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Method for determining colony count of pathogenic microbes in dehydrated garlic product

A technology of pathogenic microorganisms and the total number of colonies, which is applied in the field of microbial detection, can solve the problems that are not suitable for the safety and sanitation detection of dehydrated garlic products, and achieve the effect of rapid detection and simple steps

Inactive Publication Date: 2013-12-18
安徽出入境检验检疫局检验检疫技术中心
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

So why the current GB4789 series standards are not suitable for the safety and sanitation testing of dehydrated garlic products? Because garlic contains a natural plant antibacterial agent - allicin, allicin is a general term for sulfur compounds in garlic cloves and garlic essential oil, which has different degrees of antibacterial (inhibitory) effects, and is known as a natural fungicide. It is reported that its ability to kill bacteria is almost 100 times that of penicillin

Method used

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  • Method for determining colony count of pathogenic microbes in dehydrated garlic product

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1 Materials and instruments

[0015] 1.1 Materials

[0016] Salmonella species ( salmonella ) is provided by Beijing Lanbo Rui Company, various culture media are provided by Beijing Luqiao Company, Ciba series food-grade polyacrylamide cationic resin, and the samples are from Anhui Donggan Food Co., Ltd. for inspection samples;

[0017] 1.2 Instruments

[0018] Desktop high-speed refrigerated centrifuge (BECKMAN 64R), vertical high-speed refrigerated centrifuge (BECKMAN J30I), micro-sampler: 0.5 ul~10ul, 10 ul~100ul, autoclave.

[0019] 2 Bacteria removal rate experiment

[0020] Weigh 25g of dehydrated garlic product sample (sterilized by irradiation), manually add one McFarland unit (3.5×10 8 Individuals / g) Salmonella strains, add 225mL sterilized diluent to homogenize, filter with a homogeneous bag with a filter membrane, the filter membrane has a pore size of 330μm and a diameter of 50mm; absorb the clear liquid, and divide 1.5mL clear liquid into two 2m...

Embodiment 2

[0026] 1 material and instrument are identical with embodiment 1

[0027] 2 Comparison of the high-speed centrifugation method for the determination of the total number of colonies and the traditional method

[0028] Take 25g of garlic powder sample, add 225mL of sterilized diluent, homogenize and filter through a homogeneous bag with a filter membrane, absorb the clear liquid, and divide 1.5mL into two 2mL centrifuge tubes; Add a small amount of polyacrylamide cationic resin into the centrifuge tube as a coagulant aid, and the concentration of the coagulant aid in the supernatant is 0.1ppm; centrifuge for 40 minutes at a temperature of 4°C and a speed of 20,000 rpm, remove the supernatant, and remove the supernatant in the sediment Add 1.5 mL of sterile distilled water and mix well, measure the amount of bacteria in the sediment according to the national standard GB 4789.2-2010, and use the standard method for comparison; other conditions are the same as in Example 1.

[002...

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Abstract

The invention discloses a method for determining colony count of pathogenic microbes in dehydrated garlic product. The method comprises taking garlic powder sample, adding sterilized diluent, homogenizing, filtering with homogenizing bag equipped with filter membrane, sucking filtrate, transferring into two centrifuge tubes, adding polyacrylamide cationic resin as flocculant, centrifuging, removing supernatant, adding sterile distilled water into precipitate, mixing, and counting bacteria in the precipitate according to national standard GB 4789.2-2010. The invention has fast detection, simple procedure, and precisely detects colony count in bacteria inhibitive samples such as dehydrated garlic in 3 hr.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a method for separating and measuring the total number of pathogenic microorganism colonies in dehydrated garlic products by using a high-speed centrifugation method. Background technique [0002] Food hygiene and safety issues are closely related to everyone's health, and have always been concerned by governments and mainstream media around the world. In recent years, as people pay more and more attention to food safety, especially under the background of frequent food safety accidents in my country in recent years, and food quality and safety incidents related to Chinese export products are often reported in the newspapers, importing countries Food testing from China is getting stricter. my country is the world's largest producer and exporter of garlic (Allium sativum L.) and its products, and has become my country's first foreign currency export veg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/06
Inventor 余晓峰郑海松宗凯孙娟娟陈雪娇李云飞姚剑严轶
Owner 安徽出入境检验检疫局检验检疫技术中心
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