Whitening collagen peptide freeze-dried powder and preparation method thereof

A technology of white collagen and freeze-dried powder, which is applied in the preparation method of peptide, freeze-dried transportation, peptide/protein composition, etc., can solve the problem of lack of whitening collagen peptide products, and achieve easy operation, high whitening activity, and advanced technology simple effect

Pending Publication Date: 2022-03-04
天津知了大健康产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the lack of existing whitening collagen peptide products, the present invention provides a whitening collagen peptide freeze-dried powder, the tyrosinase inhibitory activity of the collagen peptide freeze-dried powder can reach more than 50%, and can reduce melanin The melanin content of tumor cells is more than 40%

Method used

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  • Whitening collagen peptide freeze-dried powder and preparation method thereof
  • Whitening collagen peptide freeze-dried powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of whitening collagen peptide freeze-dried powder, adopts the following steps:

[0025] (1) Raw material preparation: add 100g of pigskin collagen into water, adjust the pH to 3.0 with acetic acid and stir to prepare a solution;

[0026] (2) One-time enzymatic hydrolysis: add 0.15g pepsin and 0.15g papain to the solution in step (1), adjust the pH to 1.5 and stir, control the temperature at 30°C for 0.5 hours, inactivate the enzyme, and adjust the pH to neutral ;

[0027] (3) Membrane separation: the enzymolysis solution obtained in step (2) is ultrafiltration treated with an ultrafiltration membrane with a molecular weight cut-off of 1500Da, and the retentate is collected;

[0028] (4) Secondary enzymatic hydrolysis: add 0.75 g of chymotrypsin complex protease and 0.25 g of alkaline protease to the retentate obtained in step (3), adjust the pH to 7.5 and stir, and enzymolyze at a temperature of 30° C. for 1 hour to inactivate the enzyme;

[0029]...

Embodiment 2

[0032] A preparation method of whitening collagen peptide freeze-dried powder, adopts the following steps:

[0033] (1) Raw material preparation: add 100g of bovine bone collagen into water, adjust the pH to 4.0 with acetic acid and stir to prepare a solution;

[0034] (2) One-time enzymatic hydrolysis: add 0.2g pepsin and 0.4g papain to the solution in step (1), adjust the pH to 2.5 and stir, control the temperature at 40°C for 2 hours, inactivate the enzyme, and adjust the pH to neutral ;

[0035] (3) Membrane separation: the enzymolysis solution obtained in step (2) is ultrafiltration treated with an ultrafiltration membrane with a molecular weight cut-off of 1500Da, and the retentate is collected;

[0036] (4) Secondary enzymatic hydrolysis: add 1.5 g of chymotrypsin complex protease and 0.5 g of alkaline protease to the retentate obtained in step (3), adjust the pH to 8.5 and stir, and enzymolyze at a temperature of 40° C. for 3 hours to inactivate the enzyme;

[0037] ...

Embodiment 3

[0040] A preparation method of whitening collagen peptide freeze-dried powder, adopts the following steps:

[0041] (1) Raw material preparation: add 100g of fish scale collagen into water, adjust the pH to 3.5 with acetic acid and stir to prepare the solution;

[0042] (2) Enzymolysis once: add 0.35g pepsin and 0.35g papain to the solution in step (1), adjust the pH to 2.0 and stir, control the temperature at 35°C for 1 hour, inactivate the enzyme, and adjust the pH to neutral ;

[0043] (3) Membrane separation: the enzymolysis solution obtained in step (2) is ultrafiltration treated with an ultrafiltration membrane with a molecular weight cut-off of 1500Da, and the retentate is collected;

[0044] (4) Secondary enzymatic hydrolysis: add 1.0 g of chymotrypsin complex protease and 0.5 g of alkaline protease to the retentate obtained in step (3), adjust the pH to 8.0 and stir, and enzymolyze at a temperature of 35° C. for 2 hours to inactivate the enzyme;

[0045] (5) Vacuum ...

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Abstract

The invention belongs to the field of skin-care external medicines, and particularly relates to whitening collagen peptide freeze-dried powder and a preparation method thereof. The content of protein in the whitening collagen peptide freeze-dried powder is greater than or equal to 95 wt.%; wherein the content of protein peptide with the molecular weight of 500-1000 in the protein is greater than or equal to 95 wt.%; the content of short peptides containing tyrosine residues in the protein peptide is greater than or equal to 50wt.%. According to the whitening collagen peptide freeze-dried powder provided by the invention, the tyrosinase inhibitory activity of the collagen peptide freeze-dried powder can reach more than 50%, and the melanin content of melanoma cells can be reduced by more than 40%.

Description

technical field [0001] The invention belongs to the field of external skin care medicines, and in particular relates to a whitening collagen peptide freeze-dried powder and a preparation method thereof. Background technique [0002] The pigmentation process of human skin is very important. It not only determines the color of skin, eyes and hair, but also is the first barrier to protect the skin from the damage of ultraviolet radiation. However, melanin metabolism disorders can cause pigmentation diseases such as spots, freckles, chloasma and melanoma. The metabolic process of melanin includes the production of melanin, the transport of melanin from melanocytes to keratinocytes, and the distribution and degradation of melanin in keratinocytes. The simple process is as follows: First, under the regulation of genes, the structure of melanosomes is formed. After tyrosinase is synthesized in the endoplasmic reticulum, it arrives at the Golgi apparatus for saccharification and pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/39A61K9/19A61P17/00A61K8/65A61K8/02A61Q19/02C07K14/78C07K1/34C12P21/06
CPCA61K38/39A61K9/19A61P17/00A61K8/65A61K8/022A61Q19/02C07K14/78C12P21/06A61K2800/782A61K2800/84A61K2800/805
Inventor 姚亚伟康跻耀孔英俊
Owner 天津知了大健康产业发展有限公司
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