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Liquid-solid two-phase fermentation method for saccharomyces cerevisiae used for feed

A technology of Saccharomyces cerevisiae and fermentation method, which is applied in the field of fermentation and achieves the effects of low production cost, strong tolerance and solving high energy consumption

Inactive Publication Date: 2016-03-23
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the shortage of liquid fermentation and solid fermentation process of Saccharomyces cerevisiae for feeding, optimizes the fermentation process, and provides a liquid-solid two-phase fermentation method for Saccharomyces cerevisiae for feeding

Method used

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Experimental program
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Effect test

Embodiment 1

[0051] 1. Fermentation test

[0052] The feed additive is prepared by fermenting Saccharomyces cerevisiae by a liquid-solid two-phase process, and the specific steps are as follows:

[0053] (1) Activation and rejuvenation of bacteria

[0054] The preserved Saccharomyces cerevisiae (strain preservation number ACCC20065) freeze-dried tube bacterial strain was made into a bacterial suspension with sterile water, and the bacterial liquid was diluted to 10 -8 24 hours later, pick a single colony and carry out streak isolation and culture again to obtain a single colony.

[0055] (2) preparation of slant bacteria

[0056] The formula of the slant medium is as follows: wort juice 12Bx, agar 2%, constant volume to 1L, natural pH;

[0057] Heat the slant medium to dissolve, then divide and seal the test tubes; sterilize the test tubes at 121°C and 0.15 MPa for 30 minutes, take them out and cool them for later use;

[0058] The above slant culture medium was inserted into the strai...

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Abstract

The invention relates to the field of fermentation, and concretely relates to a liquid-solid two-phase fermentation method for saccharomyces cerevisiae used for a feed. The method comprises the following steps: activating a strain and rejuvenating the strain; preparing slant strains; preparing a liquid medium in a 500 mL Erlenmeyer flask; enlarging the preparation of the strain in a 1000 mL Erlenmeyer flask; preparing the strain in a seed tank, and performing solid fermentation. The fermentation method can effectively reduce contamination risk of liquid fermentation during an inoculation process, the percentage of contaminating microorganism is 0.5%, seed preparation time can be shortened, solid advantage can be exploited, problems of high energy consumption and difficult post-treatment can be solved, the fermentation medium is optimized, and effective active bacteria number of the fermentation finished product can be increased to 10 billion / gram.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a liquid-solid two-phase fermentation method of Saccharomyces cerevisiae for feeding. Background technique [0002] With the rapid development of my country's animal husbandry, Saccharomyces cerevisiae, as a probiotic, is more and more widely used in the animal husbandry industry. However, the current production of Saccharomyces cerevisiae mainly uses the liquid fermentation process. Although the fermentation process is relatively mature, it also has problems such as high energy consumption and easy pollution. The production of Saccharomyces cerevisiae using the traditional solid fermentation process has the problem of low effective number of viable bacteria. [0003] The liquid-solid two-phase fermentation method is mainly a process in which a large amount of seed liquid is obtained through liquid fermentation, and then inoculated into a solid medium for fermentation. This process c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/865
CPCC12N1/16
Inventor 邱权詹志春
Owner 湖北华扬科技发展有限公司
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