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Application of lactobacillus plantarum L168 in assisting sorafenib in treating liver cancer

A technology of Lactobacillus plantarum and L168, which is applied in the field of Lactobacillus plantarum L168 assisted sorafenib in the treatment of liver cancer, can solve the problems of increased GSH, attenuated ferroptosis, and increased NRF2, so as to relieve colon inflammation, promote ferroptosis, and improve intestinal The effect of the structure of the intestinal flora

Active Publication Date: 2022-06-07
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, Sorafenib will activate the p62-Keap1-NRF2 pathway, resulting in an increase in NRF2, which in turn will lead to an increase in GSH, which will attenuate the occurrence of ferroptosis

Method used

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  • Application of lactobacillus plantarum L168 in assisting sorafenib in treating liver cancer
  • Application of lactobacillus plantarum L168 in assisting sorafenib in treating liver cancer
  • Application of lactobacillus plantarum L168 in assisting sorafenib in treating liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of embodiment 1 Lactobacillus plantarum L168 bacterial liquid

[0023] Prepare MRS medium: weigh casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 2.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0 g, magnesium sulfate heptahydrate 0.2 g, manganese sulfate heptahydrate 0.05 g, calcium carbonate 20.0 g, distilled water 1.0 liter, pH 6.8. The bacteria were seeded in MRS liquid medium at a ratio of 1:500, cultured in an anaerobic incubator at 37°C, and the bacterial concentration was determined according to bacterial absorbance (OD600) and bacterial plate count. Cultivate to the end of the logarithmic phase, remove the bacterial liquid, centrifuge at 12000rpm / min for 15-20min, wash with PBS for 2-3 times, and adjust the concentration to 1×10 with PBS 9 CFU / ml was used for gavage in mice.

Embodiment 2

[0024] Example 2 Lactobacillus plantarum L168 can promote the killing effect of sorafenib on liver cancer

[0025] A mouse model of primary liver cancer was constructed by intraperitoneal injection of DEN and CCL4 into C57BL / 6 mice. During the modeling process, the mice were gavaged with the bacterial solution of the present invention and sorafenib, and the animal body weight, tumor weight and volume were analyzed. The chemical HCC models were randomly divided into 3 groups: liver cancer model control group, sorafenib single treatment group and sorafenib strain combination treatment group of the present invention, each group containing 10 mice.

[0026] Liver cancer chemical model mice were injected intraperitoneally with DEN reagent, 25 mg / kg, according to their body weight on the 14th day of birth. From the fourth week of birth, CCL4 reagent with a concentration of 20% was intraperitoneally injected once a week, 5ul / g, for 16 weeks. During the modeling process, the mice in t...

Embodiment 3

[0029] Example 3 Lactobacillus plantarum L168 can alleviate the side effects of intestinal inflammation caused by sorafenib treatment

[0030] The colorectum of the mice in the liver cancer chemical model group in Example 2 was collected, and the intestinal lengths of the mice in the liver cancer model control group, the Sorafenib single treatment group and the Sorafenib combined treatment group with the strain of the present invention were measured, as shown in the following figure: Figure 4 As shown, compared with the sorafenib treatment group, the intestinal tract of the mice in the combined treatment group showed an increasing trend, and the H&E staining results were as follows Figure 5 It can be clearly seen from the slices that, compared with the liver cancer model control group and the sorafenib single treatment group, the intestinal inflammation of the mice has a tendency to be alleviated after the strain of the present invention is administered orally, Image 6 The ...

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Abstract

The invention discloses application of lactobacillus plantarum L168 in assisting sorafenib in treating liver cancer. The invention relates to application of lactobacillus plantarum L168 combined with sorafenib in preparation of a medicine for treating liver cancer, in particular to application of the lactobacillus plantarum L168 combined with sorafenib in preparation of a medicine for treating liver cancer. The single bacterial strain can assist sorafenib in treating liver cancer, significantly inhibit the progress of liver cancer, relieve side effects such as colitis caused by sorafenib, and have multiple functions and uses of improving intestinal flora structure and short-chain fatty acid level.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the use of Lactobacillus plantarum L168 to assist sorafenib in the treatment of liver cancer. Background technique [0002] Hepatocellular carcinoma (HCC) accounts for 90% of the pathological types of primary liver cancer and is the second leading cause of death in male malignant tumors. In increasing year by year, the situation is more severe. The treatment methods of stage liver cancer mainly include surgery, drugs, radiotherapy and chemotherapy, etc., but the curative effect is poor. The rapid progress and strong metastasis of liver cancer make it difficult to implement surgical treatment. Even if surgical resection is possible, the recurrence rate after five years is as high as 70%. , the five-year survival rate is only 10%, therefore, there is an urgent need for more effective treatment strategies to prolong the survival time and improve the quality of life of patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K31/44A61P35/00
CPCA61K35/747A61K31/44A61P35/00A61K2300/00Y02A50/30
Inventor 刘星吟王静静季国忠张雪梅
Owner NANJING MEDICAL UNIV
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