Movable miRNAs and identification methods for promoting root development and drought resistance of rootstocks
A rootstock and root system technology, applied in the fields of biotechnology and genetic improvement, can solve the problems of inability to connect miRNAs, low grafting success rate of heterologous species, and inconvenient verification of miRNA movement experiments
Active Publication Date: 2022-07-29
NORTHWEST A & F UNIV
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Problems solved by technology
[0006] Through the above analysis, the existing problems and defects of the prior art are as follows: 1. Most of the prior art is researched by sequencing analysis of phloem sap after grafting, in situ hybridization, stem-loop RT-qPCR, mutant screening and Northern blot. The movement of miRNAs, the above experiments can discover new mobile miRNAs, but there are few reports on the biological functions of miRNAs after movement
Second, miRNAs will be sheared twice during processing and generation, so fluorescent tags (such as GFP) cannot be attached to accurately visualize the movement of miRNAs
Third, the success rate of heterologous species grafting is low, and the miRNA family is very conserved among different species, which brings inconvenience to the experimental verification of miRNA movement
The signal transduction mechanism and regulation mechanism between cells and organs mediated by miRNA are less studied
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[0076] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.
[0077] In view of the problems existing in the prior art, the present invention provides a mobile miRNA and an identification method for promoting rootstock root development and drought resistance. The present invention is described in detail below with reference to the accompanying drawings.
[0078] The mobile miRNA that promotes rootstock root development and drought resistance provided in the embodiment of the present invention is Mdm-miR160 in apple.
[0079] like figure 1 As shown, the identification method of the mobile miRNA that promotes rootstock root development and drought resistance provided by the em...
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The invention belongs to the technical field of biotechnology and genetic improvement, and discloses a mobile miRNA for promoting root development and drought resistance of rootstocks and an identification method, wherein the mobile miRNA for promoting root development and drought resistance of rootstocks is Mdm-miR160 in apples; The identification methods of mobile miRNAs that promote root development and drought resistance of rootstocks include: RNA extraction and reverse transcription; gene cloning; apple genetic transformation; drought stress treatment; in situ hybridization; micrografting; tobacco grafted tomato; data analysis. The mobile miRNA that promotes root development and drought resistance of rootstocks provided by the present invention is Mdm-miR160 in apples, which can move from scions to rootstocks to promote rootstock development, thereby improving the drought resistance of apples. The present invention obtains overexpressed transgenic lines through apple genetic transformation, and phenotype analysis finds that Mdm-miR160e OE exhibits dwarf symptoms.
Description
technical field [0001] The invention belongs to the technical field of biotechnology and genetic improvement, and in particular relates to a mobile miRNA and an identification method for promoting rootstock root development and drought resistance. Background technique [0002] Currently, plants have evolved dynamic and complex intercellular communication networks that coordinate and adapt their growth and development to various environmental changes. In addition to macromolecules such as proteins and RNAs, small molecules such as metabolites and plant hormones also play a signaling role in plants. As a signal molecule, RNA can move locally between cells through plasmodesmata, or it can play a biological function through long-distance movement of phloem. Non-cell autonomous RNAs can act as mobile signals for various physiological processes such as plant growth and development, nutrient allocation, gene silencing, antiviral defense, and stress response, and have important bio...
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IPC IPC(8): C12N15/113C12N15/84A01H5/06A01H5/00A01H6/74C12Q1/6895C12Q1/6841A01G2/30
CPCC12N15/113C12N15/8205C12N15/8218C12N15/8273C12N15/8261C12Q1/6895C12Q1/6841A01G2/30C12N2310/141C12Q2600/158C12Q2600/178C12Q2600/13C12Q2525/207
Inventor 管清美申小霞毛秀山平怡坤李雪薇樊天乐张子桐
Owner NORTHWEST A & F UNIV