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Anti-decomposition agent for oxidative metabolite of bilirubin

An anti-decomposition and bilirubin technology is applied in the field of anti-decomposition agents for bilirubin oxidative metabolites, which can solve the problems of difficulty in transportation and storage, inability to measure accurate concentrations, and rapid decomposition of bilirubin oxidative metabolites, etc. The effect of preventing decomposition

Pending Publication Date: 2022-03-01
锐思沃株式会社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the oxidized metabolites of bilirubin have the problems of rapid decomposition, difficulty in transportation and storage
In fact, even when urine is collected from a patient, oxidized metabolites of bilirubin are decomposed during transportation and / or storage at the examination center, and its exact concentration cannot be measured
Therefore, diagnostic methods using bilirubin oxidation metabolites in vivo as markers have not yet been practically used

Method used

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  • Anti-decomposition agent for oxidative metabolite of bilirubin
  • Anti-decomposition agent for oxidative metabolite of bilirubin
  • Anti-decomposition agent for oxidative metabolite of bilirubin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135] (Example 1: Research on anti-decomposition agent of bilirubin oxidative metabolites)

[0136] Antioxidant substances were added to urine to investigate whether the decomposition of oxidized metabolites of bilirubin in urine was inhibited. As antioxidant substances, ascorbic acid (Fujifilm Wako Pure Chemical Industries, Ltd.), sodium chloride (Fujifilm Wako Pure Chemical Industries, Ltd.), reduced glutathione (NACALAI TESQUE, Inc.), L-cysteine ​​( Fujifilm Wako Pure Chemical Industries, Ltd.), riboflavin (Fujifilm Wako Pure Chemical Industries, Ltd.), and peroxidase (Fujifilm Wako Pure Chemical Industries, Ltd.).

[0137] Urine was collected from humans (N=4), and antioxidant substances were added and incubated at 27°C for 48 hours. Then, the concentration of bilirubin oxidation metabolites in each sample was measured. As a standard sample, a sample stored at -80° C. without adding an antioxidant substance to urine collected from a human was prepared. The concentratio...

Embodiment 2

[0142] (Example 2: Time-dependent changes in the concentration of oxidized metabolites of bilirubin in urine under a severe temperature environment)

[0143] This experiment investigated whether the anti-decomposition agent of the present invention can inhibit the decomposition of bilirubin oxidative metabolites in urine even in a severe temperature environment (about 28°C to about 32°C) in midsummer.

[0144] Urine was collected from 3 healthy persons (2 males and 1 female), and the urine samples were preserved for 5 days under the following conditions (1) to (3). As a standard sample, a sample stored at -80° C. without adding an antioxidant substance to urine collected from a human was prepared. The concentration of bilirubin oxidation metabolites in each urine sample was measured at 24 hours, 48 ​​hours, 72 hours, 96 hours and 120 hours after the start of the experiment. This measurement was performed using a bilirubin oxidation metabolite ELISA kit (Metallogenic).

[014...

Embodiment 3

[0153] (Example 3: Time-dependent changes in oxidized metabolites of bilirubin in urine under low temperature conditions)

[0154] This experiment investigated to what extent the anti-decomposition agent of the present invention can inhibit the decomposition of bilirubin oxidative metabolites in urine under low temperature conditions (about 4°C).

[0155]The same urine sample as in Example 2 was used. Ascorbic acid was added (final concentration 10 mM) and stored at 4°C. The concentrations of bilirubin oxidative metabolites in the urine samples were measured at 24 hours, 48 ​​hours, 72 hours, 96 hours and 120 hours after the start of the experiment. This measurement was carried out using a bilirubin oxidation metabolite ELISA kit (Metallogenic).

[0156] The results are shown in Table 3 and figure 2 . The concentration of oxidized metabolites of bilirubin did not change at all when stored at -80°C. This result shows that when 10 mM ascorbic acid is used and stored at 4° ...

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Abstract

The present inventors found that an antioxidant substance having an enediol structure can prevent decomposition of a bilirubin oxidative metabolite. Therefore, the present invention provides an anti-decomposition agent for a bilirubin oxidative metabolite, which contains an antioxidant substance having an enediol structure, or a salt, ester, or glycoside thereof, or a solvate, stereoisomer, or tautomer thereof. A biological sample to which this decomposition inhibitor is added can inhibit the decomposition of bilirubin oxidative metabolites over a long period of time at ambient temperature or below.

Description

technical field [0001] The present invention relates to an anti-decomposition agent for oxidized metabolites of bilirubin, a biological sample to which an anti-decomposition agent for oxidized metabolites of bilirubin is added, and a method for using an anti-decomposition agent for oxidized metabolites of bilirubin Anti-decomposition agent for oxidized metabolites of erythrin, method for preventing decomposition of oxidized metabolites of bilirubin in biological samples, preservation method of biological samples, effect on oxidized metabolites of bilirubin in biological samples methods for the determination of concentrations, and methods for assessing the state of oxidative stress. Background technique [0002] Bilirubin oxidative metabolites are oxidative decomposition products of bilirubin reacting with active oxygen in vivo. It has been suggested that bilirubin oxidative metabolites in vivo are useful as markers of oxidative stress and mental illness (Non-Patent Document...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K15/06G01N33/72
CPCG01N33/72C07D307/62C09K15/06
Inventor 大西新
Owner 锐思沃株式会社