Preparation method of chitosan immobilized microbial urease in-situ reinforced recycled aggregate

Abstract

The invention relates to a preparation method of a chitosan immobilized microbial urease in-situ reinforced recycled aggregate, which belongs to the technical field of concrete. The preparation method comprises the following steps: soaking the recycled aggregate in a chitosan solution formed by mixing chitosan powder, acetic acid and water and then drying, and obtaining the recycled aggregate with the surface covered with a chitosan film; inoculating sporosarcina pasteurii into a microorganism culture medium for culturing to obtain a microorganism bacterium solution; soaking the recycled aggregate of which the surface is coated with the chitosan film in a microbial liquid to obtain the recycled aggregate of which the surface adsorbs microbial urease; and soaking the recycled aggregate with the microbial urease adsorbed on the surface in a mixed solution which is composed of urea, calcium chloride and water and has a pH value of 9-10, and drying to obtain the reinforced recycled aggregate. The performance of the prepared chitosan immobilized microbial urease in-situ reinforced recycled aggregate is remarkably improved, and the water absorption rate and the crushing index are remarkably reduced compared with those before treatment.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

AI Technical Summary

Benefits of technology

This new method uses tiny bacteria that can be activated into small pieces called shellfish cells or cellular fragments (chitons) on their own). These particles help break up old materials like concrete without consuming too much fuel for them to work properly. They also promote efficient formation of an organized matrix made from these components through chemical bondings between each other. Overall this process helps reduce excessive use of resources while improving the quality of demolished structures produced during remolded manufacturing processes.

Problems solved by technology

This patents discusses different ways that recycle materials (RCM) used during demolition projects or civil constructions works could contain residual dirt called clay sands, which causes issues when adding new RCM into concretes produced through conventional processes involving physical milling techniques alone. Existing solutions involve modifying existing building material surfaces with cementitious resin coatings, plaster reinforcement, alkali soluble salts, polysaccharides, polyvinyl alcohol crosslinkage agents, and various types of biological enzyme treatments. These modifications aimed to reduce these drawbacks while maintaining good workability and reducing negative effects associated with traditional remoldment procedures.