Application of kadsura coccinea extract in preparation of skin whitening product

A technology of leaf extract and tiger, which is applied in the application field of black tiger extract and Boluohui extract in the preparation of whitening products, to achieve good hypoglycemic activity and significant inhibitory effect

Active Publication Date: 2021-10-29
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few literature reports on natural α-glucosidase activity inhibitors and tyrosinase activity inhibitors, and a variety of Chinese medicinal materials with hypoglycemic and whitening functions need to be developed urgently

Method used

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  • Application of kadsura coccinea extract in preparation of skin whitening product
  • Application of kadsura coccinea extract in preparation of skin whitening product
  • Application of kadsura coccinea extract in preparation of skin whitening product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. α-glucosidase activity inhibition experiment

[0046] 1.1. Enzyme inhibition kinetics experiment

[0047] Preparation of PBS buffer solution: Liquid A (0.1mol / L potassium dihydrogen phosphate): potassium dihydrogen phosphate 1.361g. Add double distilled water to 100ml. Solution B (0.1mo / L disodium hydrogen phosphate): 1.78g disodium hydrogen phosphate, add double distilled water to 100ml. Mix A liquid and B liquid 1:1.

[0048] Preparation of α-glucosidase mother solution: Dissolve 1.0 mg of α-glucosidase powder with an activity unit of 100 U / mg in 1.0 mL of PBS buffer to obtain 100 U / mL α-glucosidase mother solution. Take 0.10mL of the mother solution and dissolve it in 10mL of PBS buffer to make a 2U / mL α-glucosidase solution.

[0049] Preparation of positive control drug acarbose standard solution: take 0.3398g of acarbose in a 10ml volumetric flask, dissolve it in PBS and make it into a 50.0mmol / L mother solution, and dilute it gradually to 5.0mmol / L and 12.5m...

Embodiment 2

[0091] 1. Tyrosinase activity inhibition experiment

[0092] 1.1. Establishment of the experimental method for tyrosinase inhibition

[0093] 1.1.1. Preparation of experimental reagents

[0094] Preparation of PBS buffer solution: Liquid A (0.1mol / L potassium dihydrogen phosphate): potassium dihydrogen phosphate 1.361g. Add double distilled water to 100ml. Solution B (0.1mo / L disodium hydrogen phosphate): 1.78g disodium hydrogen phosphate, add double distilled water to 100ml. Mix A liquid and B liquid 1:1.

[0095] Preparation of L-tyrosine solution: Take 0.04g of tyrosine and add 100ml of PBS buffer to dissolve it.

[0096] Tyrosinase solution preparation: take 0.004g tyrosinase, add PBS buffer 100ml to dissolve it.

[0097] Vitamin C solution preparation: take 0.08g of vitamin C, add 100ml of deionized water to dissolve it.

[0098] Preparation of vitamin C with different concentration gradients: take the prepared 0.8mg / ml vitamin C solution and add deionized water, and...

Embodiment 3

[0142]In order to determine the chemical components in the black tiger extract that have hypoglycemic and whitening functions, we further analyzed and identified them.

[0143] 1. Analysis of chemical components of black tiger leaves

[0144] According to the fingerprints of various parts of the black tiger, it can be seen that the detection peaks of black tiger leaves are the most under liquid phase conditions, and the chemical components may be the most complex. )analyze.

[0145] 1.1. Experimental method

[0146] The chemical constituents of black tiger leaves were analyzed by liquid chromatography-mass spectrometry. Pass the alcohol extract of black tiger leaves through a 0.22 μm microporous membrane, and load the sample for LC-MS analysis.

[0147] Chromatographic conditions: using Eclipse Plus C18 chromatographic column (250mm×4.6mm, 5μm), mobile phase is acetonitrile (A)-0.1% formic acid water (B), gradient elution 0~5min, 12%A; 5~10min, 12 %A→20%A; 10~20min, 20%A; ...

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Abstract

The invention belongs to the field of biological medicines, discovers that a kadsura coccinea extract has the effect of inhibiting alpha-glucosidase activity and tyrosinase activity, and a macleaya cordata extract has the effect of inhibiting alpha-glucosidase activity, and particularly provides application of the kadsura coccinea extract and the macleaya cordata extract in preparation of medicines for whitening skin and reducing blood sugar.

Description

[0001] This case is a divisional application, and the application number of the parent case is: 2020105974091 [0002] The application date is: June 28, 2020 The applicant is: Central South University of Forestry and Technology technical field [0003] The invention belongs to the field of cosmetics, and in particular relates to the application of black tiger extract and boluohui extract in preparing whitening products. Background technique [0004] Diabetes mellitus (DM) is a disease caused by the inability of insulin to meet the normal needs of the human body or the resistance of the body to the action of insulin. It is a common endocrine disease. Diabetes mellitus is a metabolic disease with multiple etiologies, characterized by chronic hyperglycemia, sugar, fat, and protein metabolism disorders. The main feature is elevated blood sugar. There are as many as 463 million diabetic patients worldwide, of which type 2 diabetes accounts for about 90%, and there are about 116...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/02A61K36/79A61K36/57A61P3/10A61K127/00
CPCA61K36/79A61K36/57A61P3/10A61K8/9789A61Q19/02A61K2800/805A61K2236/333A61K2236/35A61K2236/31A61K2236/39
Inventor 张胜阳祁斌陈伟波姚烨林
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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