System including a biological sample treatment chamber
A chamber, cavity technology, applied in the field of systems including biological sample processing chambers
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example 1
[0765] method
[0766] Antigen retrieval time and temperature were manipulated to provide appropriate antigen retrieval for a panel of 14 advanced staining assays.
[0767] This research was made possible by the development of hardware that performs antigen retrieval using a wider temperature range than previously available and without any constraints on the overall time. This allows relatively independent manipulation of time and temperature so that standard times for antigen retrieval can be pursued.
[0768] 5 min was chosen as the total achievable antigen retrieval time, including heating, steady state, and cooling. Temperatures between approximately 100°C and approximately 140°C were initially tested on individual tissues to determine which provided equivalent antigen retrieval to Benchmark ULTRA (Ventana Medical Systems, Inc.; Tucson, AZ). Experiments were performed as a hybrid run where antigen retrieval was performed in a purpose-built reaction chamber and staining...
example 2
[0779]Place the slide in the sealed chamber while approximately 1.6 mL of diluted or concentrated CC1 Demasking Reagent (available from Ventana Medical Systems, Inc., Tucson, AZ) is pipetted across the surface of the slide to completely cover the diagnosable area. Once the test repair reagent is applied to the sample, the sample handling component of the device is turned off and compressed air is introduced into the chamber to increase the pressure to 30 psig. At this point the chamber is heated to expose the sample to a steady state temperature of 125°C. An exemplary temperature profile showing the temperature on a slide during the antigen retrieval process used herein is reproduced in Figure 45 below. In some embodiments, a sample processing assembly for antigen retrieval, such as any of those described herein, takes less than 60 seconds to reach a steady-state temperature of 125° C. Cool the slides for 2 minutes while removing the channels in the chamber bottom assembly. ...
Embodiment 1
[0791] Additional Embodiments 1. A sample processing assembly comprising:
[0792] a subassembly having a first end and a second end;
[0793] a lower plate comprising a lower engagement surface and a raised substrate table relative to at least a portion of the lower engagement surface, wherein the raised substrate table includes an upper surface adapted to hold a substrate horizontally, and wherein the lower plate can be positioned along the subassembly at the second movement in length between one end and a second end; and
[0794] An upper plate including an upper engagement surface and a cavity, wherein the cavity is recessed relative to the upper engagement surface, and wherein the upper engagement surface is complementary to the lower engagement surface.
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