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Immunosuppressive myocarditis mouse model and construction method and application thereof

A mouse model, immunosuppressive technology, applied in the field of applied basic medical research, can solve the problems of dilated cardiomyopathy, heart failure, non-specific clinical manifestations, non-specific treatment methods, etc.

Active Publication Date: 2021-09-14
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Myocarditis (Myocarditis) is a common inflammatory disease of the myocardium. The incidence has been on the rise in recent years, and the clinical manifestations are non-specific. It is easy to be ignored and lead to serious consequences such as dilated cardiomyopathy and heart failure. According to statistics, about 21% of myocarditis Patients can develop dilated cardiomyopathy, in which in up to 12% of heart disease cases, myocarditis is thought to be the cause of sudden cardiac death in young people, but there is currently no specific clinical treatment
[0003] At present, animal models of myocarditis at home and abroad mainly include cytomegalovirus-induced and Coxsackie virus-induced mouse models. Domestic myocarditis models mostly use 6-8-week-old male BALB / c mice that are relatively susceptible to Coxsackie virus. Constructing models through viruses has biological safety hazards and high mortality

Method used

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  • Immunosuppressive myocarditis mouse model and construction method and application thereof
  • Immunosuppressive myocarditis mouse model and construction method and application thereof
  • Immunosuppressive myocarditis mouse model and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of normal control group, positive control group and model group

[0043] 1) Experimental animals

[0044] 25 SPF-grade BALB / c mice, male, 4-6 weeks old, weighing 16-20 g, were purchased from the Experimental Animal Center of Hunan University of Traditional Chinese Medicine and bred in the Experimental Animal Center of Hunan University of Traditional Chinese Medicine [SCXK (Xiang) 2019-0009 】. During the feeding period, the rats in each group drank water freely and were fed with common maintenance feed, which was provided by the Experimental Animal Center of Hunan University of Traditional Chinese Medicine. Breeding environment: Day and night half-cycle lighting, humidity 40-60%, temperature control at 20-25°C, light / dark cycle of 12 hours. All operations complied with the experimental ethics requirements of Hunan University of Traditional Chinese Medicine [LL2020120202].

[0045] 2) Experimental drugs and reagents

[0046] Cyclosporin A (Cyclos-porin A, ...

Embodiment 2

[0059] Example 2 HE staining observation of pathological changes in mouse myocardium and detection of various indicators

[0060] 1) Animal handling

[0061] One day after the last injection, all surviving mice were sacrificed by cervical dislocation for specimen collection. Routinely fix, disinfect, and open the chest of the mice, separate the thymus, spleen, lung, liver, kidney and heart, take out the heart and put it in 4°C pre-cooled normal saline immediately, rinse the residual blood, and then dry it with filter paper , the apical tissue was fixed in conventional 4% paraformaldehyde, and the rest of the tissue was stored at -80° for long-term preservation.

[0062] 2) Thymus index calculation

[0063] The electronic balance weighs the heart, thymus, spleen, and lungs, and then calculates the organ mass index, where organ mass index=organ mass / body mass.

[0064] For the thymus index of the blank control group, model group, and positive control group, see figure 2 . ...

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Abstract

The invention provides an immunosuppressive myocarditis mouse model and a construction method and application thereof. The method comprises the following steps that a male BALB / c mouse of 4-6 weeks old is selected as an experimental mouse for constructing the myocarditis mouse model, and observation and feeding are conducted for three days; after feeding is conducted for three days, a cyclosporine A solution with the preset concentration is injected in an intraperitoneal injection mode, the injection amount each time is 40-50mg / kg of the mouse weight, injection is conducted once every other day, and injection is conducted three times in total; and after the last injection is conducted for one day, the myocarditis mouse model is obtained. According to the immunosuppressive myocarditis mouse model and the construction method and application thereof, cyclosporine A serves as an immunosuppressor, myocarditis is successfully induced according to a specific administration method, myocardial damage is caused, the myocarditis mouse model is constructed, and death does not exist; compared with a mouse model constructed through virus infection and LPS induced inflammation in the prior art, the immunosuppressive myocarditis mouse model has the advantages of being safe and effective, and the tolerance of the experimental mouse is better.

Description

technical field [0001] The invention belongs to the field of applied basic medical research, in particular to an immunosuppressive myocarditis mouse model and its construction method and application. Background technique [0002] Myocarditis (Myocarditis) is a common inflammatory disease of the myocardium. The incidence rate has been on the rise in recent years, and the clinical manifestations are non-specific. It is easy to be ignored and lead to serious consequences such as dilated cardiomyopathy and heart failure. According to statistics, about 21% of myocarditis Patients can develop dilated cardiomyopathy, in which in up to 12% of heart disease cases, myocarditis is considered to be the cause of sudden cardiac death in young people, but there is currently no specific clinical treatment. [0003] At present, animal models of myocarditis at home and abroad mainly include cytomegalovirus-induced and Coxsackie virus-induced mouse models. Domestic myocarditis models mostly us...

Claims

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Application Information

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IPC IPC(8): A61K38/13A01K67/027
CPCA61K38/13A01K67/027A01K2267/0368A01K2267/0375Y02A50/30
Inventor 魏科赵天浩陈凯琴卢芳国蒋艺轩徐琰喆邱丹
Owner HUNAN UNIV OF CHINESE MEDICINE
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