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Bifidobacterium longum for relieving ulcerative colitis and application thereof

A technology of Bifidobacterium longum and Bifidobacterium longum, applied in the field of microbiology, can solve the problems of reporting the remedial effect of Bifidobacterium longum, and achieve the effects of relieving colon tissue damage, relieving colonic inflammation, preventing or restoring metabolic disorders

Pending Publication Date: 2022-06-24
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most studies on the probiotic effects of bifidobacteria only use animal models, and only focus on the changes in their composition and metabolites in the study of intestinal flora, ignoring the significant differences between human intestinal flora and animal intestinal flora. Potential influence of Fidobacterium antagonizing efficacy in ulcerative colitis
At the same time, studies have reported that the systemic inflammatory state induced by intestinal inflammation can lead to increased complications such as non-alcoholic fatty liver and atherosclerosis (Bessissow T, et al. Incidence and predictors of nonalcoholic fatty liver disease by serum biomarkers inpatients with inflammatory bowel disease[J].Inflamm Bowel Dis,2016,22(8):1937-1944; Fumery M, et al.Thromboembolic events and cardiovascular mortality in inflammatory bowel diseases: a meta-analysis of observational studies[J].JCrohns Colitis, 2014,8:469-479.), but there is no patent literature reporting the relieving effect of Bifidobacterium longum on metabolic disorders associated with colitis

Method used

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  • Bifidobacterium longum for relieving ulcerative colitis and application thereof
  • Bifidobacterium longum for relieving ulcerative colitis and application thereof
  • Bifidobacterium longum for relieving ulcerative colitis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0053] Preparation of liquid medium: prepare MRS medium (such as the product of Qingdao Haibo Biotechnology Co., Ltd.), dissolve it in distilled water, add cysteine ​​hydrochloride 0.5-1g / L, mix well, and then adjust its pH After sterilizing at 6.6-7.0 and 115-121° C. for 15-20 min, the liquid culture medium is obtained.

[0054] Preparation of solid medium: According to the formula of liquid medium, add 1.5-2% agar, mix evenly, then adjust its pH to 6.6-7.0, and sterilize at 115-121°C for 15-20 minutes to obtain the solid medium culture medium.

Embodiment 1

[0055] Example 1: Isolation and screening of Bifidobacterium longum NSP001

[0056] 1. Sample collection

[0057] The fecal samples of healthy people in Nanchang, Jiangxi were collected. The samples were placed in a preservation tube and stored in an incubator with an ice pack. After they were brought back to the laboratory, they were quickly placed in a -80°C refrigerator for separation and screening.

[0058] 2. Isolation and purification of bifidobacteria

[0059] (1) Gradient dilution of fecal sample: In a sterile anaerobic environment, take about 1 g of the fecal sample collected in step 1, add it to 9 mL of normal saline to obtain the first gradient dilution, and draw 1 mL of the first gradient dilution into 9 mL of physiological saline Brine to obtain the second serial dilution, and so on, a total of 9 serial dilutions are prepared;

[0060] (2) Coating culture: draw 100 μL of all the above gradient dilutions and place them on the MRS fixed medium respectively, and af...

Embodiment 2

[0066] Example 2: The effect of Bifidobacterium longum NSP001 on the free radical scavenging function of human cells

[0067] Bifidobacterium longum NSP001 and Bifidobacterium longum ATCC15707 were streaked on the solid medium, and after a single colony grew, they were inoculated into liquid medium, and cultured anaerobically at 37°C for 16-24 hours to reach a stable phase.

[0068] Human colon cancer cells Caco2 were taken and cultured in RPMI 1640 medium for 48 hours at 2 × 10 cells per well. 5 amount is dispensed into a 24-well plate. Add 2 x 10 to each well individually 7 Cfu Bifidobacterium longum NSP001, cultured for 24 hours. After that, the cells cultured from each well were collected, and the level of superoxide dismutase in the cells was determined using an ELISA kit (Nanjing Jiancheng).

[0069] The results showed that Bifidobacterium longum NSP001 could up-regulate SOD ( figure 2 ), the SOD level was increased by 2.44%, which promoted the cell's scavenging fun...

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Abstract

The invention discloses bifidobacterium longum for relieving ulcerative colitis and application of the bifidobacterium longum, and belongs to the technical field of microorganisms. The Bifidobacterium longum NSP001 disclosed by the invention can stimulate human colon cancer cells Caco-2 to secrete superoxide dismutase, and can improve weight loss and DAI indexes of mice with ulcerative colitis, relieve damage to intestinal barriers and tissue structures, regulate secretion of inflammatory factors and oxidative stress level under the conditions of mouse and human intestinal florae, and can be used for preparing the superoxide dismutase for treating the ulcerative colitis and preventing and treating the ulcerative colitis, so that the bifidobacterium longum NSP001 can be used for preparing the superoxide dismutase for treating the ulcerative colitis. The production of intestinal short-chain fatty acid is promoted, the flora function is improved, and the intestinal health is recovered. In addition, the bifidobacterium longum can further prevent or relieve body metabolic disorder accompanied by enteritis. The bifidobacterium longum NSP001 is used for preparing a pharmaceutical composition and fermented food for relieving ulcerative colitis, and has a very wide application prospect.

Description

technical field [0001] The invention relates to a bifidobacteria longum for relieving ulcerative colitis and its application, and belongs to the technical field of microorganisms. Background technique [0002] Ulcerative colitis (UC) is a chronic, idiopathic inflammatory disorder. The lesions are usually located in the mucosa and submucosa of the colon, involving the rectum and sigmoid colon in a continuous distribution. Clinically, persistent blood in the stool, abdominal pain, tenesmus are typical symptoms, and the course of disease is long and difficult to cure. With the increasing incidence in recent years, it has received extensive attention and attention. There are various drugs for the treatment of ulcerative colitis, but traditional drug treatment has many complications, and ulcerative colitis is prone to repeated attacks, which greatly affects the quality of life of patients. [0003] The human gut is an intricate micro-ecosystem, which contains about 100 trillio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L2/84A23L33/135A61K35/745A61P1/04A61P39/06C12R1/01
CPCC12N1/20A23C9/1234A23L2/84A23L33/135A61K35/745A61P1/04A61P39/06A23V2002/00A23V2400/533A23V2200/3204A23V2200/30Y02A50/30
Inventor 聂少平谭惠子陈梦麟姚虹陈晓敏吴泉勇兰天
Owner NANCHANG UNIV
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