30 results about "Tamarix spp" patented technology

A seed treating agent for promoting the germination of plant seeds, especially the seeds of cistanche, contains gibberelline, naphthylacetic acid, cytokinin, carbendazim, and the optional one of the extracts of three plant roots (tamaisk root, vetchleaf sophora root, etc).

The invention relates to the technical field of afforestation methods of tamarisk hard branches in arid and semi-arid areas, and relates to a method for afforestation of tamarix hard branches in arid and semi-arid areas in summer. The method for afforestation of tamarix summer hardwood cuttings in arid and semi-arid areas in the present invention can significantly improve the survival rate and preservation rate of tamarisk cuttings in summer, and the hard branches of tamarisk can survive in summer cuttings in arid and semi-arid areas. In the present invention , the combination of stored Tamarix hard branches and modern irrigation technology has effectively reduced the adverse effects of limiting factors such as drought, high temperature and sandstorms on Tamarix cutting afforestation, and achieved a breakthrough in time for Tamarix cutting afforestation. In construction, especially in large-scale afforestation and ecological construction, it has great practical significance. In addition, compared with conventional seedling afforestation, the present invention has the advantages of no need to purchase and transport seedlings, no need to dig pits and plant and save The advantage of 80% of the cost of reforestation.

The invention discloses a method for transplanting germinated tamarisk seedlings in saline-alkali land by using salt water. The method is to excavate tamarix seedlings, retain thick roots, trim off all germinated branches and leaves on the top of the seedlings and the trunk to obtain the trunk, and align the roots of the seedlings in the same direction. , binding; soak the bundled seedlings in salt water at the planting point for 24-72 h; dig tree pits, sprinkle diamine phosphate at the bottom of each tree pit, and plant a soaked sapling in each tree pit; plant Then pour the salt water at the planting point; cover the entire tree pit surface with plastic film, compact the plastic film on the edge of the tree pit with soil, and perform secondary watering according to the soil moisture content. The method provided by the invention can not only break the seasonal restrictions on the transplanting of tamarix seedlings, but also significantly reduce the consumption of fresh water, reduce the cost of greening saline-alkali land, and can significantly improve the transplanting survival rate of tamarix seedlings, which is beneficial to the original soil vegetation of coastal saline-alkali land. Construction is important.

The invention discloses a tamarix hispida instantaneous transformation method, and relates to a plant instantaneous transgenosis method. The invention aims at solving the problems that an existing tamarix hispida stable transformation cycle is too long and an original tamarix hispida instantaneous transformation method is low in transformation efficiency. The method comprises the following steps: I, selecting tamarix hispida tissue culture seedlings obtained from subculture, and soaking the selected tamarix hispida tissue culture seedlings in high-permeability treatment liquid; II, rapidly transferring the tamarix hispida tissue culture seedlings which have undergone high-permeability treatment into agrobacterium tumefaciens infection liquid which contains a target gene, conducting vacuumizing in a vacuum pump and conducting infection in a constant-temperature oscillator; and III, taking out the infected tissue culture seedlings, washing the tissue culture seedlings, sucking water, inserting the tissue culture seedlings into a sodium co-culture medium and conducting co-culture. With the application of the method, the instantaneous transformation efficiency of the tamarix hispida is greatly improved. On the basis of an enzyme activity determination result of a marker gene GUS instantaneously transformed gene, it shows that the method, in comparison with the original tamarix hispida instantaneous transformation method, can improve a GUS activity by 2.45 times. The method provided by the invention is applicable to the field of plant instantaneous transgenosis.

The invention relates to a gene coding for a tamarix bristle COL transcription factor and an application thereof, and belongs to the technical field of plant genetic engineering breeding. In order to solve the problem that it is impossible to use the COL transcription factor coding gene of Tamarix brix to breed woody plants with excellent salt tolerance, the invention provides the COL transcription factor coding gene of Tamarix brix and its application. The full length of cDNA of the tamarisk COL gene of the present invention is 1296bp, and the amino acid sequence encoded by the cDNA sequence includes 431 amino acids, which can be used for cultivating salt-tolerant transgenic plant varieties. The overexpression and suppression expression vectors of ThCOL gene were constructed, and the transgenic Tamarix bristles were obtained by transient infection. Through histochemical staining and determination of physiological indicators, it was found that the ThCOL gene overexpression line of Tamarix biribilis had obvious salt tolerance. Therefore, the COL transcription factor coding gene of Tamarix biribilis is an excellent gene for salt-tolerant genetic engineering breeding of forest trees.

