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Pleurotus citrinopileatus polysaccharide and application thereof

A technology of elmia chinensis and polysaccharide, applied in the field of natural product development and utilization, can solve the problems of sarcopenia biological activity, no research report on active ingredients and preparation methods thereof, etc., so as to relieve symptoms of sarcopenia, reduce inflammatory response, increase Effects of body weight and spleen weight

Active Publication Date: 2021-12-24
王琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports on its biological activity, active ingredients and preparation methods for alleviating sarcopenia caused by tumors.

Method used

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  • Pleurotus citrinopileatus polysaccharide and application thereof
  • Pleurotus citrinopileatus polysaccharide and application thereof
  • Pleurotus citrinopileatus polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Step 1, put the fruiting body of the yellow mushroom and deionized water into the wall breaker according to the mass ratio of 1:40, break the wall and extract for 8 minutes under the power condition of 1200W, and obtain the supernatant; at room temperature, the supernatant Concentrate under reduced pressure to the original 20%, and then carry out alcohol precipitation with ethanol with 4 times the volume of the concentrated solution and 80% volume fraction to obtain the crude polysaccharide solution of Pleurotus ulmoides;

[0042] Step 2, add 1 / 4 volume of Sevage solution (by volume ratio, chloroform: n-butanol=4:1) to the crude polysaccharide solution of the yellow mushroom, and fully stir with a magnetic stirrer for 1h (the effect of the Sevage solution is It is used to remove the protein in the polysaccharide solution), after standing for stratification, collect the polysaccharide solution in the upper layer, repeat the operation 3 times, until there is no denatured p...

Embodiment 2

[0044] Step 1. Put the fruiting body of the yellow mushroom and deionized water into a beaker according to the mass ratio of 1:50, and after 40 minutes in a water bath at 90°C, the supernatant is obtained; centrifuged, the precipitate is extracted twice, and the supernatant is combined , concentrated under reduced pressure to the original 20%, and then carried out alcohol precipitation with ethanol with 4 times the volume of the concentrated solution and 80% volume fraction to obtain the crude polysaccharide solution of Pleurotus ulmoides;

[0045] Step 2, add 1 / 4 volume of Sevage solution (by volume ratio, chloroform: n-butanol=4:1) to the crude polysaccharide solution of the yellow mushroom, and fully stir with a magnetic stirrer for 1h (the effect of the Sevage solution is It is used to remove the protein in the polysaccharide solution), after standing for stratification, collect the polysaccharide solution in the upper layer, repeat the operation 3 times, until there is no ...

Embodiment 3

[0047] Step 1, put the fruiting body of the yellow mushroom and deionized water into a beaker according to the mass ratio of 1:40, sonicate at 500W for 16min, then bathe in 90°C water for 2h, and concentrate the supernatant to 20% of its original volume under reduced pressure , and then carry out alcohol precipitation with 4 times the volume of the concentrated solution and 80% ethanol by volume fraction to obtain the crude polysaccharide solution of Pleurotus ulmoides;

[0048] Step 2, add 1 / 4 volume of Sevage solution (by volume ratio, chloroform: n-butanol=4:1) to the crude polysaccharide solution of the yellow mushroom, and fully stir it with a magnetic stirrer for 1h (the effect of the Sevage solution is It is used to remove the protein in the polysaccharide solution), after standing for stratification, collect the polysaccharide solution in the upper layer, and repeat the operation 3 times until there is no denatured protein between the upper and lower layers, and the org...

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Abstract

The invention discloses pleurotus citrinopileatus polysaccharide and application thereof, and relates to the field of development and utilization of natural products. The pleurotus citrinopileatus polysaccharide comprises xylose, glucose, galactose, glucuronic acid, fucose, fructose and arabinose. The invention further discloses application of the pleurotus citrinopileatus polysaccharide in preparation of drugs for treating sarcopenia caused by cancers. A colorectal cancer cachexia mouse muscle attenuation experiment proves that the pleurotus citrinopileatus polysaccharide can increase the body weight and spleen weight of a sarcopenia mouse, recover the form, quantity and density of muscle fibers, reduce inflammatory response and expression of sarcopenia symbolic proteins Atrogin-1 and MuRF-1, and relieve the sarcopenia symptom caused by tumors. According to the pleurotus citrinopileatus polysaccharide and the application thereof, the new effect of the pleurotus citrinopileatus polysaccharide is found, and a new thought is provided for development and utilization of pleurotus citrinopileatus and treatment of the sarcopenia caused by the cancer.

Description

technical field [0001] The invention relates to the field of development and utilization of natural products, in particular to a polysaccharide from yellow fungus and its application. Background technique [0002] Sarcopenia occurs in 50%-80% of tumor patients during the course of the disease. The disease is mainly an inflammatory response, involving multiple organs of the body, and has multiple, devastating and irreversible effects on the body. , seriously affect the quality of life of patients, and even lead to death. In 2016, sarcopenia was listed as a degenerative disease by the World Health Organization together with neurological diseases such as Alzheimer's disease and Parkinson's disease that have received much attention. However, there is currently no effective drug against sarcopenia caused by tumors. [0003] Pleurotus citrinopileatus, also known as Pleurotus aureus, belongs to Basidiomycotina, Phymomycetes, Agaricaceae, Pleurotaceae, and Pleurotus. It is natural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61K31/7004A61K31/7012A61P21/00A61P35/00
CPCA61K36/07A61K31/7004A61K31/7012A61P21/00A61P35/00A61K2300/00Y02A50/30A61K31/715
Inventor 王琦苏玲杨金涛
Owner 王琦
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