Fermentation process for culturing schizochytrium limacinum with high grease content
A technology of Schizochytrium and fermentation process, which is applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., to achieve the effects of simple extraction process, increased biomass and oil content, and wide application prospects
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[0035] Example
[0036] 1. In order to determine this experiment Schizochytrium species were grown, and the growth of the biomass optimal conditions for lipid accumulation, has been explored in carbon source, nitrogen source, inoculum size by single factor experiments.
[0037] (1) Medium composition:
[0038] Seed Medium (g / L): glucose 30, yeast extract 4, 4 peptone, natural sea salt 20PH value, high temperature sterilization at 120 deg.] C 20min. .
[0039] The initial fermentation medium (g / L): glucose 80, yeast extract 4, 4 peptone, natural sea salt 20PH value, high temperature sterilization at 120 deg.] C 20min. .
[0040] (2) Culture conditions: inoculum: 5% rotational speed: 200r / min Temperature: 25 ℃ seed medium: 2 days of fermentation: 4 days
[0041] (3) multi-laboratory strain is deposited using a liquid medium at -80 ℃ for deposited, a deposited tube glycerol, glycerol ratio of 20% ~ 40%, - 20 ℃ refrigerator can be preserved for about 2 months, -80 ℃ for It can b...
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