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Preparation method of chitosan immobilized microbial urease in-situ reinforced recycled aggregate

A recycled aggregate and in-situ strengthening technology, which is applied in the field of concrete, can solve the problems of weak bonding on the surface of recycled aggregate, affecting the performance of recycled aggregate, and uneven distribution of calcium carbonate, so as to improve mineralization efficiency, performance improvement, and water absorption. The effect of rate and crush index reduction

Active Publication Date: 2021-08-27
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new method uses tiny bacteria that can be activated into small pieces called shellfish cells or cellular fragments (chitons) on their own). These particles help break up old materials like concrete without consuming too much fuel for them to work properly. They also promote efficient formation of an organized matrix made from these components through chemical bondings between each other. Overall this process helps reduce excessive use of resources while improving the quality of demolished structures produced during remolded manufacturing processes.

Problems solved by technology

This patents discusses different ways that recycle materials (RCM) used during demolition projects or civil constructions works could contain residual dirt called clay sands, which causes issues when adding new RCM into concretes produced through conventional processes involving physical milling techniques alone. Existing solutions involve modifying existing building material surfaces with cementitious resin coatings, plaster reinforcement, alkali soluble salts, polysaccharides, polyvinyl alcohol crosslinkage agents, and various types of biological enzyme treatments. These modifications aimed to reduce these drawbacks while maintaining good workability and reducing negative effects associated with traditional remoldment procedures.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation:

[0023] 1. Preparation of recycled aggregate:

[0024] The waste concrete is preliminarily crushed with a press, and then crushed with a jaw crusher to screen out recycled aggregates with a particle size of 10-20mm.

[0025] 2. Preparation of chitosan solution:

[0026] It is prepared by dissolving acetic acid and chitosan powder in water, and the acetic acid and chitosan respectively account for 1 wt% of the chitosan solution in the formed chitosan solution.

[0027] 3. Preparation of microbial liquid:

[0028] Preparation of microbial culture medium: Weigh 20g of yeast extract, measure 950mL of deionized, mix well, adjust the pH value to 8 with 1mol / L sodium hydroxide, adjust the volume to 1L, prepare yeast extract medium, 121°C Sterilize for 15 minutes, cool to room temperature, then weigh 20 g of sterilized urea and add yeast extract medium to mix to obtain microbial culture medium.

[0029] The number of the sarcina pasteuriani strain is CGMCC1...

Embodiment 2

[0042] 1. Preparation:

[0043] 1. Preparation of recycled aggregate:

[0044] The waste concrete is preliminarily crushed with a press, and then crushed with a jaw crusher to screen out recycled aggregates with a particle size of 5-10mm.

[0045] 2. Preparation of chitosan solution:

[0046] It is prepared by dissolving acetic acid and chitosan powder in water, and the acetic acid and chitosan respectively account for 0.5wt% of the chitosan solution in the formed chitosan solution.

[0047] 3. Preparation of microbial liquid:

[0048] Preparation of microbial culture medium: Weigh 20g of yeast extract, measure 950mL of deionized, mix well, adjust the pH value to 8 with 1mol / L sodium hydroxide, adjust the volume to 1L, prepare yeast extract medium, 121°C Sterilize for 15 minutes, cool to room temperature, then weigh 20 g of sterilized urea and add yeast extract medium to mix to obtain microbial culture medium.

[0049] The number of the sarcina pasteuriani strain is CGMCC1...

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Abstract

The invention relates to a preparation method of a chitosan immobilized microbial urease in-situ reinforced recycled aggregate, which belongs to the technical field of concrete. The preparation method comprises the following steps: soaking the recycled aggregate in a chitosan solution formed by mixing chitosan powder, acetic acid and water and then drying, and obtaining the recycled aggregate with the surface covered with a chitosan film; inoculating sporosarcina pasteurii into a microorganism culture medium for culturing to obtain a microorganism bacterium solution; soaking the recycled aggregate of which the surface is coated with the chitosan film in a microbial liquid to obtain the recycled aggregate of which the surface adsorbs microbial urease; and soaking the recycled aggregate with the microbial urease adsorbed on the surface in a mixed solution which is composed of urea, calcium chloride and water and has a pH value of 9-10, and drying to obtain the reinforced recycled aggregate. The performance of the prepared chitosan immobilized microbial urease in-situ reinforced recycled aggregate is remarkably improved, and the water absorption rate and the crushing index are remarkably reduced compared with those before treatment.

Description

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Claims

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Application Information

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Owner YANGZHOU UNIV
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