Multiplex PCR chip and multiplex-PCR detection method using the same

Abstract

A multiplex PCR chip capable of simultaneously detecting multiple target genes and a multiplex PCR method using the same are proposed. More specifically, the multiplex PCR chip and multiplex PCR method are provided, after a plurality of spatially separated particle-forming grooves is formed in one or more reaction chambers and a probe in a solution state is injected into the particle-forming grooves, planar shapes of the particle-forming grooves are varied or shapes and patterns of particle holders respectively formed on inner surfaces of the particle-forming grooves are varied, and the probe including primers specifically hybridizing with sequences of different nucleic acid molecules is injected into the particle-forming grooves, whereby simultaneous multiplex detection is possible by allowing multiple target genes to be detected on the basis of positions and shapes of the probe particles and the shapes and patterns of the particle holders respectively formed inside of the probe particles.

Description

technical field [0001] The invention relates to a multiple PCR chip capable of simultaneously detecting multiple target genes and a multiple PCR method using the same. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction; PCR) is a method of repeatedly heating and cooling a sample solution including nucleic acid molecules to replicate in a chain and amplify a site (target nucleic acid) having a specific nucleotide sequence geometrically The technology is a technology that can detect target genes by amplifying and replicating a small amount of nucleic acid. [0003] Such a PCR method repeatedly executes a DNA denaturing step, an annealing step, and a DNA synthesis step. The DNA denaturing step (denaturing step) is to heat a sample solution containing a double-stranded template DNA at a specific temperature, for example, at about 95° C. for 5 seconds to The step of separating double-stranded DNA into single-stranded DNA; the annealing step (annea...

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Problems solved by technology

[0008] However, there is a problem in conventional multiplex PCR chips that when spatially separated probes are immobilized on the reaction chamber, that is, when a few drops of probes in a liquid state including primers are dropped to fix them, the probes Difficult to form decent probe particles due to droplet diffusion

In addition, there is a problem that the probe particles are easy to drop when forming the probe particles

In addition, there is a problem that the number of target genes that can be detected is limited due to the limitation of obtaining various combinations only by the position of the probe, and there is also a problem that the position of the probe changes when the left and right sides of the PCR chip change. , so it is difficult to limit the problem of simultaneous multiple detection

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