experiments on the above-mentioned compounds, and the experimental conditions are as follows: In the following, if not specified, the materials and operation methods used in the present invention are well known in the art.

[0023] 1. Test content:

[0024] Anti-EV71 activity analysis of compounds: In this invention, the anti-EV71 activity of BYL719 will be evaluated by combining cytopathic effect analysis and MTT assay cell survival rate detection method.

[0025] 2. Test method:

[0026] 2.1.1 Toxicity of compounds to host RD cells

[0027] Plate RD cells into 96-well plates at 37 °C, 5% CO 2 After the monolayer was grown in the incubator, the cell culture solution was discarded, and the cell maintenance solution containing different concentrations of the test compound was added to continue the culture. After 48 hours, the cytotoxicity was visually observed under the microscope and recorded respective...

Embodiment 2

[0039] The present invention has carried out an in-depth study on the anti-EV71 activity of BYL719, and implemented the inhibitory effect test of the compound on the production of EV71 progeny virus. The test conditions are as follows:

[0040] 1. Test content

[0041] After EV71 infected RD cells, the inhibitory effect of the compound on the production of EV71 progeny virus was detected.

[0042] 2. Test method

[0043] RD cells in the logarithmic growth phase were plated in 24-well plates, and 100TCID after the monolayer was overgrown 50 EV71 infected cells, incubated at 37°C for 1.5h, removed the virus solution, washed three times with PBS, and added cell maintenance solution containing 12.5μM concentration. After 48 hours, the cells and supernatant were collected and lysed by freezing and thawing three times at -20°C and 37°C. TCID 50 Methods The titer of EV71 virus was determined.

[0044] 3. Test results

[0045] Such as image 3 As shown, compared with the virus ...

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Abstract

The invention discloses the application of an Alpelisib compound in the preparation of anti-EV71 virus medicine. Through the research experiment of anti-EV71 activity of BYL719, the compound BYL719 inhibits the cytopathic effect (CPE) produced by EV71 on the host cell RD, enhances the cell survival rate, reduces the production of progeny virus, and can be used in the preparation of anti-EV71 virus drugs.

Description

technical field [0001] The technical field of antiviral drugs of the present invention relates to the application of an Alpelisib compound in the preparation of anti-EV71 virus drugs. Background technique [0002] Enterovirus 71 (EV71) is a member of the genus Enterovirus in the Picornaviridae family, and is one of the most important pathogens causing hand, foot and mouth disease in infants, sometimes accompanied by severe central nervous system complications. Including aseptic meningitis, encephalitis, poliomyelitis-like paralysis, nervous cardiopulmonary failure, etc., and even death. Since the first report in 1974, EV71 infectious disease has broken out and spread many times around the world, especially in the Asia-Pacific region, especially in my country. In view of the great harm that the spread of hand, foot and mouth disease has brought to the lives and health of our people, the Chinese government has listed hand, foot and mouth disease as a Class C infectious diseas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4439A61P31/14
CPCA61K31/4439A61P31/14
Inventor 魏艳红胡康洪刘会会胡达李涵洛
Owner HUBEI UNIV OF TECH
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