Streptococcus thermophilus for inhibiting helicobacter pylori and application of streptococcus thermophilus

A technology of Streptococcus thermophilus and Lactobacillus acidophilus, applied in the field of applied microorganisms

Pending Publication Date: 2021-12-03
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the resistance o

Method used

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  • Streptococcus thermophilus for inhibiting helicobacter pylori and application of streptococcus thermophilus
  • Streptococcus thermophilus for inhibiting helicobacter pylori and application of streptococcus thermophilus
  • Streptococcus thermophilus for inhibiting helicobacter pylori and application of streptococcus thermophilus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Screening of strains and exploration of biological characteristics

[0036] 1. Strain screening

[0037] Take 10 samples of sauerkraut from a supermarket in Zhengzhou City, Henan Province, each 100.0g, take 5.0-10.0g of sauerkraut samples, put them into a 50.0mL centrifuge tube filled with 20.0mL of distilled water, and shake overnight at room temperature. Take 1.0 mL of the culture solution and make a 10-fold gradient dilution with sterile water to reach 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 , 0.1 mL of each gradient was evenly spread on the MRS plate, and cultured in a 37°C incubator for 48 hours; a suitable gradient growth plate was selected to pick out colonies with different colony morphological characteristics, and after two times of streaking, isolation and purification, 10 primary strains were obtained. Strains R1-R10 were screened.

[0038] The strains obtained from the preliminary screening were inoculated on 0.5% calcium carbonate-MRS solid med...

Embodiment 2

[0059] Embodiment 2 strain identification

[0060] (1) Colony characteristics

[0061] The cell morphology of strain R1 is: long rod-shaped, relatively thin, small colony (diameter about 0.5mm), milky white, raised, rough surface, and curled edges.

[0062] The cell morphology of the strain R9 is: spherical, arranged in chains of varying lengths and lengths, the colonies are off-white, with smooth surfaces and neat edges, and the size of the colonies is about 8mm.

[0063] According to the microscopic observation results of colony characteristics, it was judged that both R1 and R9 were bacteria.

[0064] (2) Molecular features

[0065] Single colonies of R1 and R9 strains were cultured at 37°C overnight, and fresh fermentation broth was taken for DNA extraction and PCR reaction.

[0066] PCR primers were 16S rRNA universal primers 27F and 1492R (27F: 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R: 5'-GGCTACCTTGTTACGACTT-3'). The PCR reaction system was prepared by sterile H 2 22 μL...

Embodiment 3

[0072] Example 3 Optimization of culture conditions

[0073] Fill a 250mL Erlenmeyer flask with 40% volume of MRS medium and adjust the initial pH to 6.2-6.5; after sterilization and cooling, insert 4% Lactobacillus acidophilus LA (R1) or 4% Streptococcus thermophilus ST (R9 ) seeds, after static culture at 37°C for 48 hours, the number of viable bacteria was detected by serial dilution and pouring method.

[0074] (1) Select 33°C, 35°C, 37°C, 39°C and 41°C for the culture temperature, and investigate the influence of the culture temperature on the fermentation. The results are as follows: Figure 6 shown. Depend on Figure 6 It can be seen that temperature has a great influence on the growth of strains in mixed fermentation. When the temperature is in the range of 33°C to 37°C, as the temperature rises, the number of viable bacteria in LA and ST increases gradually. At 37°C, the number of viable bacteria The number reached the highest, respectively 0.26×10 9 cfu / mL and 0....

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Abstract

The invention discloses a newly screened streptococcus thermophilus strain and application of the newly screened streptococcus thermophilus strain to prevention or treatment of helicobacter pylori infection, and belongs to the technical field of applied microbiology. The streptococcus thermophilus strain is preserved in the China General Microbiological Culture Collection Center on July 6, 2020, and has the preservation number being CGMCC No. 20189. The streptococcus thermophilus strain is subjected to mixed culture with lactobacillus acidophilus, and an H.pylori in-vitro antibacterial experiment is carried out through an oxford cup method, the antibacterial effect is judged to be highly sensitive, an obvious antibacterial effect acts on H.pylori, and the strain and studying basis is provided for development of yoghourt and health care products for inhibiting helicobacter pylori.

Description

technical field [0001] The invention relates to a newly screened strain of Streptococcus thermophilus and its application in preventing or treating Helicobacter pylori infection, belonging to the technical field of applied microbes. Background technique [0002] Helicobacter pylori (H. pylori) is a microaerophilic Gram-negative helicobacter that colonizes the surface of human gastric mucosa, which was first reported in 1983. Helicobacter pylori is one of the most common infectious bacteria in the world, and more than half of the population has a past infection. Among them, chronic Helicobacter pylori infection is recognized as one of the most serious risk factors for gastric cancer, and Helicobacter pylori infection is also the main cause of chronic gastritis. In 1994, H. pylori was listed as a class I carcinogen of gastric cancer by the International Agency for Research on Cancer (IARC) of the World Health Organization. [0003] With people's attention to related diseases...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A23L33/135A23C9/12C12R1/46A61K35/744
CPCA61K35/747A61K35/744A61P31/04A23L33/135A23C9/1203A23V2002/00A23V2400/249A61K2300/00A23V2200/30A23V2200/32
Inventor 李永红许福兰李克克冯利菲王蕴王昌葆
Owner ZHENGZHOU UNIV
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