Polysaccharide of aspidopterys obcordata and application thereof

A technology of polysaccharides and vine polysaccharides, applied in the field of plant medicine, can solve the problems of ineffective effect, high recurrence rate, and easy stone-causing components when drinking water is increased.

Active Publication Date: 2021-12-10
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the recurrence rate after treatment is high, generally more than half of the recurrence rate within ten years
At present, the main preventiv

Method used

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  • Polysaccharide of aspidopterys obcordata and application thereof
  • Polysaccharide of aspidopterys obcordata and application thereof
  • Polysaccharide of aspidopterys obcordata and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Grind and sieve, extract 10 times the amount of water at 100°C for 3 times, concentrate, use 5 times the amount of 90% methanol water to disperse the extract, centrifuge (2000rpm / min) for 5 minutes and repeat three times, discard the supernatant , the precipitation accounts for 5%-6%. The precipitation is dissolved in pure water at 50°C. After centrifugation, the supernatant is separated with water as the mobile phase and LH20 gel as the stationary phase. The first column volume fraction is recovered, and the polysaccharide purity is 75%. above.

Embodiment 2

[0047] Grinding and sieving, 12 times the amount of water refluxed at 100°C to extract twice, concentrate, use 5 times the amount of 95% ethanol to disperse the extract, centrifuge (2000rpm / min) for 5 minutes and repeat three times, discard the supernatant, The precipitation accounts for 5%-6%. The precipitation is dissolved in pure water at 50°C. After centrifugation, the supernatant is separated with water as the mobile phase and LH20 gel as the stationary phase. The first column volume fraction is recovered, and the solvent is removed to obtain the polysaccharide.

Embodiment 3

[0049] Grinding and sieving, 10 times the amount of water in a 50°C water bath, ultrasonic extraction for 3 times, concentrated, using 4 times the amount of absolute ethanol to disperse the extract, centrifuging (2000rpm / min) for 5 minutes and repeating three times, discarding Take the supernatant, and take the precipitate to obtain the crude polysaccharide of S.

[0050] Identification of extracted and purified polysaccharides:

[0051] Polysaccharide 1 H-NMR spectrum showed that there was a signal in the abnormal area of ​​δ5.34 (J=3.8Hz), and other signals existed between δ4.01 and 4.15 and δ3.61 and 3.85.

[0052] exist 13 Six main carbon signals were found in the C-NMR spectrum, including the abnormal carbon around δ103.15 and the characteristic peak of sugar ring carbon connected with oxygen in the δ60.30-81.09 region.

[0053] Refer to relevant literature for identification (Li J, Zhang X, Cao L, et al.Three Inulin-Type Fructans from Codonopsis pilosula(Franch.) Nann...

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Abstract

The invention provides aspidopterys obcordata polysaccharide, and experiments find that the polysaccharide has good biological activity, can effectively inhibit formation or growth of calcium oxalate crystals, and can be used for treating kidney stone.

Description

technical field [0001] The present invention relates to the field of plant medicine. Background technique [0002] Kidney stones are one of the common urinary system diseases worldwide, which can cause pain, urinary tract infection, chronic kidney disease, and even loss of kidney function, and its prevalence is increasing year by year worldwide. The types of kidney stones mainly include uric acid stones, calcium stones (calcium oxalate and calcium phosphate stones), phosphate stones and infectious stones, and most of the stones in patients with kidney stones of different ages and regions are calcium stones. Stones are the most common type of kidney stones. Accounts for about 80% of all urinary stones. Only a minority of renal calcium oxalate stones are caused by primary hyperoxaluria, and the cause of renal calcium oxalate stones in most patients remains unclear. For the treatment of kidney stones, the choice of treatment is generally based on the size of the stone. When...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/185A61P13/04A61P13/12A61K135/00
CPCC08B37/0003A61K31/715A61K36/185A61P13/04A61P13/12
Inventor 孙朋许又凯杨绕琼
Owner XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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