Products and uses for the diagnosis of lung cancer
A product and technology of lung cancer, applied in the field of disease diagnosis, can solve the problems of difficult early detection and lack of specific clinical manifestations of lung cancer
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[0069] Example Gene markers associated with lung cancer diagnosis
[0070] 1. Data download
[0071] Lung adenocarcinomas were searched using the Gene Expression Omnibus database GEO (https: / / www.ncbi.nlm.nih.gov / geo / ) and The Cancer Genome Atlas database TCGA (https: / / cancergenome.nih.gov / ) expression profile data. Samples with missing clinical information were excluded, and samples with matched cancer and adjacent tissues were included. TCGA included 59 adjacent samples and 500 lung adenocarcinoma samples; GEO included 20 adjacent samples and 226 lung adenocarcinoma samples. .
[0072] 2. Data processing and analysis
[0073] For datasets in TCGA, RNA-sequencing data of gene expression (FPKM values) and clinical information were downloaded from UCSC Xena (https: / / gdc.xenahubs.net), and FPKM values were converted to kilobases per million (TPM) transcript of the value.
[0074] For the GEO database download the raw "CEL" file of GSE31210, background adjustment and quant...
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