Artemisia lindleyana alcohol extract as well as extraction method and application thereof
An extraction method, a technology of artemisinin, applied in the field of ethanol extract of Artemisia annua Central Asia and its extraction
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Embodiment 1
[0044] Crush the above-ground part of Artemisia chinensis, pass through a 40-mesh sieve, collect the powder under the sieve, add it to 85% ethanol aqueous solution, and the ratio of solid to liquid is 1:10 (g:mL). After it was fully mixed, it was placed in a water bath at 60°C, and extracted at a constant temperature for 3 times, 2 hours each time. After the extraction, the solid and liquid were separated, and the supernatants were combined, concentrated by rotary evaporation until there was no ethanol smell, and freeze-dried to obtain 85% ethanol extract of Artemisia chinensis. Dissolve the 85% alcoholic extract of Artemisia subtilis in distilled water, adjust it to a concentration of 60mg / mL alcoholic extract solution, pass through the AB-8 macroporous resin column, and successively dissolve with water, 30%, 50%, 70%, and 85% ethanol Gradient elution of aqueous solution, absolute ethanol, etc., each gradient eluted with 4 column volumes, among which the components eluted wit...
Embodiment 2
[0047] Crush the above-ground part of Artemisia chinensis, pass through a 50-mesh sieve, collect the powder under the sieve, add it to 80% ethanol aqueous solution, and the ratio of solid to liquid is 1:12 (g:mL). After it was fully mixed, it was placed in a 65°C water bath, and extracted twice at a constant temperature, each time for 3 hours. After the extraction, the solid and liquid were separated, and the supernatants were combined, concentrated by rotary evaporation until there was no ethanol smell, and freeze-dried to obtain 80% ethanol extract of Artemisia chinensis. Dissolve the 80% alcoholic extract of Artemisia chinensis with distilled water, adjust it to a concentration of 60 mg / mL alcoholic extract solution, pass through the AB-8 macroporous resin column, and successively use water, the volume concentration is 30%, 50%, 70%, 85% ethanol aqueous solution and absolute ethanol gradient elution, each gradient elution has 3 column volumes, after screening, the optimal c...
Embodiment 3
[0050] Crush the above-ground part of Artemisia annua, pass it through a 30-mesh sieve, collect the powder under the sieve, add it to 90% ethanol aqueous solution, and the ratio of solid to liquid is 1:8 (g:mL). After it was fully mixed, it was placed in a water bath at 55°C, and extracted at a constant temperature for 3 times, each time for 3 hours. After the extraction, the solid and liquid were separated, and the supernatants were combined, concentrated by rotary evaporation until there was no ethanol smell, and freeze-dried to obtain 90% ethanol extract of Artemisia chinensis. Dissolve the 90% alcoholic extract of Artemisia subtilis in distilled water, adjust it to a concentration of 60mg / mL alcoholic extract solution, pass through the AB-8 macroporous resin column, and successively add water, 30%, 50%, 70%, and 85% ethanol aqueous solution , anhydrous ethanol and other gradient elutions, each gradient elution has 4 column volumes, and the components eluted with 30% ethano...
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