The invention discloses a
tissue culture technology for cultivating a field
mutant strain of Wubaiyi Qinan No.1 agilawood, and relates to the technical field of cultivation, in particular to the
tissue culture technology for cultivating the field
mutant strain of Wubaiyi Qinan No.1 agilawood. The technology comprises the following steps of, S1,
cutting tender leaf tips, leaf stems and rootstocks of field
mutant strains of Wubaiyi Qinan No.1 agilawood as explants, and disinfecting for later use; s2, performing transverse
cutting and
oblique cutting to form six experimental groups, and comparing and recording
callus culture conditions; s3, promoting proliferation of intermediate
propagule, and performing redifferentiation to promote rooting and
seedling strengthening; and s4, carrying out disinfection and
disease and pest resistance cultivation on the rooted seedlings till the
transplanting conditions are met, and obtaining the cultivated high-quality field
mutant strain of Wubaiyi Qinan No.1 agilawood. According to the technology, the explants are disinfected in advance, the influence of carried
bacteria or viruses on cultivation can be effectively reduced, different cultivation groups are matched for mutual contrast, the optimal cultivation condition is determined, and the cultivation effect is improved.