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Antibody diluent for immunohistochemical detection and preparation method and application thereof

An immunohistochemistry, antibody dilution technology, applied in chemical instruments and methods, immunoglobulins, biological testing, etc., can solve the problems of unfavorable safety experiments and safety management, poor storage stability of antibodies, and high toxicity of sodium azide, Achieve broad-spectrum bacteriostasis, maintain activity and stability, and enhance storage stability

Active Publication Date: 2021-08-10
图凌(杭州)生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PBS or PBST is used as the antibody diluent to reduce the impact of pH changes on the experimental results through its buffering effect, but this method has problems such as low detection intensity, poor antibody storage stability, and low detection sensitivity
There is a method of adding sodium azide preservative to enhance the storage stability of antibodies, but sodium azide is highly toxic and explosive, which is not conducive to safety experiments and safety management

Method used

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  • Antibody diluent for immunohistochemical detection and preparation method and application thereof
  • Antibody diluent for immunohistochemical detection and preparation method and application thereof
  • Antibody diluent for immunohistochemical detection and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation of Example 1 Antibody Diluent #1-#5

[0048] This embodiment provides 5 kinds of antibody dilutions #1-#5 with Tris-HCl buffer as the main component, all including bovine serum albumin (BSA), bis-imidazolidinyl urea antibacterial agent and Tween, the specific formula is as follows Table 1 shows.

[0049] Table 1 Antibody Diluent #1-#5 Recipe

[0050]

[0051] The preparation method is as follows:

[0052] Accurately weigh or measure BSA, bis-imidazolidinyl urea, and Tween in a clean and sterilized container, add Tris-HCl buffer solution (pH7.4), stir and mix well, then filter through a 0.22μm filter membrane and store spare.

Embodiment 2

[0053] Preparation of Example 2 Antibody Diluent #6

[0054] This example provides an antibody diluent #6 with PBS buffer as the main component, including bovine serum albumin (BSA), bis-imidazolidinyl urea antibacterial agent and Tween. The specific formula is shown in Table 2.

[0055] Table 2 Antibody Diluent #6 Recipe

[0056] components content 1×PBS (mL) 999.5 Tween-20(mL) 0.5 Diazolidinyl urea (g) 2 BSA (g) 2.5

[0057] Its preparation method is as follows:

[0058] Accurately weigh or measure BSA, bis-imidazolidinyl urea, and Tween in a clean and sterilized container, add 1×PBS buffer solution (pH7.4), stir well, and then filter through a 0.22μm filter membrane and store spare.

[0059] Wherein, the preparation method of 1×PBS buffer solution is as follows:

[0060] Weigh 8.0g of sodium chloride, 0.2g of potassium chloride, 2.9g of disodium hydrogen phosphate dodecahydrate and 0.2g of potassium dihydrogen phosphate, dissolve ...

Embodiment 3

[0061] The preparation of embodiment 3 antibody diluent #7-#9

[0062] This embodiment provides 3 kinds of antibody diluents #7-#9 with Tris-HCl buffer as the main component, all including bovine serum albumin (BSA), bis-imidazolidinyl urea antibacterial agent and Tween, the specific formula is as follows Table 3 shows.

[0063] Table 3 Antibody Diluent #7-#9 Recipe

[0064]

[0065] The preparation method is as follows:

[0066] Accurately weigh or measure BSA, bis-imidazolidinyl urea, and Tween in a clean and sterilized container, add Tris-HCl buffer solution (pH7.4), stir and mix well, then filter through a 0.22μm filter membrane and store spare.

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Abstract

The invention discloses an antibody diluent for immunohistochemical detection, and belongs to the technical field of antibody detection. The antibody diluent is a buffer solution containing bovine serum albumin with the mass fraction of 0.1%-1%, diazolidinyl urea with the mass fraction of 0.1%-0.5% and Tween with the volume fraction of 0.01%-0.1%. The antibody diluent provided by the invention can improve the strength, sensitivity and stability of immunohistochemical detection, can be used as a diluent of an antibody product, is used for production of an immunohistochemical antibody reagent, and can improve the storage stability of the immunohistochemical antibody reagent.

Description

technical field [0001] The invention belongs to the technical field of antibodies, and in particular relates to an antibody diluent for immunohistochemical detection and its preparation method and application. Background technique [0002] Immunohistochemistry (abbreviated as immunohistochemistry) is a detection method that uses the principle of specific binding of antigen and antibody to make the substrate of the labeled antibody develop color through chemical reaction to determine whether there is target antigen and its expression in the tissue. It combines the specificity of the immune response and the visibility of histochemistry, and uses the imaging and magnification of microscopes (including fluorescence microscopes and electron microscopes) to detect various antigenic substances at the cellular and subcellular levels, and to locate them. Qualitative and quantitative research. [0003] The formulation of antibody diluent plays a key role in the detection strength of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K16/00
CPCG01N33/53C07K16/00
Inventor 陶娜娜章月凯
Owner 图凌(杭州)生物医药有限公司
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