Porous scaffold as well as preparation method and application thereof
A technology of porous scaffolds and porous materials, used in prosthesis, tissue regeneration, medical science and other directions, can solve the problems of short life, easy inactivation, ectopic osteogenesis, etc., achieve good mineralization, good mechanical properties, promote bone repaired effect
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[0048] In this embodiment, a porous scaffold is prepared, and the specific process is as follows:
[0049] Calcium magnesium silicate (CMS) and graphene oxide (GO) were ultrasonicated in deionized water for 30 minutes in a mass ratio of 1:1 to obtain a CMS / GO suspension; then adding a silk fibroin solution with a mass concentration of 6%, After rapid stirring for 10 min, the prepared suspension was stored at -20°C for 12 h, and the scaffold was lyophilized under vacuum freezing. After the scaffold was immersed in ethanol for 4 h, the MES buffer containing 25 mM EDC and 50 mM NHS ( The molar concentration is 50nM; the solvent is ethanol with a volume fraction of 70%) soaking for 12h, and the mass / volume ratio of the soaking material is 0.1g / 10mL. After washing with deionized water for three times, the porous scaffold (CMS / GO / SF) was obtained by freeze-drying again.
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