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Measuring method of plant real-time water potential

A measuring method and technology of water potential, applied in the fields of agricultural engineering and crop information detection, can solve the problems of long measuring time, cumbersome and complicated operation steps, high measuring technical requirements, etc., and achieve the effect of convenient control and simple operation.

Active Publication Date: 2022-06-24
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these techniques can measure the water potential of plants relatively accurately, most of the methods in the test methods have cumbersome and complicated operation steps, high requirements for measurement technology, and relatively long measurement time, and need to rely on expensive experimental instruments to complete the test. Suitable for water potential measurement of large-scale agricultural and forestry plants in the field

Method used

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  • Measuring method of plant real-time water potential
  • Measuring method of plant real-time water potential
  • Measuring method of plant real-time water potential

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The method for measuring real-time water potential of herbaceous plant wheat includes the following steps:

[0067] (a) Water-saturated treatment of herbaceous plant wheat: After fully watering the wheat in the early morning, and using wet tissue paper to completely wet the inner wall of the black plastic bag, wrap all the wheat leaves for 60 minutes, so that the wheat is fully hydrated After the leaf water reaches saturation, and the water potential value of the plant is measured by the pressure chamber method to ensure that the water potential value Ψmax=0; 5 pieces of healthy growing wheat leaves are randomly selected, and the leaves are tiled on the cardboard. Dmax);

[0068] (b) natural shade-drying treatment of leaves: the leaves are quickly placed in the indoor natural shade-drying treatment, according to the water potential Ψtlp of the turgor loss point of the species, the water potential of the plant leaves (Ψx) and the real-time widest width of the leaves (Dx)...

Embodiment 2

[0073] The method for measuring the real-time water potential of the woody woody oak, including the following steps:

[0074] (a) Water absorption and saturation treatment of Quercus nigra: After fully watering Quercus nigra in the early morning, and using wet tissue paper to completely wet the inner wall of the black plastic bag, wrap all the leaves of Quercus nirvana for 60 minutes, so that Quercus nigra is fully hydrated. After the synergy, the leaf water reached saturation, and the water potential value of the plant was measured by the pressure chamber method to ensure that the water potential value Ψmax = 0; 5 healthy green oak leaves were randomly selected, and the leaves were laid flat on the cardboard, and the vernier calipers were used to measure the width of the water-saturated leaves. width (Dmax);

[0075] (b) natural shade-drying treatment of leaves: the leaves are quickly placed in the indoor natural shade-drying treatment, according to the water potential Ψtlp o...

experiment example

[0080] The results of the accuracy and applicability of the relationship equation between relative leaf shrinkage and leaf water potential are shown in Tables 1-2. Figures 1 to 2 shown. Among them, Table 1 shows the water potential and the corresponding shrinkage value during the natural drying process of wheat, and Table 2 shows the water potential and the corresponding shrinkage value during the natural drying process of Quercus chinensis.

[0081] The water potential and relative shrinkage of the herbaceous plant wheat, and the water potential and relative shrinkage of the woody plant Quercus japonica were measured, and nonlinear fitting was performed respectively. Among them, the sigmoidal equation fitting of the relationship between wheat leaf shrinkage and water potential: Ds=36.6774 / (1+e (Ψx+2.3579) / 0.4671 ), R 2 =0.9526, Pfigure 1 ; sigmoidal equation fitting of the relationship between leaf shrinkage and water potential of Quercus japonica: Ds=35.7492 / (1+e (Ψx+3.6...

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Abstract

The invention relates to the technical field of agricultural engineering and crop information detection, in particular to a method for measuring real-time water potential of plants. The method for measuring the real-time water potential of plants of the present invention comprises the following steps: (a) after the plants are saturated with water absorption, measure the width Dmax of the widest part of the leaves; (b) carry out the natural shade-drying treatment of the leaves, Before the turgor pressure loss point, measure the plant leaf water potential Ψx and the real-time widest width Dx of the leaves every 4 to 6 minutes; after the leaves reach the turgor pressure loss point, measure the plant leaf water potential Ψx and the real-time maximum width of the leaves every 50 to 70 minutes. Width Dx at the widest point; (c) measure the width Dmin at the widest point of the blade after the blade tested in step (b) is completely dehydrated; construct the relationship equation between the relative shrinkage rate Ds of the blade and the water potential Ψx of the blade. The method of the invention does not cause damage to the test plants, and the operation is simple and fast.

Description

technical field [0001] The invention relates to the technical fields of agricultural engineering and crop information detection, in particular to a method for measuring the real-time water potential of plants. Background technique [0002] Global climate change leads to increasing intensity and frequency of drought events, which will have a significant impact on the growth and yield of agricultural and forestry plants. Plant water potential is an important physiological indicator to characterize plant water deficit. By understanding the real-time water potential energy of plants, it provides important scientific guidance for the implementation of scientific irrigation, promoting the growth of agricultural and forestry plants and stabilizing yield and increasing income. [0003] At present, the techniques for measuring plant water potential mainly include small liquid flow method, pressure chamber method, thermocouple method and xylem pressure probe method. Although these te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 薛亮李生王佳
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY