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A kind of ultra-low temperature preservation method of tamarind seeds

A cryopreservation and seed technology, applied in the preservation of plants, botany equipment and methods, applications, etc., can solve the problems of affecting seed viability, different cryopreservation conditions, and decreased preservation vigor, so as to reduce respiration intensity and enzyme activity , safe and pollution-free preservation cost, anti-aging effect

Active Publication Date: 2022-07-15
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preservation vigor of Tamarix seeds at room temperature decreases rapidly. Although the technology of cryopreservation of seeds has been applied in many woody plants, the cryopreservation conditions for different seeds are not the same, and even seriously affect the vigor of seeds. Therefore, finding a cryopreservation method suitable for Tamarix seeds is of great significance for the preservation of Tamarix seeds, the establishment of Tamarix germplasm resource bank and the improvement of Tamarix germplasm resource preservation system

Method used

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  • A kind of ultra-low temperature preservation method of tamarind seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The ultra-low temperature preservation is carried out on the seeds of tamarisk short panicle, and the method is as follows:

[0034] Seed collection: During the scattered seed period of Tamarix, select tamarisk plants with robust short spikes, collect well-developed capsules that are about to crack, dry indoors in the shade, and the capsules will naturally spall, and collect fresh seeds and mix them for use.

[0035] Drying: The collected fresh seeds are put into cryopreservation tubes, the mouth of the cryopreservation tube is opened, and it is directly exposed to the air and allowed to dry naturally at room temperature.

[0036] Pre-freezing: seal the mouth of the cryopreservation tube containing the dried seeds, and place it in the freezer of the refrigerator for pre-freezing at -20°C for 2 hours.

[0037] Ultra-low temperature preservation: put the pre-frozen seeds in -80℃ ultra-low temperature refrigerator for 120h.

Embodiment 2

[0039] Other steps and parameters are the same as in Example 1, except that the pre-frozen seeds are put into a -196°C liquid nitrogen tank for 120 hours.

Embodiment 3

[0041] The procedure was the same as in Example 1, except that the seeds on Tamarix longifolia were collected.

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Abstract

The invention belongs to the technical field of germplasm resource preservation, and in particular relates to a method for ultra-low temperature preservation of tamarind seeds. The invention provides a method for ultra-low temperature preservation of Tamarix seeds, comprising the following steps: after pre-freezing the dried Tamarix seeds, store them at -196°C to -80°C. By storing the dried tamarind seeds in the ultra-low temperature, the present invention can make the metabolic activity of the tamarind seeds in a basically stagnant state, thereby prolonging the preservation time of the seeds and ensuring that the seeds maintain strong viability.

Description

technical field [0001] The invention belongs to the technical field of germplasm resource preservation, and in particular relates to a method for ultra-low temperature preservation of tamarind seeds. Background technique [0002] Seeds, as propagules and life carriers of seed plants, contain abundant and sufficient genetic information, are the potential driving force for plant population continuation and vegetation renewal, are an important material basis for genetic improvement and production of fine varieties, and are the best material for long-term preservation of germplasm. . Under natural conditions, the seeds of most plants have a very short lifespan. After leaving the plants, they are stored at room temperature, and their viability plummets. This brings great inconvenience to the work of hybrid breeding and seed production, and is also not conducive to the long-term development of related plant germplasm resources. Protect save. [0003] Tamarix belongs to the genus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 庞新安姜喜焦培培陈家力白宝伟
Owner TARIM UNIV
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