The invention relates to a method for greening original soil of heavy saline-alkali land. The method comprises the following steps: digging and filling by a machine to form a tamarisk planting groove, planting tamarisk seedlings in the groove, digging to form a vertical drainage ditch at a certain distance along the direction of the groove, forming a rectangular-ambulatory-plane drainage ditch around the periphery of a planting area, deepening two ends of the vertical drainage ditch into the rectangular-ambulatory-plane drainage ditch area, and building a weir on the outer side of the ditch. According to the method for greening the original soil of the heavy saline-alkali land, the salt elimination engineering is not carried out; the soil is not improved and is just simply dug and filled by the machine; soil salt factors are reduced and discharged by using the terrain advantage according to the soil water salt migration mechanism; the environment suitable for growth of plants is formed; the construction engineering quantity is greatly reduced; the application of the exogenous material is greatly reduced; the construction period is effectively shortened; the cost is low; the maintenance is simple.

The invention relates to a method for preventing and controlling the freeze injury of cistanche tubulosa R.Wight. The method comprises the following steps: inoculating cistanche tubulosa R.Wight in autumn, inoculating in next autumn, digging a heat-preserving gully in the outer side of a position 10-20cm away from an inoculation gully, wherein the heat-preserving gully is an inclined gully inclined from a gully cut and a gully bottom, and the vertical depth of the heat-preserving gully is 50-70cm; and carrying out mechanical punning on the upper side and lateral side, close to the tamarix ramosissima side, of the heat-preserving gully. According to the invention, due to the arrangement of the heat-preserving gully, and according to the position, depth and width of the heat-preserving gully, the solar irradiation area is increased, and the evaporation of soil moisture is accelerated, so that the soil moisture content of a cistanche tubulosa R.Wight inoculation area is reduced, and the soil temperature of the cistanche tubulosa R.Wight inoculation area is increased. With the reduction of the soil moisture content of the cistanche tubulosa R.Wight inoculation area and the increasing of the soil temperature of the cistanche tubulosa R.Wight inoculation area, the depth of a frozen soil layer in winter is reduced, thereby preventing the freeze injury of cistanche tubulosa R.Wight caused by low temperature.

The invention relates to the technical field of hard branch cutting seedling raising, and relates to a method for raising seedlings of tamarix in arid and semi-arid areas in open-air container hard branch cutting in summer; the following steps are carried out: the first step is to select a site; the second step is to purchase a nutrition cup; The third step is to configure the substrate; the fourth step is to place the cup; the fifth step is to make the ear; the sixth step is to soak the cuttings; the seventh step is to irrigate before cutting; the eighth step is to cut; the ninth step is to manage; The tenth step, out of the nursery. The method for cultivating seedlings by hard branch cuttings in open-air containers in arid and semi-arid areas of the present invention has high cutting survival rate and seedling emergence rate, and low seedling raising cost; realizes the purpose of on-site open-air seedling raising in tamarisk summer afforestation areas, provides seedlings, and solves the problem of summer 1. The production and supply of seedlings for off-season afforestation in autumn provides seedling guarantee for Tamarix afforestation using summer flood and autumn slack water, and solves the problem of production, supply and afforestation of off-season seedlings in actual forestry production.

The invention relates to a method for intercropping planting of Cistanche cistanche and Cistanche oleifera in the salinized area of ​​the Yellow River Delta. Under the condition of salinized soil in the Yellow River Delta, Cistanche cistanche is planted between the rows of Tamarix inoculated with Cistanche cistanche to make full use of the tamarisk The land between the rows can increase the coverage of ground vegetation, reduce the evaporation of ground water, inhibit the accumulation of soil salt to the surface, and realize the greening improvement and sustainable use of saline-alkali land.

The present invention relates to a cDNA of Tamarix androssowii ferritin gene and an encoded polypeptide thereof. The invention settles a problem that the growing requirement of plant growth can not be satisfied because of low concentration of iron ions in the dry or half-dry soil. The total length of Tamarix androssowii ferritin gene according to the invention is 1179bp, and the gene sequence of Tamarix androssowii ferritin gene is shown in SEQ ID No.1. The amino acid sequence of ferritin gene is shown in SEQ ID No.2. The ferritin gene can increase the iron ion content of transgenic tobacco for more than 20% when introduced into the tobacco. The gene can increase the utilization efficiency of iron ions by transgenic plant, and the requirement of plant growth is satisfied.

