Lactobacillus plantarum LR002 and application thereof
A technology of Lactobacillus plantarum and culture, applied in the field of microorganisms, can solve the problem of not finding Lactobacillus plantarum, etc., and achieve the effect of good acid resistance and good bacteriostatic function
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Embodiment 1
[0031] Isolation and identification of embodiment 1 bacterial strain
[0032] The stems and leaves of quinoa in Lintan County, Gannan Tibetan Autonomous Prefecture were weighed, placed on MRS solid medium and covered with a layer of water agar for separation, and incubated at a constant temperature of 37°C for 48h. Get the colonies, see the results of the colonies figure 1 .
[0033] Colonies were picked, streaked continuously, separated and purified, and finally a single colony was selected. After microscopic examination confirmed that it was a pure species, it was inoculated into slant medium and cultured for 24 hours, and then stored in a refrigerator at 4°C for later use. Gram staining and physiological and biochemical identification were carried out on the strains identified as pure species. For the results of Gram staining, see figure 2 , the physiological and biochemical results are shown in Table 1.
[0034] Table 1 Physiological and biochemical identification resu...
Embodiment 2
[0045] Example 2 bacterial strain acid resistance experiment
[0046] After the strain is activated, the cell concentration is 10 7 cfu / ml bacterial suspension, put 4% inoculum amount into MRS liquid medium with pH values of 1, 2, and 3 respectively, culture it statically at 37°C, and absorb bacteria from it at 0, 1, 2, and 3 hours respectively The suspension was serially diluted, spread on the corresponding solid medium, and cultured upside down in a constant temperature incubator at 37°C for 48 hours, and the plates with 30-300 colonies were selected for counting, and the average value was calculated in 3 parallels. To obtain the tolerance of the strain to acid, the results are shown in Figure 5 .
[0047] Figure 5 The results showed that the strain had good acid resistance, and the growth phenomenon occurred under the condition of pH3.0, and the survival rate of the strain decreased with the prolongation of the culture time at pH2.0 and pH1.0.
Embodiment 3
[0048] Example 3 bacterial strain bile salt resistance experiment
[0049] After the strain is activated, the cell concentration is 10 8 The cfu / ml bacterial suspension was inserted into the MRS liquid medium with bile salt concentrations of 0.3, 1.5, and 3 g / L respectively according to the inoculation amount of 4%, and cultured at 37°C, and the bacteria were drawn from it at 0 and 3 hours respectively. The suspension was serially diluted, spread on the corresponding solid medium, and cultured upside down in a constant temperature incubator at 37°C for 48 hours, and the plates with 30-300 colonies were selected for counting, and the average value was calculated in 3 parallels. Calculate the tolerance of the strain to bile salts, see the tolerance results Figure 6 .
[0050] The calculation formula of the tolerance of described bacterial strain to bile salt is: bile salt tolerance (%)=N t / N 0 ×100%.
[0051] In the formula: N t : The number of viable bacteria in the 3h ...
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