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Method for microbial inoculum and wheat breeding

A technology of microorganisms and inoculum, applied in the fields of botanical equipment and methods, chemicals for biological control, horticultural methods, etc., can solve problems such as doubts about the colonization effect, and achieve the improvement of microbial polymorphism and high colonization ability. Effect

Active Publication Date: 2022-06-07
GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose of these operations is to hope that the exogenous bacteria can survive and colonize the root system, interior and surface of the plant, but due to the soil environment, climatic factors and local bacteria, the colonization effect is questionable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, a kind of microbial inoculum

[0031] A microbial inoculum, comprising the following components by weight:

[0032] Boric acid 0.005%; calcium nitrate 0.005%; sodium carboxymethyl cellulose 0.01% and arabinose 0.005%, the microorganism inoculum also includes a concentration of 10 5 cfu / ml microorganism, the microorganism is Burkholderia, and the balance is water.

[0033] The preparation method of above-mentioned microbial inoculum, comprises the following steps:

[0034] Each raw material is taken according to weight percentage, sodium carboxymethyl cellulose is dissolved in water, boric acid and calcium nitrate are added to dissolve, then arabinose is added, and finally microorganisms are added and mixed to obtain a microorganism inoculum.

Embodiment 2

[0035] Embodiment 2, a kind of microbial inoculum

[0036] A microbial inoculum, comprising the following components by weight:

[0037] Boric acid 0.02%; calcium nitrate 0.01%; sodium carboxymethyl cellulose 0.02% and arabinose 0.2%, the microorganism inoculum also includes a concentration of 10 10 cfu / ml microorganisms, the microorganisms are Acetobacter nitrogen-fixing, and the balance is water.

[0038] The preparation method of above-mentioned microbial inoculum, comprises the following steps:

[0039]Each raw material is taken according to weight percentage, sodium carboxymethyl cellulose is dissolved in water, boric acid and calcium nitrate are added to dissolve, then arabinose is added, and finally microorganisms are added and mixed to obtain a microorganism inoculum.

Embodiment 3

[0040] Embodiment 3, a kind of microbial inoculum

[0041] A microbial inoculum, comprising the following components by weight:

[0042] Boric acid 0.01%; calcium nitrate 0.01%; sodium carboxymethyl cellulose 0.02% and arabinose 0.01%, the microorganism inoculum also includes a concentration of 10 8 cfu / ml microorganism, the microorganism is Burkholderia, and the balance is water.

[0043] The preparation method of above-mentioned microbial inoculum, comprises the following steps:

[0044] Each raw material is taken according to weight percentage, sodium carboxymethyl cellulose is dissolved in water, boric acid and calcium nitrate are added to dissolve, then arabinose is added, and finally microorganisms are added and mixed to obtain a microorganism inoculum.

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Abstract

The invention relates to the technical field of plant cultivation, and specifically discloses a microbial inoculum solution and a wheat breeding method. The microbial inoculum of the present invention comprises the following components in weight percent: 0.005% to 0.02% of boric acid; 0.005% to 0.01% of calcium nitrate; 0.01% to 0.02% of sodium carboxymethylcellulose and 0.005% to 0.2% of arabinose, The microbial inoculum also includes a concentration of 10 5 ~10 10 Cfu / ml of microorganisms, microorganisms include at least one of Burkholderia and nitrogen-fixing acetic acid bacteria, and the balance is water. The invention can add microorganisms to the seed production process, so that the microorganisms are introduced into the seeds, increase the microorganisms in the wheat seeds, and improve the microbial polymorphism of the seeds. The wheat breeding method of the invention has high colonization ability of the microorganisms, the microorganisms are easy to survive, can spread to the whole body of the wheat, and are beneficial to the growth of the wheat.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a microbial inoculum and a method for wheat breeding. Background technique [0002] Wheat is divided into winter wheat and spring wheat. Taking winter wheat as an example, soil preparation and fertilization begin in September and October, and sowing is carried out when the local moisture content is suitable. It is necessary to prevent and control underground pests and seed-borne diseases in time. At the beginning of November, the wheat enters the tillering stage, at this time, it is necessary to promote root growth and cultivate strong seedlings. After the wintering period of wheat is over, about late February of the following year, the wheat will turn green and jointed, and at this time, a strong stem should be prevented from falling. When wheat enters the stage of booting, heading, and flowering, it is generally necessary to strengthen the management of fertilizer an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01H1/00A01G7/06A01N63/20A01N59/14A01N43/16A01N43/08A01P21/00
CPCA01H1/00A01G7/06A01N63/20A01N59/14A01N43/16A01N43/08
Inventor 李华一杨效帆罗钰彬
Owner GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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