A kind of human sepsis pathogen detection kit and detection method

Abstract

The invention provides a detection kit for human sepsis pathogens and a corresponding detection method. The kit of the present invention includes detection of Acinetobacter baumannii, Klebsiella pneumoniae, Streptococcus pneumoniae, Bacteroides fragilis, Staphylococcus aureus, Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli bacteria, Enterococcus gallinarum, Enterobacter cloacae, Stenotrophomonas maltophilia, Coagulase-negative Staphylococcus, Candida albicans, Candida tropicalis, Candida glabrata, Cytomegalovirus, Human herpesvirus type IV , Human parvovirus B19, Candida parapsilosis, Streptococcus primers and fluorescent probes.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, and in particular, the invention relates to a detection kit for human sepsis pathogens and a corresponding detection method. Background technique [0002] Sepsis is a syndrome in which infection causes dysregulation of the host response and leads to life-threatening organ dysfunction. It is a clinical syndrome with a high mortality rate. Sepsis not only seriously threatens human health, but also brings a huge economic burden to health care. The mortality rate of sepsis in relevant studies in my country is higher than that in developed countries. A population-based epidemiological survey of sepsis found that 1,025,997 patients died of sepsis nationwide in 2015, accounting for 12.6% of the overall case fatality rate. Another single-center retrospective study of 759 patients with severe sepsis from 2006 to 2016 showed a fatality rate as high as 36.6%. [0003] In May 2018, Chi...

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Problems solved by technology

At the same time, the positive rate of blood culture will be affected by factors such as blood collection volume, time of blood collection, frequency of blood collection, etc., which will interfere with the positive rate of blood culture in sepsis

In addition, it takes a long time to diagnose sepsis by blood culture, usually 48 to 72 hours

(2) Blood routine: It is currently considered clinically that white blood cells are 9 / L or greater than >20×10 9 / L should be highly suspected of infection, however, studies have shown that 50% of neonatal sepsis children's white blood cells can be within the normal range, and abnormal white blood cell counts can also appear in other pathological conditions, so the white blood cell count is very important for sepsis infection Diagnosis lacks sensitivity and specificity

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