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A rhizosphere-promoting Bacillus adenii and its application

A technology of Bacillus aeruginosa and rhizosphere growth promotion, applied in the field of agricultural and forestry microorganisms, can solve the problems of reduced salt tolerance, reduced plant rhizosphere biological activity, and no resistance to high salt, so as to promote growth and improve salt and alkali tolerance. ability, the effect of improving the resistance to salinity and alkali

Active Publication Date: 2020-05-22
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most plants and plant rhizosphere growth-promoting bacteria do not have the ability to resist high salinity and high salinity, especially in the case of severe saline-alkali, the beneficial microorganisms in the plant rhizosphere environment decrease, resulting in a decrease in the biological activity of the plant rhizosphere , decreased salt tolerance

Method used

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  • A rhizosphere-promoting Bacillus adenii and its application
  • A rhizosphere-promoting Bacillus adenii and its application
  • A rhizosphere-promoting Bacillus adenii and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening, isolation and preservation of rhizosphere growth-promoting bacteria in heavy saline-alkali soil

[0024] Six soil samples from the rhizosphere of Tamarix tamarisk were collected in the Yellow River Delta. Bacterial isolates were isolated from rhizosphere soil samples by serial dilution method. The isolated bacteria were purified by the three-section line method, and whether the strain was purified was judged by microscopic examination. The purified bacteria were numbered, and single colonies were picked, and inoculated on the skimmed milk powder medium plate with a sterilized toothpick. After culturing at 37°C for 2 days, select colonies with transparent circles, measure the diameter d of the colonies and the diameter D of the transparent circle, and calculate D / d, select the ones with a large ratio for purification and repeatedly verify the effect of protease production, and select strains with stable functions for further Double sieve.

[0025] ...

Embodiment 2

[0026] Example 2 Physiological, biochemical and molecular identification of rhizosphere growth-promoting bacteria GPR018 in heavy saline-alkali soil

[0027] Colony characteristics and cell shape: the colony is round and regular, the colony is large, the surface is raised, relatively moist, slightly yellow, opaque, the cell is small, rod-shaped, with spores, and the colony shape is as follows figure 1 As shown, its optical microscope examination is as follows figure 2 shown.

[0028] Physiological and biochemical characteristics: positive contact enzyme test, positive citrate utilization test, negative hydrolyzed starch test, negative V-P assay test, negative D-glucose test, negative D-mannitol test, negative trehalose test, negative D-sorbitol test , Negative sucrose test, negative glucose gas test.

[0029] Qualitative and quantitative determination of function: Add 1mL of the original fermentation broth to 2mL casein solution with a mass fraction of 0.5%, bathe in water ...

Embodiment 3

[0034] Salt-tolerant ability of embodiment 3GPR018 and the growth-promoting effect under heavy saline-alkali environment

[0035] 1. Salt tolerance

[0036] Select 4 kinds of Bacillus argii, respectively Bacillus arzei BPR078 (preservation number is (CGMCC No.11971), Bacillus arzii BPR088 (preservation number is (CGMCC No. (CGMCC No.11967) and Bacillus arborii GPR018 of the present invention (preservation number is (CGMCC No.11973). The 4 auxin-producing strains were first inoculated into liquid LB medium, 180rpm, 37°C constant temperature shaker culture for 24h Afterwards, take 200 μL of the activated bacterial liquid into the LB liquid culture medium with the concentration of sodium chloride being 1%, 3%, 6%, and 9%, respectively, and each gradient has three parallels, and then place it at 180rpm and shake at a constant temperature of 37°C Bed culture 24h, adopt spectrophotometer, take the corresponding culture medium that does not inoculate bacterial suspension as blank co...

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Abstract

The invention discloses a rhizosphere growth-promoting bacillus arborii in heavy saline-alkali soil and its application. The strain can improve the saline-alkali tolerance of Tamarix in heavy saline-alkali environment. The rhizosphere growth-promoting bacillus arborii of the heavily saline-alkaline soil of the present invention is made into a biological agent, inoculated around the root system of Tamarix, and after a certain period of salt stress, it can promote the growth of Tamarix and improve the growth of Tamarix compared with no inoculation of the agent. Root and plant water content. Therefore, the invention can be used as an inoculant in heavy saline-alkali habitats, and provides excellent strain resources for improving the saline-alkali resistance of Tamarix tamarix.

Description

technical field [0001] The invention belongs to the technical field of agricultural and forestry microorganisms, and in particular relates to a plant rhizosphere growth-promoting Bacillus arborii in heavily saline-alkali land and its application. Background technique [0002] Land salinization is one of the important factors restricting the development of agriculture and forestry. Salinization severely restricts plant growth, thereby having a major impact on crop yields. According to statistics, the loss of crops in irrigated areas due to salinization in the world is as high as 11 billion US dollars, and it is constantly increasing. At present, salinized soil accounts for about 30% of the total land area, and there are more than 1 billion hectares in the world 2 The land is in a state of salinization. According to the statistical report of the second national census organized by the Ministry of Agriculture, the total area of ​​saline soil in China accounts for 4.88% of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 井大炜马海林刘方春刘幸红马丙尧杜振宇杨庆山周健彭琳
Owner SHANDONG FOREST SCI RES INST
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