The invention belongs to the technical field of germplasm resource preservation, and in particular relates to a method for ultra-low temperature preservation of tamarind seeds. The invention provides a method for ultra-low temperature preservation of Tamarix seeds, comprising the following steps: after pre-freezing the dried Tamarix seeds, store them at -196°C to -80°C. By storing the dried tamarind seeds in the ultra-low temperature, the present invention can make the metabolic activity of the tamarind seeds in a basically stagnant state, thereby prolonging the preservation time of the seeds and ensuring that the seeds maintain strong viability.

The invention discloses a key gene TcSBP5 regulating tamarix salt tolerance, the nucleotide sequence of which is shown in SEQ ID No.1. The protein product expressed by TcSBP5 is Tamarix SBP transcription factor, and its amino acid sequence is shown in SEQ ID No.2. The invention obtains Arabidopsis thaliana overexpressing the TcSBP5 gene by transforming Arabidopsis thaliana, the salt-tolerant germination rate of its seeds is significantly reduced, the salt-tolerant physiological index of its plants is significantly reduced, and the typical salt-sensitive phenotype of growth inhibition is generally shown , indicating that the gene is an important key factor regulating plant salt tolerance, and has important application value in the field of forest tree salt tolerance breeding.

The invention discloses a method for making integrated seedlings of Cistanche cistanche-Tamarisk. The method involves sowing Cistanche cistanche seeds while cutting the branches of Tamarix. Some of them have no seeds, which can ensure that the early Tamarix root system will not be affected by the inoculation of Cistanche tuberosa seeds, and can effectively prevent the distribution of Cistanche tuberosa inoculation from being too concentrated; The cutting branches survived, which promoted the germination and inoculation of Cistanche tuberosa seeds; the method involved in the present invention has the characteristics of easy operation and easy promotion, and is suitable for directly planting Tamarix-Cistanche tuberosa integrated seedlings in the field, and is also suitable for the public to plant Cistanche tuberoses on balconies And the demand for bonsai is conducive to market promotion.

The invention discloses a tamarisk salt stress response gene TcSBP1 and its miRNA resistance target rTcSBP1 and its application. The nucleotide sequence of the Tamarix salt stress response gene TcSBP1 is shown in SEQ ID No.1, and the amino acid sequence of the expressed protein is shown in SEQ ID No.2. In the present invention, the miR156 response element of the TcSBP1 gene is synonymously mutated by the large primer mutation technology to obtain the miR156 resistance target rTcSBP1, the nucleotide sequence of which is shown in SEQ ID No.3. Under salt stress, TcSBP1 was post-transcriptionally regulated by miR156, while rTcSBP1 was not. The salt stress-responsive gene TcSBP1 of Tamarix and its miR156 resistance target rTcSBP1 are of great value in the research and application of plant salt tolerance or tree resistance breeding.

The invention relates to a method for cultivating Cistanche tubulosa which is applicable to the saline-alkali areas of the Yellow River Delta. The ridges are mainly planted by tamarisk, and the direction of the ridge is north-south. After planting Cistanche tubulosa, the height of the ridge is set to 28-32 cm, and the width of the bottom of the ridge is set to 78 cm. ‑82cm, the width of the top of the ridge is set to 58‑62cm, both sides of the ridge can be effectively irradiated by sunlight, which can effectively avoid the phenomenon of excessive soil moisture and low temperature in winter; prevent the planting of Cistanche tubulosa in the saline-alkali land of the Yellow River Delta from not germinating and It is prone to rot, which improves the yield.

The invention discloses a tissue culture rooting method for Tama Tamarix jintaenia. The tissue culture rooting method comprises the following steps: (1) a current-year twig of Tama Tamarix jintaenia is selected and taken as an explant for rooting culture; (2) the explant is cut into middle sections with the length of 10-15 cm, sediment on the surface is washed away, and then deep cleaning is performed; (3) the explant is subjected to sterilization on an ultra-clean working table; (4) the explant is cut into small sections with the length of 1.5-2.5 cm, and inoculation is performed in a rooting culture medium (1 / 2 MS, IBA 0.4mg / l, and sucrose 25g / l); (5) culture conditions are set: the temperature is 24+ / -2 DEG C, the illumination intensity is 1500-2000 lx, and the illumination time is 10-14 h / d. The method has the beneficial effects that Tama Tamarix jintaenia can root early and rapidly, first rooting occurs after five days, the rooting time is shortened to 15-21 days, the average root length is about 9 cm after 21 days, roots are relatively long, the average quantity of the roots is 5, the quantity of hair roots is large, the rooting percentage is 100%, the upper growth vigor is good, the seedling cost is greatly reduced, and the industrial production of Tama Tamarix jintaenia is realized.

