Preparation method of chitosan immobilized microbial urease in-situ reinforced recycled aggregate
A recycled aggregate and in-situ strengthening technology, which is applied in the field of concrete, can solve the problems of weak bonding on the surface of recycled aggregate, affecting the performance of recycled aggregate, and uneven distribution of calcium carbonate, so as to improve mineralization efficiency, performance improvement, and water absorption. The effect of rate and crush index reduction
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Embodiment 1
[0022] 1. Preparation:
[0023] 1. Preparation of recycled aggregate:
[0024] The waste concrete is preliminarily crushed with a press, and then crushed with a jaw crusher to screen out recycled aggregates with a particle size of 10-20mm.
[0025] 2. Preparation of chitosan solution:
[0026] It is prepared by dissolving acetic acid and chitosan powder in water, and the acetic acid and chitosan respectively account for 1 wt% of the chitosan solution in the formed chitosan solution.
[0027] 3. Preparation of microbial liquid:
[0028] Preparation of microbial culture medium: Weigh 20g of yeast extract, measure 950mL of deionized, mix well, adjust the pH value to 8 with 1mol / L sodium hydroxide, adjust the volume to 1L, prepare yeast extract medium, 121°C Sterilize for 15 minutes, cool to room temperature, then weigh 20 g of sterilized urea and add yeast extract medium to mix to obtain microbial culture medium.
[0029] The number of the sarcina pasteuriani strain is CGMCC1...
Embodiment 2
[0042] 1. Preparation:
[0043] 1. Preparation of recycled aggregate:
[0044] The waste concrete is preliminarily crushed with a press, and then crushed with a jaw crusher to screen out recycled aggregates with a particle size of 5-10mm.
[0045] 2. Preparation of chitosan solution:
[0046] It is prepared by dissolving acetic acid and chitosan powder in water, and the acetic acid and chitosan respectively account for 0.5wt% of the chitosan solution in the formed chitosan solution.
[0047] 3. Preparation of microbial liquid:
[0048] Preparation of microbial culture medium: Weigh 20g of yeast extract, measure 950mL of deionized, mix well, adjust the pH value to 8 with 1mol / L sodium hydroxide, adjust the volume to 1L, prepare yeast extract medium, 121°C Sterilize for 15 minutes, cool to room temperature, then weigh 20 g of sterilized urea and add yeast extract medium to mix to obtain microbial culture medium.
[0049] The number of the sarcina pasteuriani strain is CGMCC1...
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