Imaging flow cytometry adhesion counting and activity detection method and device based on bidirectional background difference process

Abstract

The invention provides an imaging flow cytometry adhesion counting and activity detection method and device based on a bidirectional background difference process. The method comprises the following steps: acquiring a bright field image and a cell fluorescence image of a to-be-detected sample and a background image during empty detection, and carrying out binarization processing on the cell fluorescence image; extracting a target contour difference image and a target center bright spot difference image by using a bidirectional background difference method, and carrying out fusion and binarization processing on the two difference images to determine the position of a moving target; performing morphological closed operation on the binarized image, and then performing morphological open operation; and traversing the binary image matrix to realize quantity calculation of the cells to be detected and identification and classification of types of the cells to be detected. According to the technical scheme, the problems of time and labor consumption, incomplete functions, low flux, high price and the like in the prior art are solved. Counting and activity judgment of multiple cells such as microalgae cells, 293T lymphocytes and mouse cells can be achieved, the application range is wide, and identification indexes are stable.

Description

technical field [0001] The present invention relates to the technical field of imaging flow cytometry detection and counting, in particular to a method and device for imaging flow cytometry adhesion counting and activity detection based on a two-way background difference method. Background technique [0002] Single cell counting and imaging are key technologies in medicine, environmental science, biology, and other related fields. They have important application value in disease prediction, disease diagnosis, cell culture, and environmental monitoring. Cell activity detection is also a part of cell biology experiments. It is an important indicator and is the basis for evaluating cell culture, cytotoxicity and physiological research. In the medical field, cell counting has very important clinical significance, such as lymphocytes, adult lymphocytes account for about 1 / 4 of white blood cells, and are important immune cells in the human body. Increased lymphocyte counts are see...

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Problems solved by technology

[0006] According to the above, the existing detection methods have technical problems such as time-consuming, labor-intensive, low-throughput, expensive, etc., and provide an imaging flow cytometry adhesion counting and activity detection method and device based on the two-way background difference method

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