Electrochemical sensor, preparation method and application of electrochemical sensor in detection of ginsenoside Rg3

A ginsenoside and electrochemical technology, applied in the field of analysis and detection, can solve the problems of high instrument maintenance cost, tedious and time-consuming processing, complex equipment, etc., and achieve the effect of good catalytic activity, large electrochemical area and high regeneration ability

Pending Publication Date: 2021-11-09
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, according to the research of the inventors, these methods have disadvantages such as complex equipment, high maintenance costs and tedious and time-consuming sample pretreatment process.

Method used

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  • Electrochemical sensor, preparation method and application of electrochemical sensor in detection of ginsenoside Rg3
  • Electrochemical sensor, preparation method and application of electrochemical sensor in detection of ginsenoside Rg3
  • Electrochemical sensor, preparation method and application of electrochemical sensor in detection of ginsenoside Rg3

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Experimental program
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Embodiment approach

[0050] The third embodiment of the present invention provides an application of the above-mentioned electrochemical sensor in the detection of ginsenoside Rg3.

[0051] The fourth embodiment of the present invention provides a method for detecting ginsenoside Rg3, using the above-mentioned electrochemical sensor as a working electrode, and using potassium ferricyanide as an electrochemically active probe to test the solution containing ginsenoside Rg3 Perform electrochemical detection.

[0052] In some examples of this embodiment, three-electrode electrochemical detection is used.

[0053] In some examples of this embodiment, electrochemical detection is performed using cyclic voltammetry (CV) and / or differential pulse voltammetry (DPV).

[0054] The voltage range of CV detection is -0.4V~0.8V, and the scan rate is 40~60mV s -1 . The voltage range of DPV is from -0.2 to 0.7V, and the scan rate is 40 to 60mV s -1 .

[0055] In order to enable those skilled in the art to un...

Embodiment 1

[0057] Preparation methods of electrochemical sensors, such as figure 1 As shown, the steps are as follows:

[0058] 1. Weigh 1.5mg MWCNT (purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd.) and 0.5mg Ti 3 C 2 T x (purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd.) was dispersed in 1 mL of 0.5wt% PDDA solution. Sonicate for 2 hours, stir for 120 minutes, and then centrifuge and wash with water and ethanol (v / v, 1:1) at 14000 rpm for 4 times, each time for 5 minutes. Discard the supernatant, add an equal amount of water to 1 mL to obtain a uniform nanocomposite suspension, and the nanocomposite is denoted as MWCNT-Ti 3 C 2 T x - PDDA, stored at 4°C for later use.

[0059]2. Pre-polish the bare GCE with 0.3 μm and 0.05 μm alumina powder to obtain a smooth mirror surface, and then use a mixed solution of ultrapure water and ethanol to sonicate for 3 minutes to remove physical adsorption substances. Before modifying the electrode, put the c...

Embodiment 2

[0063] This example is the same as Example 1, except that in step 3, ginsenoside Rg3 is not added in the electropolymerization process to obtain NIP-MWCNT-Ti 3 C 2 T x -PDDA sensor (marked as NIP-MWCNT-Ti 3 C 2 T x -PDDA / GCE).

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Abstract

The invention discloses an electrochemical sensor, a preparation method and application of the electrochemical sensor in detection of ginsenoside Rg3. The electrochemical sensor comprises an electrode, a composite material and a molecularly imprinted polymer are attached to the surface of the electrode, the composite material is a compound of a multi-walled carbon nanotube and titanium carbide, and a template of the molecularly imprinted polymer is ginsenoside Rg3. The preparation method comprises the following steps: adding multi-walled carbon nanotubes and titanium carbide into a solvent containing diethylene glycol diacrylate, compounding to obtain a composite material of the multi-walled carbon nanotubes, the titanium carbide and the phthalate diglycol diacrylate, and modifying the surface of an electrode by adopting the composite material to form a modified electrode; o-phenylenediamine serving as a functional monomer, ginsenoside Rg3 serving as a template molecule, and performing electro-polymerization on the surface of the modified electrode to form a molecularly imprinted polymer, so that the electrochemical sensor is obtained. The electrochemical sensor provided by the invention is used for detecting ginsenoside Rg3 and has the advantages of low cost, fast response, high sensitivity and the like.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and relates to an electrochemical sensor and its preparation method and its application in detecting ginsenoside Rg3. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] With the improvement of people's living standards, people pay more and more attention to the homology of medicines and food. Ginsenoside Rg3 is a rare saponin extracted from the root of red ginseng, which has pharmacological activities such as anti-tumor, hypoglycemic, anti-inflammatory, and anti-oxidative. Studies have shown that it plays an important role in dietary supplementation and disease prevention as an ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 王岱杰杜欣胡金春崔莉赵恒强张浩
Owner SHANDONG ANALYSIS & TEST CENT
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