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A kind of small molecular RNA that regulates individual division of labor in red import ant workers and its application

A technology of red imported fire ants and worker ants, which is applied in the direction of DNA/RNA fragments, application, recombinant DNA technology, etc., and can solve the problems of unclear function of miRNA in red imported imported imported fire ants

Active Publication Date: 2022-05-10
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many previous research results have preliminarily demonstrated the role of genes and non-coding RNA regulation in individual division of labor in social insects, the identification of miRNAs in RIFA and their functions in mediating individual division of labor in worker ants are still unclear.

Method used

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  • A kind of small molecular RNA that regulates individual division of labor in red import ant workers and its application
  • A kind of small molecular RNA that regulates individual division of labor in red import ant workers and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0017] 1. Set up an artificial ant nest to obtain foraging and rearing worker ants: put the tested insect source into a plastic rearing box (380 mm × 300 mm × 200 mm) for the red imported fire ant nest, and the plastic box serves as the artificial ant nest area , Put the water test tube and food into another plastic box (380mm x 300mm x 200mm), this plastic box is used as a feeding area, and the inner wall is coated with Fluon. A silicone tube with a diameter of 10 mm and a length of 1.0 m was used to connect the artificial ant nest area and the foraging area. Red Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Imported Fire Ant worker ants need to pass through the silicone tube to carry out feeding activities from the "ant nest area" to the "foraging area". After a stable artificial ant road is formed, the worker ants that eat in...

Embodiment 2

[0027] Take several cylindrical plastic boxes (8cm×10cm), which contain a layer of 4cm thick wet sand to maintain humidity, and a layer of 2cm thick gypsum layer on it. The inner wall of the plastic box is coated with Teflon, and put 100 red fires respectively. Ant-foraging worker ants were placed in plastic boxes and starved for 24 hours. Subsequently, the synthetic miR-279c-5p analogue (5'-GACGGGUGUGCUCUAGUUUCA -3') was mixed with 25% blue pigment-containing honey water and fed so that the final concentration was 0.2ug / ul, and the feeding volume 20ul, feeding 4D, changing once a day, repeated 3 nests. At the same time, 25% blue pigment-containing honey water was used as a control. After 96 hours, the brooding behavior of foraging worker ants was tested. Take a 3.5cm plastic petri dish, put a single foraging worker ant into the petri dish and let it stand for 15 minutes, then put one larva or pupa into the same nest, and observe whether the foraging worker ant has brooding ...

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Abstract

The invention discloses a small molecular RNA for regulating the individual division of labor of red imported fire ants and its application. The nucleotide sequence of the RNA is: 5'-GACGGGUGUGCUCUAGUUUCA-3'. The present invention screens and obtains a small molecule RNA-miRNA-279c-5p that regulates the individual division of labor of RIFA workers. After up-regulating the expression of miR-279c-5p, forage-type worker ants produce brooding behavior for RIFA larvae or pupae, The purpose of pest control can be achieved by changing the behavior of worker ants inside and outside the nest. Adding the bait containing miRNA-279c-5p is easier to transfer throughout the ant nest through the mating behavior of social insects, so as to achieve the purpose of pest control.

Description

Technical field: [0001] The invention belongs to the field of biological technology, and in particular relates to a small molecular RNA for regulating the individual division of labor of red imported fire ants and its application. Background technique: [0002] One of the main reasons why RIFA spreads quickly and is prone to outbreaks is that it is a typical social insect because of its special biological characteristics and high degree of social cooperation. The hierarchical differentiation and division of labor of social insects is one of its main characteristics, which are jointly affected by genes and environment (Simpson et al., 2011; Lockett et al., 2016). The division of labor undertaken by workers of RIFA is related to the developmental time. With the time of adult growth and development after worker ants emerge, they can be gradually divided into "nurse" with the behavior of caring for offspring and "nurse" with the behavior of searching and foraging outside the nes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A01N57/16A01P7/04
CPCC12N15/113A01N57/16C12N2310/141
Inventor 陈洁马运杰石庆型齐国君吕利华陈婷
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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