Tissue culture rooting method for Tama Tamarix jintaenia

Abstract

The invention discloses a tissue culture rooting method for Tama Tamarix jintaenia. The tissue culture rooting method comprises the following steps: (1) a current-year twig of Tama Tamarix jintaenia is selected and taken as an explant for rooting culture; (2) the explant is cut into middle sections with the length of 10-15 cm, sediment on the surface is washed away, and then deep cleaning is performed; (3) the explant is subjected to sterilization on an ultra-clean working table; (4) the explant is cut into small sections with the length of 1.5-2.5 cm, and inoculation is performed in a rooting culture medium (1 / 2 MS, IBA 0.4mg / l, and sucrose 25g / l); (5) culture conditions are set: the temperature is 24+ / -2 DEG C, the illumination intensity is 1500-2000 lx, and the illumination time is 10-14 h / d. The method has the beneficial effects that Tama Tamarix jintaenia can root early and rapidly, first rooting occurs after five days, the rooting time is shortened to 15-21 days, the average root length is about 9 cm after 21 days, roots are relatively long, the average quantity of the roots is 5, the quantity of hair roots is large, the rooting percentage is 100%, the upper growth vigor is good, the seedling cost is greatly reduced, and the industrial production of Tama Tamarix jintaenia is realized.

Description

technical field [0001] The invention relates to a plant rooting method, in particular to a tissue culture rooting method of Tamarix quinta, belonging to the technical field of asexual reproduction. Background technique [0002] Plant tissue culture is a method of asexual reproduction in which the desired tissues, organs or cells, protoplasts, etc. are separated from the plant body, that is, cultured under artificial control conditions through aseptic operation to obtain complete regenerated plants or produce economically valuable Propagation techniques for other products. [0003] In the reproduction process of most seedlings, the traditional sexual reproduction can also achieve the purpose of rooting and rapid propagation, but it is easy to change the original excellent traits of the mother plant during the reproduction process, causing the offspring to mutate. [0004] As a widely used asexual reproduction method at present, tissue culture is not restricted by geographica...

AI Technical Summary

Problems solved by technology

[0006] However, in recent years, due to the harsh and changeable terrain, environment and climate conditions in the distribution area, the natural regeneration of Tamarix jinta has been greatly restricted, and the number of seedlings is decreasing day by day.

At present, researchers mainly adopt the cutting method to propagate the tree, but in the process, there are problems such as difficult preservation of cuttings, low survival rate of cuttings, and slow growth of seedlings after cutting.

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