Fermentation process for culturing schizochytrium limacinum with high grease content

Abstract

The invention belongs to the field of microbial fermentation, and particularly relates to a production process for improving the grease yield of schizochytrium limacinum by changing nutrient components of a culture medium of schizochytrium limacinum. Comprising the following steps: 1) culturing a seed solution; 2) performing initial fermentation: performing initial fermentation culture on the seed culture solution; 3) performing single factor analysis: performing culture analysis on carbon sources, nitrogen sources and inoculum sizes with different concentrations of the schizochytrium limacinum through the single factor analysis so as to determine an optimized concentration determination amount; 4) orthogonal experiment: performing orthogonal experiment on the optimized carbon source, nitrogen source and exogenous additives to determine optimal schizochytrium limacinum culture medium components; (5) performing shake flask fermentation: performing shake flask fermentation according to the optimal fermentation parameters obtained in the steps (3) and (4); (6) collecting thalli: performing centrifugation and drying on the bacterial liquid obtained in the step (5) in sequence to obtain schizochytrium limacinum bacterial powder. The method is low in cost, and the biomass and the grease content of schizochytrium limacinum fermentation are high.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a production process for improving the oil yield of Schizochytrium by changing the nutrient composition of the culture medium of Schizochytrium. Background technique [0002] Schizochytrium (Scfizochytrium), also known as Schizochytrium, is a heterotrophic microalgae that lacks chlorophyll and cannot perform photosynthesis. The growth rate of Schizochytrium is fast, and a large amount of bacteria can be produced in 3 to 7 days; the oil content in the cells is high, generally accounting for 40% to 70% of the dry weight of the bacteria; the oil components are relatively simple, and the DHA content is 20% % to 50%, and more than 90% of the fatty acids produced are in the form of triglycerides that are easily absorbed by the human body. In recent years, studies have also found that in addition to DHA, Schizochytrium can also produce EPA, horncrane, carotenoids and o...

AI Technical Summary

Problems solved by technology

Among them, medium optimization and culture condition control are two important factors in the fermentation process of industrial microorganisms. At present, there are few studies on the medium optimization and culture condition control of Schizochytrium, which urgently need to be strengthened.

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