The invention discloses a rhizosphere growth-promoting bacillus arborii in heavy saline-alkali soil and its application. The strain can improve the saline-alkali tolerance of Tamarix in heavy saline-alkali environment. The rhizosphere growth-promoting bacillus arborii of the heavily saline-alkaline soil of the present invention is made into a biological agent, inoculated around the root system of Tamarix, and after a certain period of salt stress, it can promote the growth of Tamarix and improve the growth of Tamarix compared with no inoculation of the agent. Root and plant water content. Therefore, the invention can be used as an inoculant in heavy saline-alkali habitats, and provides excellent strain resources for improving the saline-alkali resistance of Tamarix tamarix.

The invention relates to a recyclable low-cost method for potting shrubs in arid bare ground. The method comprises the following steps: 1), digging and planting holes; 2), assembling potted plants; soil; 4), pruning branches and leaves; 5), fully watering; 7), covering with water-retaining rubber pad. The potting method provided by the present invention is used for the temporary greening project of bare land in arid areas, and the method improves the survival rate of shrubs, and the applicable shrub species include: Ligustrum microphylla, Buxus microphylla, Amorpha fructus, Hibiscus, Tamarix, Bauhinia, and Honeysuckle etc.; potting containers and plants can be relocated as a whole, recycled repeatedly, without post-maintenance management; container components do not need to be customized, low cost, easy to purchase, easy to assemble, and effectively improve the efficiency and effect of bare land treatment projects.

The invention discloses a forest layer in the middle zone of a three-layer ecological sand-preventing and soil-fixing forest belt and a forest forming method thereof. The forest layer is divided into upper and lower levels, the first level is the plant level, and the main body of the level is long spike Tamarix is ​​a flat and long "back"-shaped forest belt composed of main plants. Each main plant has 3-4 kudzu vines planted equidistantly beside the root, and Caragana in the middle is planted outside the area 60cm-70cm away from the main plant; The second level is the supply layer, which is set on the ground 1.5m-2m above the ground, and a layer of starch-grafted sodium acrylate film is laid on the bottom of the supply layer, and a slow-release plant material composed of fallen leaves of the Daxinganling coniferous forest is laid on the film Layer, the slow-release plant material layer is mixed with Rhodobacter vaninii: Bacillus licheniformis: Yeast: Antibiotic Chinella starch-based mixed bacterial powder. The invention has multiple layers, multiple ecological environments, long fertilization period, good sand control effect, strong soil fixation ability and good economical efficiency.

The invention discloses a tamarix salt stress response key gene TcARF6 and its application. The nucleotide sequence of the key gene TcARF6 is shown in SEQ ID NO.1. The expression protein of tamarix salt stress response key gene TcARF6, its amino acid sequence is shown in SEQ ID NO.2. The present invention uses tamarix salt stress treated tamarix as material, and clones TcARF6 gene through RACE technology. Through real-time fluorescent quantitative detection technology, the expression pattern of TcARF6 gene was detected after Tamarix tamarix was stressed, and the key of its response to stress was verified. At the same time, the gateway technology was used to construct the overexpression vector pH35GS‑TcARF6 of Tamarix tamarix. Driven by the promoter P35GS, TcARF6 can be highly expressed in the transgene. The relative quantification of TcARF6 in response to salt stress showed that TcARF6 was only specifically and rapidly down-regulated in roots, which verified the key role of this gene in stress response and has important application value in the field of forest tree resistance breeding.

The invention provides a forest and grass optimal allocation method for the restoration of middle and young degraded shelterbelts in saline-alkali land, and mainly relates to the field of shelterbelt restoration. An optimal allocation method for forest and grass for the restoration of degraded shelter forests in saline-alkali land, which is applied to the restoration of degraded forest stands caused by secondary soil salinization, severe soil failure and other reasons in middle (young) age shelter forests, and is realized by the following steps: land preparation: Before the soil freezes from the end of October to the beginning of November, the trees in the degraded shelterbelt shall be ploughed to prepare the soil. Arbor, shrub and grass configuration: shrubs and grasses shall be planted in the degraded shelterbelt to form a three-dimensional space where trees, shrubs and grasses are mixed. Optimize the configuration structure; shrub and grass planting technology: including shrub Tamarix planting technology and NyPa forage planting technology; management of shrubs and grasses after planting. The beneficial effects of the present invention are as follows: the present invention can effectively reduce soil salinity content, significantly improve soil physical properties, increase soil fertility, promote tree growth, and achieve the purpose of restoring degraded shelter forests.