Bioluminescence multiband stereo imaging system for living small animal

Abstract

The invention provides a bioluminescence multiband stereo imaging system for a living small animal. The bioluminescence multiband stereo imaging system comprises an illumination light source group, a spectroscope, a first camera, a second camera, a first reflector and a second reflector, wherein the first reflector and the second reflector are symmetrically arranged on a transparent flat plate; the illumination light source group comprises at least three illumination light sources, the first illumination light source is located below the transparent flat plate, and the second illumination light source and the third illumination light source are arranged on the two sides above the transparent flat plate; the first camera and the spectroscope are located above the transparent flat plate; an included angle exists between the spectroscope and the first camera, so that one part of an imaging light beam enters the first camera, and the other part enters the second camera; and the first reflector and the second reflector are used for reflecting imaging light beams from the second illumination light source and the third illumination light source. According to the bioluminescence multiband stereo imaging system for the living small animal of the invention, omnibearing illumination stereo observation can be realized, and high-precision spatial stereo positioning of a signal target spot is realized.

Description

technical field [0001] The invention belongs to the technical field of medicine and biological imaging, and in particular relates to a small animal live bioluminescent multi-band stereoscopic imaging system. Background technique [0002] The optical imaging technology of living small animals has a wide application potential in the research of bioluminescence, drug enrichment metabolism, cell fluorescent labeling, and fluorescent biological dyes. Optical imaging has the characteristics of high resolution, non-invasive, real-time, and fast feedback, and is suitable for long-term dynamic observation. [0003] Luminescence imaging in vivo mainly uses visible light and near-infrared bands. The fluorescent protein and fluorochrome approach requires excitation light of a specific wavelength, so active illumination is required. Luciferase, on the other hand, oxidizes specific targeted tumor cells to emit light without the need for excitation light. Different luciferases emit spec...

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Problems solved by technology

[0004] However, in the prior art, the upper lighting can only obtain an image of a viewing angle by exciting a limited area depth, and cannot obtain the three-dimensional orientation of the target.

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