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Application of antibacterial peptide compound DM80Bu20 in preparation of antibacterial toothpaste

A technology of dm80bu20 and dm80bu200.001 is applied in the field of oral care to achieve the effects of long-term storage, reducing gum bleeding and reducing dental calculus

Active Publication Date: 2021-11-23
HAOYIKANG BIOLOGICAL TECH(GUANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is an urgent need to find more new antibacterial peptide active substances, overcome the shortcomings of traditional Chinese herbal toothpaste for non-selective inhibition of various oral pathogens and probiotics, and provide a material basis for the development of oral antibacterial toothpaste products

Method used

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  • Application of antibacterial peptide compound DM80Bu20 in preparation of antibacterial toothpaste
  • Application of antibacterial peptide compound DM80Bu20 in preparation of antibacterial toothpaste
  • Application of antibacterial peptide compound DM80Bu20 in preparation of antibacterial toothpaste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 DM80Bu20 in vitro antibacterial activity test

[0043] 1. Experimental method

[0044] (1) Strain culture

[0045] Candida albicans ATCC SC5314 was cultured in RPMI 1640 medium at 37°C with 80% humidity and 5% CO 2 Incubation under the conditions;

[0046] Staphylococcus aureus ATCC 6538 in TSB medium at 37°C, 5% CO 2 Incubation under the conditions;

[0047] Methicillin-resistant Staphylococcusaureus 18908 in TSB medium, 37°C, 5% CO 2 Incubation under the conditions;

[0048] Porphyromonas gingivalis ATCC 33277 was cultured in BHI medium with hemin (haemin, 5 μg / mL) and vitamin K (menadione, 1 μg / mL) at 37°C under strict anaerobic conditions;

[0049] Streptococcus mutans Clarke Streptococcus mutansClarkeStreptococcus mutans ATCC UA159 in medium Brain Heart Infusion Agar / Broth, 37°C, 5% CO 2 cultivated under conditions;

[0050] Streptococcus sanguis ATCC 10556 in medium Brain HeartInfusionAgar / Broth, 37°C, 5% CO 2 cultivated under conditions.

[00...

Embodiment 2

[0063] Embodiment 2 compound DM80Bu20 hemolytic activity test

[0064] 1. Experimental method

[0065] Fresh human blood was washed three times with Tris buffer (TBS), and the collected human red blood cells (hRBCs) were diluted to 5% (v / v) with TBS. On a 96-well plate, compound DM80Bu20 was diluted to 3.13-400 μg / mL by two-fold serial dilution. After mixing equal volumes of hRBCs suspension and compound DM80Bu20 solution, they were incubated at 37°C for 1 hour. TBS was used as the blank control, and Triton X-100 (TBS) 3.2 μg / ml and hRBCs equal volume mixed solution were used as the positive control. After centrifugation, transfer 80 μl of the supernatant in each well to another 96-well plate, read the OD value of the solution to be tested in the 96-well plate at 405 nm with a microplate reader, and calculate the hemolysis percentage by the following formula:

[0066]

[0067] 2. Experimental results

[0068] Hemolytic activity of DM80Bu20 (HC 50 ) was 200 μg / mL, indic...

Embodiment 3

[0069] Cytotoxic activity test of embodiment 3 compound DM80Bu20

[0070] 1. Experimental method

[0071] Prepare a single cell suspension with DMEM medium containing 10% fetal bovine serum, and the cell density is 1×10 6 CFU / mL, and 1×10 per well 4 Cells were seeded into a 96-well plate with a volume of 100 μL per well. Cells were incubated at 37°C for 24 hours. After the old medium was removed, medium containing different concentrations of compound DM80Bu20 was added, and three replicate wells were set for each concentration. After the cells were cultured at 37° C. for 24 hours, 10 μL of MTT solution (5 mg / mL, prepared in PBS) was added to each well, and the incubation was continued for 4 hours to terminate the culture. Carefully suck off the culture supernatant in the wells, add 150 μL DMSO to each well, and shake on the shaker for 10 minutes to fully dissolve the crystals. On the same 96-well plate, cells treated without any compound DM80Bu20 were included as a contro...

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Abstract

The invention provides application of an antibacterial peptide compound DM80Bu20 in preparation of antibacterial toothpaste and the antibacterial toothpaste prepared from the antibacterial peptide compound DM80Bu20. The research reveals that the antibacterial peptide compound DM80Bu20 can selectively inhibit the activity of most oral pathogens, has no inhibition effect on oral probiotics, has low hemolytic activity and low cytotoxicity, is high in safety and good in stability, and can be used for preparing oral care toothpaste products; furthermore, the antibacterial toothpaste prepared from the compound DM80Bu20 also shows the effect of selectively inhibiting oral pathogens; in addition, the synergistic antibacterial effect of the antibacterial peptide compound DM80Bu20 can be achieved by compounding specific functional components; formation of dental calculi and dental plaque is reduced; gingival bleeding is reduced; decayed teeth and oral ulcers are prevented; halitosis is relieved; and the prepared toothpaste has good antibacterial stability.

Description

technical field [0001] The invention belongs to the technical field of oral care. More specifically, it relates to the application of antibacterial peptide compound DM80Bu20 in the preparation of antibacterial toothpaste. Background technique [0002] Oral health is an important part of overall health. Oral diseases such as caries and periodontal disease will destroy the hard tissues of the teeth and the supporting tissues around the teeth. In addition to affecting the functions of chewing, speech, and aesthetics, they will also cause social difficulties and psychological barriers , and the main cause of these oral diseases is the formation of dental plaque. Effectively solving the formation of dental plaque is the primary task of the research and development of functional oral care toothpaste products. As people pay more attention to dental health problems, nearly 50% of people think that Chinese herbal toothpaste is safer than traditional chemical toothpaste, so Chinese ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/88A61K8/64A61K8/66A61K8/99A61K8/25A61K8/60A61Q11/00A61Q17/00A61P1/02A61P31/02A61P31/04A61P31/10A61P7/04
CPCA61K8/88A61K8/64A61K8/66A61K8/99A61K8/25A61K8/60A61Q11/00A61Q17/005A61P1/02A61P31/02A61P31/04A61P31/10A61P7/04A61K2800/5922Y02A50/30
Inventor 刘润辉褚夫江李毅苹耿帅峰
Owner HAOYIKANG BIOLOGICAL TECH(GUANGZHOU) CO LTD
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