Method for identifying animal-derived components in salmonidae fish product based on high-throughput sequencing

A high-throughput technology for fish products, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of difficult separation of organisms, complex components, and difficult detection, etc., to overcome the low efficiency or inability of PCR amplification Effects of amplification, process simplification and cost reduction

Inactive Publication Date: 2021-08-24
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method usually only detects one or a few species at a time, and cannot realize the simultaneous identification of multiple source components in unknown samples.
When the number of samples is large, it takes a lot of material and manpower
In addition, when the sample to be tested is a mixture or an unknown sample, the separation of organisms is more difficult. These existing animal product adulteration identification technologies are far behind in terms of the range of products involved and the applicability of the method. unable to meet real needs
Especially with the development of modern industry, there are more and more types of salmonid fish products, the processing is more and more refined, and the ingredients are more and more complex. The original characteristics of the species have disappeared, resulting in adulteration and concealment, which is difficult to detect.

Method used

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  • Method for identifying animal-derived components in salmonidae fish product based on high-throughput sequencing
  • Method for identifying animal-derived components in salmonidae fish product based on high-throughput sequencing
  • Method for identifying animal-derived components in salmonidae fish product based on high-throughput sequencing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this example, the accuracy and sensitivity of the high-throughput sequencing technology in the identification of mixed species are evaluated through the following tests.

[0027] 1. Preparation of Mixed Salmonidae Samples

[0028] Each sample was dehydrated and dried in a freeze dryer and then ground into powder. Based on the sample mass, 9 fish samples of known origin and species were collected according to different proportions and methods (mixing of fish species and mixing of fish, livestock and poultry) Gradient blending was performed to prepare mixed samples (Table 1). At the same time, a minimum sample mixing ratio of 1% was set to evaluate the accuracy, detection throughput and sensitivity of high-throughput sequencing technology in terms of species characterization.

[0029] Table 1 Preparation scheme of mixed samples of salmonids

[0030]

[0031] 2. Sample Genomic DNA Extraction

[0032] The improved CTAB method was used to extract the genomic DNA of ...

Embodiment 2

[0046] In this example, the animal-derived species components in commercially available salmonid fish products were analyzed through the following tests.

[0047] 1. Experimental method

[0048] A total of 32 commercially available salmonid fish foods were collected from retailers, supermarkets and e-commerce platforms, basically covering the types of salmonid fish foods currently on the market. DNA was extracted, and the remainder was consistent with Example 1.

[0049] 2. Analysis of results

[0050] Twenty-nine (90.6%) of the 32 commercially available salmonids food products used "salmon" as the food name. The identification results found that the main species involved include Atlantic salmon ( S. salar ) and humpback salmon ( O. gorbuscha ); a small number of samples (7, 8) are marked with the common name of the species in addition to "salmon". Refer to the relevant introductions about salmonids in the books "Species and Distribution of Marine Life in China" and "Fis...

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PUM

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Abstract

The invention relates to the technical field of food quality safety detection, and particularly discloses a method for identifying animal-derived components in salmonidae fish and salmonidae fish products based on a high-throughput sequencing technology. The method comprises the following steps: extracting genome DNA, performing PCR amplification on the extracted genome DNA by using a 16S rRNA universal primer pair with a base tag and an amplification length of 255 bp, constructing an amplicon sequencing library, performing high-throughput sequencing, performing bioinformatics data analysis, performing result evaluation and the like. According to the method, various animal-derived components in salmonidae fish and salmonidae fish products can be identified through one-time detection, and the detection sensitivity is as low as 1%.

Description

technical field [0001] The invention belongs to the technical field of food quality and safety detection. Specifically, the invention relates to a method for identifying animal-derived components in salmonid fish products based on high-throughput sequencing. Background technique [0002] Salmonidae (Salmonidae) belongs to the chordata (Chordata) Salmoniformes (Salmoniformes), including 3 subfamilies (Salmoninae, Whitefish subfamily and Grayling subfamily), 7 genera (Salmoninae , Char, Salmon, Salmon, Salmon, Whitefish and Grayling), a total of about 220 species of fish. Salmonidae is a class of deep-sea fish with nutritional value and health benefits. It tastes delicious and is deeply loved by consumers. [0003] At present, the most common nucleic acid detection technology related to animal product adulteration screening internationally and domestically is to use Sanger sequencing method, PCR (fluorescence quantitative PCR) method, combined with electrophoresis (or enzyme ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6869
CPCC12Q1/6888C12Q1/6869C12Q2600/158C12Q2600/178
Inventor 陈颖邢冉冉王楠张九凯于宁
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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