Tissue culture technology for cultivating field mutant strain of Wubaiyi Qinan No.1 agilawood

A technique of Wubaiyi and mutant strains is applied in the field of tissue culture for cultivating Wubaiyi Qinan No. 1 Agarwood wild mutant strains, which can solve the problems of the cultivation method being easily affected by the external environment, inconvenient for large-scale cultivation, and lack of cultivation methods. It is easy to imitate, reduce cumbersome steps, and improve the effect of cultivation.

Inactive Publication Date: 2021-12-31
刘志强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a tissue culture technique for cultivating Wubai Yiqinan No. 1 Agarwood field mutant strain, which solves the existing problem of Wubai Yiqinan No. 1 Agarwood field mutant strain proposed in the above-mentioned background technology. Lack of cultivation methods, the cultivation method is easily affected by the external environment, and it is not convenient to carry out large-scale cultivation of the field mutant strain of Wubaiyi Qinan No. 1 Agarwood

Method used

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Examples

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Embodiment example 1

[0020] The present invention provides a technical solution: a tissue culture technique for cultivating Wubaiyi Qinan No. 1 Agarwood field mutant strain, comprising the following steps:

[0021] S1. Take the young leaf tips, leaf stems and rhizomes of Wubaiyi Qinan No. 1 Agarwood, which grow vigorously and are free from diseases and insect pests, as explants, rinse with 5% sodium hypochlorite dilution solution for 3 minutes, then rinse with distilled water for 10 minutes, and soak soak in 10% hydrogen peroxide solution for 15s, then take it out and rinse again with distilled water, place the washed explants in 70% ethanol solution, soak for 12min, then soak in distilled water for 15-30s, take it out and continue to use Rinse with distilled water for 1 min, and dry the moisture on the surface of the explants with medical sterile paper, and set aside;

[0022] S2. Take the leaf tip, leaf stem and rhizome of spare explants, and cross-cut and obliquely cut the leaf tips, leaf stems...

Embodiment example 2

[0026] A tissue culture technique for cultivating Wubaiyi Qinan No. 1 Agarwood mutant in the wild, comprising the following steps:

[0027] S1. Take the young leaf tips, leaf stems and rhizomes of Wubaiyi Qinan No. 1 Agarwood, which grow vigorously and are free from diseases and insect pests, as explants, rinse with 5% sodium hypochlorite dilution solution for 5 minutes, then rinse with distilled water for 15 minutes, and soak Soak in 10% hydrogen peroxide solution for -30s, then take it out and rinse it again with distilled water, put the rinsed explant in 70% ethanol solution, soak for 24min, then put it in distilled water for 30s, take it out and continue to wash it with distilled water Rinse for 3 minutes, and dry the moisture on the surface of the explants with medical sterile paper, and set aside;

[0028] S2. Take the leaf tip, leaf stem and rhizome of spare explants, and cross-cut and obliquely cut the leaf tips, leaf stems and rhizomes of the young explants correspond...

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Abstract

The invention discloses a tissue culture technology for cultivating a field mutant strain of Wubaiyi Qinan No.1 agilawood, and relates to the technical field of cultivation, in particular to the tissue culture technology for cultivating the field mutant strain of Wubaiyi Qinan No.1 agilawood. The technology comprises the following steps of, S1, cutting tender leaf tips, leaf stems and rootstocks of field mutant strains of Wubaiyi Qinan No.1 agilawood as explants, and disinfecting for later use; s2, performing transverse cutting and oblique cutting to form six experimental groups, and comparing and recording callus culture conditions; s3, promoting proliferation of intermediate propagule, and performing redifferentiation to promote rooting and seedling strengthening; and s4, carrying out disinfection and disease and pest resistance cultivation on the rooted seedlings till the transplanting conditions are met, and obtaining the cultivated high-quality field mutant strain of Wubaiyi Qinan No.1 agilawood. According to the technology, the explants are disinfected in advance, the influence of carried bacteria or viruses on cultivation can be effectively reduced, different cultivation groups are matched for mutual contrast, the optimal cultivation condition is determined, and the cultivation effect is improved.

Description

technical field [0001] The invention relates to the field of cultivation technology, in particular to a tissue culture technique for cultivating Wubaiyi Qinan No. 1 Agarwood mutant strain in the field. Background technique [0002] Agarwood is a kind of tree belonging to the family Daphneaceae and the genus Agarwood, with a height of 5-15 meters. The resin accumulated after the old stem is injured is commonly known as agarwood, which can be used as a raw material for spices and a special medicine for treating stomach problems; the bark fiber is flexible, white and delicate, and can be used as a raw material for high-grade paper and artificial cotton; aromatic oil can be extracted from xylem, and flowers can be made extract. [0003] Currently, there is a lack of cultivation methods for Wubai Yiqinan No. 1 wild mutant strain of Agarwood, and the cultivation method is easily affected by the external environment, which is not convenient for large-scale cultivation of Wubai Yiq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘志强
Owner 刘志强
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