Application of Aspidopterys obcordata Hemsl. extract in preparation of medicine for preventing and/or treating diabetic nephropathy

A kind of technology of scutellaria and diabetic nephropathy, applied in the field of biomedicine, can solve the problems of lactic acidosis, hypoglycemia, aggravating the symptoms of metabolic acidosis, etc.

Inactive Publication Date: 2021-04-30
西双版纳傣族自治州人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to lowering blood sugar, the drugs clinically used to treat diabetes do not improve or protect kidney function. Some hypoglycemic drugs even cause kidney damage. For example, biguanides and drugs metabolized by the kidney should not be used when kidney function is damaged. , biguanide hypoglycemic drugs can easily induce lactic acidosis, and can aggravate the symptoms of metabolic acidosis in renal failure; hypoglycemic drugs metabolized by the kidneys can increase the burden on the kidneys, and can cause severe persistent hypoglycemia
Irbesartan is a commonly used drug for the treatment of diabetic nephropathy, and its curative effect is good, but there are indigestion, heartburn, diarrhea, musculoskeletal pain, fatigue and upper respiratory tract infection, anxiety, nervousness, chest pain, pharyngitis, nausea and vomiting, rash, Side effects such as tachycardia
There is no report on the use of this extract in the treatment of diabetic nephropathy

Method used

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  • Application of Aspidopterys obcordata Hemsl. extract in preparation of medicine for preventing and/or treating diabetic nephropathy
  • Application of Aspidopterys obcordata Hemsl. extract in preparation of medicine for preventing and/or treating diabetic nephropathy
  • Application of Aspidopterys obcordata Hemsl. extract in preparation of medicine for preventing and/or treating diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, the extraction of the total steroids of Scutellaria scutellaria

[0048]Get the dry cane 2kg of vine scutellaria, cleanly select, cut into sections, extract twice the combined extract with 95% ethanol (consumption is 3 times the volume of the dry cane of vine scutellaria), the temperature of extraction The temperature is 60°C, and the time is 2h. The extract was concentrated and dried to obtain 247 g of extract. After AB-8 type macroporous resin chromatography, using ethanol-water as the eluent, 60% ethanol eluate was obtained, and after refining, 160 g of the extract of S. chinensis was obtained.

[0049] Taking gentalin A as the reference substance, accurately measure 0.1, 0.2, 0.3, 0.4, 0.5mL of the reference solution, put them in 15mL stoppered test tubes, evaporate to dryness, let it cool, and precisely add the newly prepared vanillin glacial acetic acid solution (Precisely weigh 0.5g of vanillin, add glacial acetic acid to dissolve it into 10mL to ...

Embodiment 2

[0050] Example 2, the protective effect of scutellaria steroids on diabetic nephropathy model mice

[0051] 60 male C57BL / 6N mice, SPF grade, weighing 18-22g, after 5 days of adaptive feeding, 10 were randomly selected as a blank control group, and fed with ordinary rat maintenance feed. The remaining 50 rats were fed with high-fat diet for 4 weeks, fasted but not allowed to drink water for 12 hours, and injected with streptozotocin 150mg / kg to reproduce the type 2 diabetes model. After modeling, blood from the tip of the tail was taken to measure the fasting blood glucose of the mice using a Roche blood glucose meter, and those exceeding 7.8 mmol / L were used as the criterion for successful modeling.

[0052] The mice successfully modeled were randomly divided into positive control group, low-, medium-, and high-dose groups and model group. The positive control group was given 0.04 g / kg diirbesartan by intragastric administration every day, and the low, middle and high dose g...

Embodiment 3

[0058] Example 3, the total steroids of Scutellaria saponifera can inhibit the proliferation of fibroblasts caused by high sugar

[0059] Renal interstitial fibrosis is a key process in the development of diabetic nephropathy. Rat renal fibroblast NRK-49F can accelerate proliferation under higher glucose levels, and is often used as a model for screening anti-fibrotic drugs in vitro.

[0060] NRK-49F cells were cultured in EMEM medium containing 10% fetal bovine serum and 1% double antibody. After digestion, the cells were inoculated into 96-well plates, and after the cells adhered to the wall, different concentrations of total steroids of S. sagittarius were added and cultured for 24 hours. After adding CCK-8 reagent and incubating for 4 hours, the absorbance value was measured in a microplate reader at a wavelength of 450 nm. The absorbance value of the blank control well was regarded as 100% cell survival, and the relative cell survival rate of each group was expressed by p...

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Abstract

The invention provides an application of a Aspidopterys obcordata Hemsl. extract in preparation of a medicine for preventing and / or treating diabetic nephropathy. The medicine for preventing and / or treating diabetic nephropathy provided by the invention takes the Aspidopterys obcordata Hemsl. extract as an active component, and the medicine can be prepared into any dosage form together with pharmaceutically common auxiliary materials. The Aspidopterys obcordata Hemsl. extract can relieve kidney injury and inhibit proliferation of fibroblasts at a high glucose level, has the effect of resisting renal interstitial fibrosis, has no obvious cytotoxicity to normal tissue cells, and has good application prospect in treatment of diabetic nephropathy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the preparation of the [0002] Or the application in the medicine of treating diabetic nephropathy. Background technique [0003] Diabetic nephropathy is one of the chronic and severe microvascular complications caused by diabetes with a relatively high incidence, and it is a major cause of diabetic patients developing end-stage renal disease and death. The clinical symptoms of diabetic nephropathy include proteinuria, glomerular hypertrophy, increased filtration rate, thickened renal basement membrane, and increased number of renal tubules. Tubular atrophy and interstitial fibrosis occur in the later stage. Renal interstitial fibrosis is an important pathological basis for the progressive development of diabetic nephropathy, which can cause tubular atrophy, degeneration, and glomerulosclerosis, eventually leading to renal failure. After 10-20 years of diabetes, about 50...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K45/06A61P13/12A61P3/10
CPCA61K36/185A61K45/06A61K2236/333A61K2236/53A61K2236/55A61P3/10A61P13/12
Inventor 李婷婷杨亚彬
Owner 西双版纳傣族自治州人民医院
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