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SiPLATZ12 gene for regulating growth and development of millet and application of SiPLATZ12 gene

A growth and development, millet technology, applied in application, genetic engineering, plant genetic improvement and other directions, to achieve the effect of increasing yield per plant, increasing ear weight, and increasing 1000-grain weight

Active Publication Date: 2022-02-01
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no members of the PLATZ family in millet have been reported yet.

Method used

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  • SiPLATZ12 gene for regulating growth and development of millet and application of SiPLATZ12 gene
  • SiPLATZ12 gene for regulating growth and development of millet and application of SiPLATZ12 gene
  • SiPLATZ12 gene for regulating growth and development of millet and application of SiPLATZ12 gene

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Obtaining of SiPLATZ12 transgenic strain

[0031] 1. Sequence analysis, cloning and vector construction of the millet SiPLATZ12 gene:

[0032] The present invention uses the millet genome database website phytozome to find the gene SiPLATZ12, and the NCBI analysis shows that this gene, like all members of the PLATZ protein family, has a conserved zinc finger domain. The full length of the SiPLATZ12 genome sequence is 1827bp (the sequence is shown in SEQ ID NO.1), the CDS sequence is 756bp (the sequence is shown in SEQ ID NO.2), and the sequence of the PLATZ12 protein encoded by it is shown in SEQ ID NO.3 Show.

[0033] According to the CDS sequence of the SiPLATZ12 gene, primers were designed and cloned. The cloning method is as follows:

[0034] (1) Extraction of RNA: The total RNA of millet was extracted using Biotec Universal Plant Total RNA Extraction Kit.

[0035] (2) Synthesis of the first strand of reverse-transcribed cDNA: the extracted RNA was ...

Embodiment 2

[0049] Example 2: SiPLATZ12 affects plant growth and development

[0050] 1. Developmental phenotypes of transgenic lines overexpressing SiPLATZ12

[0051] 1. The wild-type millet Ci846 was used as a control, and the SiPLATZ12 gene expression level was detected on the obtained homozygous transgenic material 35S::SiPLATZ12. The result is as image 3 As shown, compared with the wild-type Ci846, the expression levels of the SiPLATZ12 gene in the transgenic material 35S::SiPLATZ12#22, #24 and #28 were all significantly increased, which proves that the present invention has indeed obtained the SiPLATZ12-overexpressed millet transgenic lines.

[0052] 2. The millet transgenic line 35S::SiPLATZ12 and the wild type millet Ci846 were cultivated under the same conditions. The seedlings, plant height, stem width, ear position and stem length of the transgenic line 35S::SiPLATZ12 and wild-type Ci846 during growth and development were observed and counted. The results are as follows Fi...

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Abstract

The invention discloses a SiPLATZ12 gene for regulating and controlling growth and development of millet and application of the SiPLATZ12 gene. The gene SiPLATZ12 with the nucleotide sequence shown as SEQ ID NO.1 is separated and cloned from foxtail millet, after the gene is over-expressed in the foxtail millet, a transgenic plant with the plant height being shortened, the stem being thickened and the ears and the grains being remarkably increased is obtained, and the specific expression is that compared with a wild type plant, the plant line of the over-expression SiPLATZ12 gene has the advantages that the plant height and the stem length of the ears of the foxtail millet are remarkably reduced, and the stem width of the millet, the ear length of a single ear, the ear width, the single ear yield, the grain length, the grain width and the thousand grain weight are obviously increased, so that the effect of promoting the development of the millet ear and the grain and improving the millet yield can be realized. Therefore, the SiPLATZ12 gene has important theoretical and practical significance for improving the yield of millet, plays an important role in improving high-yield varieties of crops, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a SiPLATZ12 gene for regulating the growth and development of millet and its application. Background technique [0002] Millet belongs to the genus Setaria of the Poaceae Panicaceae, and is known as "the first of the five grains" and is an important food crop. Millet is rich in nutrition, its protein content is high, the grain contains 17 kinds of amino acids, unsaturated fatty acids account for about 85%, and is rich in vitamins A, B1, B2 and E, iron, magnesium, selenium and other trace elements. Millet is a self-pollinated diploid C4 plant with a small genome, short growth cycle, and many ears of fruit. At present, multiple sets of reference genomes have been obtained; It is a model crop for the study of functional genes of subfamily crops, and is an excellent research material for the study of C4 photosynthesis and stress response of Gramineae crops. [0003] PLATZ i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/10A01H6/46
CPCC07K14/415C12N15/8261
Inventor 吴长艾肖圣慧范佳音郑成超
Owner SHANDONG AGRICULTURAL UNIVERSITY
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