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Lactobacillus paracasei L511 capable of inhibiting growth of liver cancer tumor and application of lactobacillus paracasei L511

A technology of Lactobacillus casei and inhibition of liver cancer, applied in the field of microorganisms, can solve the problems of unclear functions of fermented product components, unclear tumor suppression functions, and unclear strains of probiotics, etc., to improve the structure of intestinal flora and short chain Effects on fatty acid levels, mitigation of colonic inflammation, and strong macrophage polarization

Active Publication Date: 2022-05-27
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, CN105535650A discloses a probiotic composition with anti-tumor function (subcutaneous transplantation of liver cancer cell lines). The strains of probiotics have not been identified, and the formula is not universal due to the significant differences among different strains of the same type of probiotics in terms of probiotic function; similarly, the strains of probiotics in CN10468665 7A and CN101711775A have not been identified, and The function of the components of the fermented product is not clear

Method used

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  • Lactobacillus paracasei L511 capable of inhibiting growth of liver cancer tumor and application of lactobacillus paracasei L511
  • Lactobacillus paracasei L511 capable of inhibiting growth of liver cancer tumor and application of lactobacillus paracasei L511
  • Lactobacillus paracasei L511 capable of inhibiting growth of liver cancer tumor and application of lactobacillus paracasei L511

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation and screening of Lactobacillus paracasei L511: (1) Collect yogurt samples, apply gradient dilutions to MRS solid plates, and cultivate for 24-48h; (2) Select a single colony that conforms to the basic morphology of lactic acid bacteria and carry out streaking on the plate Purify, screen and isolate lactic acid bacteria; (3) culture the above single colony in liquid MRS medium for 24 hours, extract bacterial DNA, perform PCR, and send it to a sequencing company for 16s identification.

[0028] Physiological and biochemical characteristics of Lactobacillus paracasei L511: Gram-positive bacilli, oxidase (-), contact enzyme (-), glycerol (-), inositol (-), inulin (-), erythritol (-) , mannitol (+), raffinose (-), melanotriose (-), sorbitol (+), D-arabinose (-), L-arabinose (-), a-methyl_D- Mannoside (-), starch (-), glycogen (-), a-methyl-D-glucoside (-), D-ribose (+), D-xylose (-), N-acetyl- Glucosamine (+), Xylitol (-), Gentianobiose (-), Amygdalin (+), L-Xylos...

Embodiment 2

[0031] MRS medium preparation: weighed casein peptone 10.0 g, beef extract 10.0 g, yeast extract 5.0 g, glucose 5.0 g, sodium acetate 5.0 g, diamine citrate 2.0 g, Tween 80 1.0 g, dipotassium hydrogen phosphate 2.0 g, magnesium sulfate heptahydrate 0.2 g, manganese sulfate heptahydrate 0.05 g, calcium carbonate 20.0 g, distilled water 1.0 liter, pH 6.8. The bacteria were seeded in MRS liquid medium at a ratio of 1:500, cultured in an anaerobic incubator at 37°C, and the bacterial concentration was determined according to bacterial absorbance (OD600) and bacterial plate count. Cultivate to the end of the logarithmic phase, remove the bacterial liquid, centrifuge at 12000rpm / min for 15-20min, wash with PBS for 2-3 times, and adjust the concentration to 1×10 with PBS 9 CFU / ml was used for gavage in mice.

Embodiment 3

[0032] Example 3 Relief effect of Lactobacillus paracasei L511 on liver cancer in mice

[0033] Forty 15-day-old male C57BL / 6 mice were randomly divided into 4 groups: liver cancer model control group, this strain intervention group, sorafenib treatment group and combined treatment group, with 10 mice in each group.

[0034] Liver cancer chemical model mice were injected intraperitoneally with DEN reagent, 25 mg / kg according to body weight, and CCL4 reagent with a concentration of 20% was intraperitoneally injected once a week, 5ul / g, for 16 weeks from the fourth week of birth.

[0035] During the modeling process, the mice in the chemical model control group and the mice in the sorafenib group were fed 200 ul PBS every two days from the 14th week after birth. Feed 200ul for two days at a concentration of 10 9 CFU / mL of this strain suspension (PBS), the sorafenib group and the combined treatment group were given sorafenib, 30 mg / Kg, for 15 days from the 16th week.

[0036] A...

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Abstract

The invention discloses lactobacillus casei L511 capable of inhibiting growth of liver cancer tumors and application of the lactobacillus casei L511. The lactobacillus casei LSC-L511 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on January 14, 2022, and the preservation number of the lactobacillus casei LSC-L511 is CGMCC (China General Microbiological Culture Collection Center) NO.24309. The lactobacillus casei LSC-L511 is named as Lactobacillus casei LSC-L511. A single strain of the sorafenib has very strong capability of regulating polarization of macrophages, can significantly inhibit the progress of liver cancer, can enhance the treatment effect of sorafenib on liver cancer by inducing ferroptosis of liver cancer cells, and also has multiple functions and applications of relieving colitis, improving intestinal flora structure and short-chain fatty acid level and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and relates to Lactobacillus paracasei L511 capable of inhibiting the growth of liver cancer tumors and uses thereof. Background technique [0002] Hepatocellular carcinoma (HCC) accounts for 90% of the pathological types of primary liver cancer and is the second leading cause of death from malignant tumors in men. In increasing year by year, the situation is more severe. The treatment methods of stage liver cancer mainly include surgery, drugs, radiotherapy and chemotherapy, etc., but the curative effect is poor. The rapid progress and strong metastasis of liver cancer make it difficult to implement surgical treatment. Even if surgical resection is possible, the recurrence rate after five years is as high as 70%. , the five-year survival rate is only 10%, therefore, there is an urgent need for more effective treatment strategies to prolong the survival time and improve the quality of lif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P35/00A61P1/00A61K31/44C12R1/245
CPCC12N1/20A61K35/747A61K31/44A61P35/00A61P1/00A61K2300/00Y02A50/30
Inventor 刘星吟王静静张雪梅季国忠
Owner NANJING MEDICAL UNIV
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