Method for researching effect of microecological preparation on DSS-induced colitis mice

Abstract

The invention relates to a method for researching the effect of a microecological preparation on DSS induced colitis mice, which comprises the following steps: randomly dividing 6-week-old healthy male C57BL / 6J mice into a blank control group, a UC model group and a synbiotics intervention group, carrying out gavage treatment on the mice from two weeks before dextran sodium sulfate modeling to the end of modeling and before sampling, wherein the mice of the control group and the model group are subjected to intragastric administration by sterile water every day, and the synbiotics intervention group is subjected to intragastric administration by synbiotics (six probiotics including lactobacillus reuteri, bifidobacterium lactis, bifidobacterium breve, lactobacillus fermentum, lactobacillus rhamnosus and bifidobacterium animalis, maltooligosaccharide GOS, fructo-oligosaccharide FOS and blueberry extract) every day. The mice freely drink a DSS solution, and it is found that the microecological preparation has the following treatment effects on DSS-induced acute UC mice: the synbiotics preparation can improve intestinal flora disorder of the acute UC mice and effectively relieve colitis and intestinal mucosal barrier injury conditions of the acute UC mice.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a method for the therapeutic effect of probiotics on mice with acute colitis induced by DSS. Background technique [0002] Microecological preparations are live microbial preparations made from normal microorganisms or substances that promote the growth of microorganisms. All preparations that can promote the growth and reproduction of normal microorganisms and inhibit the growth and reproduction of pathogenic bacteria are called microecological preparations. The effect of the Tao can quickly build the intestinal microecological balance, and can prevent and treat diarrhea and constipation no matter in infants, the elderly, or newborn livestock and poultry. [0003] At present, the treatment of ulcerative colitis is mainly to induce and maintain clinical remission and mucosal healing, prevent complications, improve the quality of life of patients, and strengthen the long-term m...

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Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for probiotics to treat DSS-induced acute colitis mice, which has the advantages of improving the intestinal flora disorder of acute UC mice, and solves the problem of drug treatment such as Aminosalicylic acids can reduce the expression of inflammatory signaling factors, but are only effective in mild to moderate patients; cortisol drugs act on immune cells and upper layer cells, and are effective in severe patients, but these drugs have side effects and are prone to formation of Drug dependence; problems with biologic drugs such as infliximab

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