Fibronectin-coated tannic acid/iron complex drug-loaded nano material and preparation and application thereof

A technology of drug-loading nanometer and fibronectin, which can be applied to preparations for in vivo experiments, medical preparations containing active ingredients, nanotechnology, etc. pH responsive performance, potential clinical application value, well-distributed effect

Active Publication Date: 2021-10-26
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Searching domestic and foreign literature and patents has not found anything about the preparation of drug-loaded nanomaterials of tannic acid / iron complexes coated with fibronectin and having MR imaging properties, and the anticancer drug DOX combined with ferroptosis loaded in its cavity for tumor T 1 Reports on MR Imaging and Combination Chemotherapy / Immune Therapy

Method used

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  • Fibronectin-coated tannic acid/iron complex drug-loaded nano material and preparation and application thereof
  • Fibronectin-coated tannic acid/iron complex drug-loaded nano material and preparation and application thereof
  • Fibronectin-coated tannic acid/iron complex drug-loaded nano material and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] (1) Weigh 3.48mg of doxorubicin hydrochloride and dissolve it in methanol (1mL), add triethylamine to deprotonate, wherein the molar ratio of doxorubicin hydrochloride to triethylamine is 1:1, after fully reacting for 4h Afterwards, hydrophobic doxorubicin (DOX) was obtained; the methanol solution of DOX was mixed with tannic acid (TA) (0.2mg / mL) dissolved in absolute ethanol and stirred, and ferric chloride (FeCl 3 ) aqueous solution (0.5mg / mL), the above mixed solution was stirred for 12h overnight, the methanol and ethanol were volatilized and removed, and centrifuged at 13000rpm at 4°C for 30min to obtain the drug-loaded nanomaterial of the tannic acid / iron complex loaded with doxorubicin (DOX-TAF), where TA / FeCl 3 The molar ratio of / DOX is 1:1:2.

[0079] (2) The drug-loaded nanomaterial (DOX-TAF) (1 mg) of doxorubicin-loaded tannic acid / iron complex was dissolved in ultrapure water (1.5 mL), and fibronectin solution (0.1 mg / mL, 5mL ultrapure water), mixed with ...

Embodiment 2

[0081] Take 1 mg of TAF synthesized in Comparative Example 1, DOX-TAF and DOX-TAF@FN synthesized in Example 1, and dilute each group of materials to 50 μg / mL with ultrapure water for the determination of surface potential and hydrodynamics diameter. As shown in Table 1, the potential of TAF was −24.5 mV and that of DOX-TAF was −18.2 mV, and the potential increased, and the particle size increased to 190.1 nm, demonstrating the successful loading of DOX. After bovine serum albumin is coated on the surface of DOX-TAF by physical extrusion, the potential drops to -29.2mV, and the hydrated particle size becomes 232.2nm; and after fibronectin is coated with hydrogen bonds, the potential becomes - 25.5mV, the hydrated particle size changed to 221.3nm, which proved the successful coating of fibronectin on the DOX-TAF surface. The hydrodynamic diameter of DOX-TAF@FN in water, PBS solution and RPMI 1640 medium can remain basically unchanged for a long time ( figure 2 ), proving that ...

Embodiment 3

[0085] Take TAF, DOX-TAF, DOX-TAF@FN prepared in Example 1 for ultraviolet characterization, as image 3 As shown, in the curves b and c, the absorption peak at 480nm is the characteristic peak of DOX, which proves the successful loading of DOX.

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Abstract

The invention relates to a fibronectin-coated tannic acid / iron complex drug-loaded nano material and a preparation method and application thereof. The method comprises the following steps: adding doxorubicin hydrochloride into triethylamine for deprotonation, mixing an obtained hydrophobic doxorubicin solution and a tannic acid solution, adding iron salt for reaction, and mixing, stirring and extruding an obtained drug-loaded nano material of the tannic acid / iron complex loaded with doxorubicin and a fibronectin solution. The drug-loaded nano material prepared by the method not only can realize T1MR imaging, but also can play an anti-tumor immune effect through immunogenic death caused by chemotherapy and ferroptosis, and has potential clinical application value.

Description

technical field [0001] The invention belongs to the field of nano-medicine diagnosis and treatment materials and their preparation and application, in particular to a drug-loaded nano-material of tannic acid / iron complex coated with fibronectin and its preparation method and application. Background technique [0002] Traditional tumor treatment has disadvantages such as high toxicity and side effects, poor selectivity, and poor curative effect. Accurate diagnosis and efficient treatment are crucial to improving the survival rate of cancer patients. Targeted delivery of traditional chemotherapy drugs through nanomaterials and combined treatment with multiple treatment modalities are effective ways to enhance the therapeutic effect. It is still a huge challenge to develop new and easy-to-prepare multifunctional nanocarrier materials to simultaneously load one or more contrast agents or therapeutic agents for efficient combined therapy and integration of diagnosis and treatment...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61K47/42A61P35/00A61K49/08A61K49/10B82Y5/00B82Y40/00
CPCA61K31/704A61K47/42A61P35/00A61K49/085A61K49/106B82Y5/00B82Y40/00
Inventor 史向阳徐瑶张昌昌
Owner DONGHUA UNIV
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