Application of butyric acid bacteria in the preparation of medicines for preventing and/or treating Kawasaki disease

A technology of butyric acid bacteria and Kawasaki disease, applied in the field of medicine, can solve the problems of butyric acid bacteria that have not yet been discovered, and achieve the effects of enhancing intestinal barrier function, reducing vascular inflammation, and reducing the level of inflammatory factors

Active Publication Date: 2022-07-19
浙江汉和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no information has been found about the application of butyric acid bacteria in the treatment of Kawasaki disease

Method used

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  • Application of butyric acid bacteria in the preparation of medicines for preventing and/or treating Kawasaki disease
  • Application of butyric acid bacteria in the preparation of medicines for preventing and/or treating Kawasaki disease
  • Application of butyric acid bacteria in the preparation of medicines for preventing and/or treating Kawasaki disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Preparation of Candida albicans Cell Wall Water Soluble (CAWS)

[0045] ①Strain: Candida albicans strain ATCC18804 (purchased from Ningbo Mingzhou Biotechnology Co., Ltd.).

[0046] ②Cultivation of Candida albicans: The strains were stored in glycerol at -80℃. Two days before the extraction step, a disposable inoculating loop was used to inoculate Sabouraud's medium (C-limiting medium, Qingdao Haibo), and cultured at 37°C to form a single colony for later use.

[0047] ③ Prepare 5×1L C-limiting medium, inoculate Candida albicans in C-limiting medium added with 200 μl biotin solution, and expand the bacteria by shaking at 27°C at 270 rpm for 2 days.

[0048] ④Add an equal volume of ethanol, and put it into the refrigerator at 4°C for alcohol precipitation overnight.

[0049] ⑤ 4 ℃, 9000rmp, 15min centrifugation, collect the precipitate, dissolve in 250ml purified water, and fully stir the precipitate in the rotor for 2h.

[0050] ⑥ Centrifuge the complex again, 4°C...

Embodiment 2

[0062] The therapeutic effect of butyric acid bacteria on Kawasaki disease mice was evaluated by detecting different indicators.

[0063] (1) By performing pathological sections and HE staining (hematoxylin-eosin staining, hematoxylin-eosin staining) on ​​the cardiac tissue of each group of mice mentioned in Example 1, the coronary artery of each group of mice was observed. , wherein, those skilled in the art can understand that the HE staining performed on the mouse heart tissue section above is a technique commonly used in the art, which is not more specifically limited in the embodiment of the present invention.

[0064] like figure 1 Shown are the results of HE staining of the coronary artery of the heart tissue of the mice in each group. The results show that the coronary vascular endothelium of the heart tissue of the mice in the PBS group has no swelling, continuous elastic fibers, and no infiltration of inflammatory cells. In the CAWS group, the coronary endothelial c...

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Abstract

The invention discloses the application of butyric acid bacteria in the preparation of medicines for preventing and / or treating Kawasaki disease, and belongs to the technical field of medicine. The present invention verifies through experiments that butyric acid bacteria improves intestinal flora, on the one hand, upregulates the expression levels of intestinal tight junction proteins Claudin-1, Jam-1, Occludin and ZO-1, enhances intestinal barrier function, reduces intestinal leakage, and reduces IL On the other hand, it increases the number of short-chain fatty acid-producing bacteria, increases the production of SCFAs, especially butyric acid, and up-regulates the level of monocarboxylic acid transporters, promotes the absorption of SCFAs, and improves the body’s anti-inflammatory properties. Inflammation ability to reduce vascular inflammation, play a role in the prevention and treatment of Kawasaki disease.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of a butyric acid bacillus in preparing a medicine for preventing and / or treating Kawasaki disease. Background technique [0002] Kawasaki disease (KD) is the most common acquired cardiovascular disease in children, with systemic small and medium vasculitis as the main pathological change. If not treated in time, Kawasaki disease can cause serious cardiovascular complications, such as coronary artery aneurysm, thrombosis, and even sudden death. Therefore, it is very necessary to provide a drug for the treatment of Kawasaki disease. [0003] Studies have shown that in the acute phase of KD, laboratory tests usually show a high inflammatory state of the blood, such as high white blood cell count, C-reactive protein, and erythrocyte sedimentation rate. The gut microbiota is significantly correlated with a variety of inflammatory diseases, and its metabolites short-chain fat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/742A61P9/00A61P29/00
CPCA61K35/742A61P29/00A61P9/00
Inventor 王方岩褚茂平钱范宇周锦慧章启豪赵检
Owner 浙江汉和生物科技有限公司
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