Curved Surface Microscopic Imaging System and Its Imaging Method

Abstract

The present application relates to the technical field of optical elements, in particular to a curved surface microscopic imaging system and its imaging method. The imaging system includes: a zoom component for adjusting the microscopic imaging focal plane to the corresponding focal plane; During the scanning period of the component, the imaging area corresponding to the focus position is illuminated; the detection light modulation component is used to detect the imaging area and collect the focal plane signals of all planes; the signal synchronization component is used to determine the zoom component, the spatiotemporal illumination modulation component and the detection light modulation The timing relationship between components, and synchronous acquisition timing; the acquisition and image processing component is used to obtain a curved surface image based on the three-dimensional shape distribution of the target and combining the focal plane signals of all planes after the acquisition timing is over. Therefore, high-speed surface modeling and imaging can be realized by adopting time-division multiplexing, fast zoom and spatial light modulation, which ensures high-speed acquisition of surface signals at a certain resolution, and is suitable for time-series imaging of living deep tissues.

Description

technical field [0001] The present application relates to the technical field of optical elements, in particular to a curved surface microscopic imaging system and an imaging method thereof. Background technique [0002] The microscopic imaging system is a bridge connecting the macroscopic and microcosmic worlds. A conventional microscopic imaging system maps a planar sample surface to a planar detector, requiring the detected sample surface to have a strict planar section. The vast majority of samples in nature have complex curved shapes, and the samples need to be sliced ​​for observation under a microscope; however, for living samples, slicing may cause serious damage to the sample. [0003] In related technologies, observing the shape of a curved surface usually requires three-dimensional imaging, which mainly includes the following implementation methods: tomographic technology based on axial scanning, multi-plane detection technology and light field detection technolog...

AI Technical Summary

Problems solved by technology

[0005] The present application provides a curved surface microscopic imaging system and its imaging method, which can realize high-speed curved surface modeling and imaging by adopting time division multiplexing, fast zoom and spatial light modulation, and solve the tomographic technology of axial scanning in the related art Limited by the axial scanning speed and camera imaging speed, the multi-plane detection technology is limited by the size of the camera target surface, the spatial resolution of the light field detection technology is poor, and it takes a long time to ensure the surface signal at a certain resolution High-speed acquisition, suitable for deep tissue time-series imaging in vivo

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