Application of kadsura coccinea extract in preparation of skin whitening product
A technology of leaf extract and tiger, which is applied in the application field of black tiger extract and Boluohui extract in the preparation of whitening products, to achieve good hypoglycemic activity and significant inhibitory effect
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Embodiment 1
[0045] 1. α-glucosidase activity inhibition experiment
[0046] 1.1. Enzyme inhibition kinetics experiment
[0047] Preparation of PBS buffer solution: Liquid A (0.1mol / L potassium dihydrogen phosphate): potassium dihydrogen phosphate 1.361g. Add double distilled water to 100ml. Solution B (0.1mo / L disodium hydrogen phosphate): 1.78g disodium hydrogen phosphate, add double distilled water to 100ml. Mix A liquid and B liquid 1:1.
[0048] Preparation of α-glucosidase mother solution: Dissolve 1.0 mg of α-glucosidase powder with an activity unit of 100 U / mg in 1.0 mL of PBS buffer to obtain 100 U / mL α-glucosidase mother solution. Take 0.10mL of the mother solution and dissolve it in 10mL of PBS buffer to make a 2U / mL α-glucosidase solution.
[0049] Preparation of positive control drug acarbose standard solution: take 0.3398g of acarbose in a 10ml volumetric flask, dissolve it in PBS and make it into a 50.0mmol / L mother solution, and dilute it gradually to 5.0mmol / L and 12.5m...
Embodiment 2
[0091] 1. Tyrosinase activity inhibition experiment
[0092] 1.1. Establishment of the experimental method for tyrosinase inhibition
[0093] 1.1.1. Preparation of experimental reagents
[0094] Preparation of PBS buffer solution: Liquid A (0.1mol / L potassium dihydrogen phosphate): potassium dihydrogen phosphate 1.361g. Add double distilled water to 100ml. Solution B (0.1mo / L disodium hydrogen phosphate): 1.78g disodium hydrogen phosphate, add double distilled water to 100ml. Mix A liquid and B liquid 1:1.
[0095] Preparation of L-tyrosine solution: Take 0.04g of tyrosine and add 100ml of PBS buffer to dissolve it.
[0096] Tyrosinase solution preparation: take 0.004g tyrosinase, add PBS buffer 100ml to dissolve it.
[0097] Vitamin C solution preparation: take 0.08g of vitamin C, add 100ml of deionized water to dissolve it.
[0098] Preparation of vitamin C with different concentration gradients: take the prepared 0.8mg / ml vitamin C solution and add deionized water, and...
Embodiment 3
[0142]In order to determine the chemical components in the black tiger extract that have hypoglycemic and whitening functions, we further analyzed and identified them.
[0143] 1. Analysis of chemical components of black tiger leaves
[0144] According to the fingerprints of various parts of the black tiger, it can be seen that the detection peaks of black tiger leaves are the most under liquid phase conditions, and the chemical components may be the most complex. )analyze.
[0145] 1.1. Experimental method
[0146] The chemical constituents of black tiger leaves were analyzed by liquid chromatography-mass spectrometry. Pass the alcohol extract of black tiger leaves through a 0.22 μm microporous membrane, and load the sample for LC-MS analysis.
[0147] Chromatographic conditions: using Eclipse Plus C18 chromatographic column (250mm×4.6mm, 5μm), mobile phase is acetonitrile (A)-0.1% formic acid water (B), gradient elution 0~5min, 12%A; 5~10min, 12 %A→20%A; 10~20min, 20%A; ...
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