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Regeneration culture method of saussurea involucrata

A technique of regenerative culture and rooting medium, which is applied in the field of plant cultivation, can solve the problems of low seed germination rate, high seedling mortality, and harsh growth conditions of Tianshan Snow Lotus, and achieve the effects of saving manpower, shortening breeding time, and promoting germination

Pending Publication Date: 2022-03-15
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] Due to the harsh growth conditions of Tianshan Snow Lotus, the extremely low self-renewal ability of the population and the over-harvesting of people, the wild resources of Tianshan Snow Lotus are seriously insufficient.
Although there have also been reports on the use of seeds for artificial propagation, due to the low seed germination rate and high seedling mortality, artificial propagation has little effect

Method used

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  • Regeneration culture method of saussurea involucrata
  • Regeneration culture method of saussurea involucrata
  • Regeneration culture method of saussurea involucrata

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Experimental program
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Effect test

Embodiment 1

[0081] Embodiment 1: Dynamic water culture method of Snow Lotus in Tianshan Mountains

[0082] 1.1 Germination of snow lotus seeds

[0083] Put the seeds of Saussurea tianshanensis into a 50mL centrifuge tube, add 200mL / L gibberellin (GA3), put them in a refrigerator at 4 °C, and treat them in the dark and at low temperature for 12 hours, spread the treated seeds on filter paper or a petri dish, Culture under light, culture conditions are as follows: light intensity is 2000-3000Lx, temperature is 25 ℃, humidity is 50-60%, culture 3-5 days can germinate, obtain the seed that the experimental group germinates ( figure 1 ).

[0084] Put the seeds of Snow Lotus Tianshan into a 50mL centrifuge tube, place them in a refrigerator at 4°C, and treat them in the dark and at low temperature for 12 hours. Spread the treated seeds on filter paper or a petri dish, and cultivate them under light. The culture conditions are as follows: the light intensity is 2000 -3000 Lx, the temperature i...

Embodiment 2

[0092] Embodiment 2: the regenerative culture method of Snow Lotus in Tianshan Mountains

[0093] 2.1 Disinfection of explants

[0094] Get the explant (blade, petiole, root) of the 2-month-old Saussurea tianshanensis that embodiment 1 obtains as material, carry out disinfection. The disinfection process is as follows: rinse with tap water for 1 hour, rinse with sterile water for 3-5 times in an ultra-clean workbench, soak in 10% NaClO for 5-10 minutes, disinfect with 75% alcohol for 30 seconds, and wash with sterile water.

[0095] 2.2 Callus induction

[0096] First cut the explants to 1cm with scissors 2 size( Figure 5 ), and then placed on callus induction medium for callus induction culture.

[0097] The culture conditions for callus induction are as follows: culture in the dark, the temperature is 25° C., and the humidity is 50-60%.

[0098] Every liter of callus induction medium consists of MS powder 3.32g, sucrose 30g, NAA 1.5mg, 6-BA 1mg, phytogel 4g and 1L wate...

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Abstract

The invention discloses a regeneration culture method of saussurea involucrata. The regeneration culture method of the saussurea involucrata comprises a new culture mode of the saussurea involucrata, namely development of a water culture system and optimization of a tissue culture and rapid propagation system of the saussurea involucrata, and the two are organically combined, so that the problem of shortage of wild resources of the saussurea involucrata is effectively solved, and the limitation of flat ground growth of the saussurea involucrata is broken through; the tissue culture and rapid propagation time is shortened to the maximum extent, and manpower, material resources and financial resources are saved. And an important basis is provided for resource and protection research of the saussurea involucrate.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for regenerating and cultivating Saussurea tianshanensis, in particular to a method for dynamic hydroculture of Saussurea tianshanensis in a low-altitude and flat environment and a method for improving tissue culture and rapid propagation of snow lotus tianshanensis. Background technique [0002] Saus-surea involucrata (Kar. et Kir.) Sch.-Bip.) is a perennial alpine herbaceous plant belonging to the family Asteraceae. Since ancient times, Tianshan Snow Lotus has been a traditional precious Chinese medicinal material. It is recorded in the "Chinese Pharmacopoeia" that Tianshan Snow Lotus is warm in nature and slightly bitter. For the treatment of wind-cold-damp arthralgia, abdominal pain, irregular menstruation, etc. The main chemical components of snow lotus are alkaloids, flavonoids, and phenylpropanoids, which endow snow lotus with extremely high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01C1/00
CPCA01H4/001A01H4/008A01G31/00A01C1/00
Inventor 付春祥吴振映曹英萍孙震刘雨辰
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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