Palatable extensively hydrolysed whey protein hydrolysates
A technology of whey protein hydrolysis and whey protein, which is applied in the field of new whey protein hydrolyzate, which can solve the problems of whey protein hydrolyzate, such as bitterness, unpleasant taste, unsuitability, etc.
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[0174] The method for preparing whey protein hydrolyzate of the present invention should not be limited to the amount of enzyme added under the hydrolysis step, because the amount of enzyme added depends on the type of enzyme and the activity of the enzyme. However, as a guide, enzymatic hydrolysis is performed with a combination of enzymes, wherein the total amount of enzymes is 0.05-10 g enzyme / 100 g protein, eg 0.1-7.5 g enzyme / 100 g protein. Preferably, the amount of enzyme is in the amount of 0.2-5.0 g / 100 g protein.
[0175] The ratio between the three different enzymes in combinations i to iii may for example be in the range of 1-10:1-10:1-10, for example in the range of 1-8:1-8:1-8. However, the invention should not be limited to the amount of enzyme added, as this will depend on the activity of the enzyme used.
[0176] The enzymatic hydrolysis carried out in step b) of the present invention is preferably carried out at a temperature in the range of 40°C to 75°C, eg ...
Embodiment 1
[0262] Embodiment 1: Analytical method
Embodiment 11
[0263] Embodiment 1.1: the mensuration of degree of hydrolysis (DH)
[0264] The degree of hydrolysis (DH) is defined as the percentage of peptide bonds cleaved by hydrolysis, see equation (1) below. The DH value provides information on the amount of peptide formed, which is related to the number of available peptide bonds.
[0265] DH of whey protein hydrolyzate was measured as described in: Adler-Nissen, J. Determination of the degree of hydrolysis of food protein hydrolysates bytrinitrobenzenesulfonic acid. J. Agric. Food Chem. 27, 1256-1262 (1979). and Nielsen, P.M., Petersen, D. & Dambmann, C. Improved method for determining food protein degree of hydrolysis. J. Food Sci. 66, 642-646 (2001). In equation (1), h represents the number of peptide bonds cleaved, h total Indicates the total number of available peptide bonds. Thus, DH gives the percentage of peptide bonds that are cleaved.
[0266] Equation (1): DH=(Number of free amino termini) / (Total number of available ...
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