Persimmon leaf flavone extract and preparation method thereof

A technology for persimmon leaf flavonoids and extracts, which is applied to the field of persimmon leaf flavonoids extract and its preparation, can solve the problems of thick extract, poor water solubility of the extract, time-consuming drying and the like, and achieves high flavonoid content and reduced safety. Risk and environmental cost, time-consuming reduction effect

Active Publication Date: 2022-03-25
广州思沛医药科技股份有限公司
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  • Claims
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Problems solved by technology

[0005]2015 Chinese Pharmacopoeia (Part One) has three main aspects about the preparation method of persimmon leaf flavonoid extract: 1. The organic solvent ethyl acetate will be used in the preparation method Esters, the risk of explosion in industrial production and the environmental protection cost of hazardous waste disposal in the later stage have increased the actual production burden of the enterprise; 2. The extract contains a large number of non-flavonoid long-chain macromolecules, resulting in a very thick extract. Drying is very time-consuming; 3. The water solubility of the extract is poor, and organic solvents remain, which increases the difficulty of preparation and increases the cost of testing
(2), extract by boiling water, add alkaline substances to help dissolve, filter, adsorb resin chromatography on the filtrate, then elute, and collect the eluate. What this method obtains is also a crude extract, although it has the function of lowering blood pressure and promoting The activity of plant growth, but there are still many impurities, which cannot be used as medicinal ingredients for human body
This method will use the organic solvent ethyl acetate, and the process is relatively cumbersome, and the extraction effect is still not ideal

Method used

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  • Persimmon leaf flavone extract and preparation method thereof
  • Persimmon leaf flavone extract and preparation method thereof

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Embodiment 1

[0034] The preparation method of embodiment 1 persimmon leaf flavone extract

[0035] The preparation method of the persimmon leaf flavonoids extract of the present embodiment comprises the following steps:

[0036] (1) Take 4 kg of dried persimmon leaves and cut them into pieces, put them into a non-woven bag, add 40 kg of water, adjust the pH to 7.5 with sodium hydroxide, soak overnight (room temperature, more than 8 hours);

[0037] (2), decoct the soaking solution for 2 hours, filter for later use;

[0038] (3) Add 40kg of water to the persimmon leaves, adjust the pH to 7.5 with sodium hydroxide, decoct for 1 hour again, and filter;

[0039] (4), merge twice filtrate, under 60 ℃ vacuum (-0.1Mpa) concentrate filtrate to relative density 1.12~1.15 (60 ℃, utilize the density meter to measure the relative density of concentrated solution), get initial concentrated solution (the primary concentrated solution Store at 4°C for later use), with a total weight of about 150g;

[...

Embodiment 2

[0048] The preparation method of embodiment 2 persimmon leaf flavone extract

[0049] The preparation method of the persimmon leaf flavonoids extract of the present embodiment comprises the following steps:

[0050] (1) Take 2 kg of dried persimmon leaves and cut them into pieces, put them into a non-woven bag, add 24 kg of water, adjust the pH to 7.8 with sodium hydroxide, soak overnight (room temperature, more than 8 hours);

[0051] (2), decoct the soaking solution for 2 hours, filter for later use;

[0052] (3) Add 24kg of water to the persimmon leaves, adjust the pH to 7.8 with sodium hydroxide, decoct for 1 hour again, and filter;

[0053] (4), merge twice filtrate, under 60 ℃ vacuum (-0.1Mpa) concentrate filtrate to relative density 1.12~1.15 (60 ℃, utilize the density meter to measure the relative density of concentrated solution), get initial concentrated solution (the primary concentrated solution Store at 4°C for later use), weighing about 75g;

[0054] (5), take...

Embodiment 3

[0060] The preparation method of embodiment 3 persimmon leaf flavone extract

[0061] The preparation method of the persimmon leaf flavonoids extract of the present embodiment comprises the following steps:

[0062] (1) Take 2 kg of dried persimmon leaves, cut them into pieces, put them into a non-woven bag, add 16 kg of water, adjust the pH to 8.0 with sodium hydroxide, soak overnight (room temperature, more than 8 hours);

[0063] (2), decoct the soaking solution for 3 hours, filter for later use;

[0064] (3), add 16kg of water to the persimmon leaves, adjust the pH to 8.0 with sodium hydroxide, decoct for 2 hours again, and filter;

[0065] (4), combine the two filtrates, concentrate the filtrate to a relative density of 1.12 to 1.15 (at 60°C, use a density meter to measure the relative density of the concentrate), and obtain the primary concentrate (preserve the primary concentrate at 4°C for future use), weighing about 75g ;

[0066] (5), take the initial concentrate ...

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Abstract

The invention discloses a preparation method of a persimmon leaf flavone extract, which comprises the following steps: soaking persimmon leaves in water with the pH value of 7.5-8.0, decocting, and taking filtrate; concentrating, loading on a macroporous resin column, eluting with water, combining eluents with the total flavonoid content of more than or equal to 0.6%, and concentrating to obtain a persimmon leaf water-soluble extract; combining the eluents with the total flavonoid content of less than 0.6%, concentrating, loading on a macroporous resin column, eluting with alcohol, collecting the alcohol eluent, and concentrating to obtain a persimmon leaf alcohol-soluble extract; and combining the persimmon leaf water-soluble extract and the persimmon leaf alcohol-soluble extract, concentrating and drying to obtain the persimmon leaf extract. The persimmon leaf flavone extract obtained through the preparation method is high in flavonoid substance content, the time consumed in the preparation process is greatly shortened, an organic solvent ethyl acetate does not need to be used, and the safety risk and the environmental protection cost of actual production of enterprises are reduced.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients of medicines, and more specifically, the invention relates to a persimmon leaf flavonoid extract and a preparation method thereof. Background technique [0002] Persimmon leaves are bitter in taste and cold in nature. They have the effects of lowering Qi and relieving asthma, quenching thirst and promoting body fluid, and treating sores, and have a wide range of pharmacological effects. The main active ingredient in persimmon leaves is flavonoids. Domestic and foreign studies have shown that persimmon leaf flavonoids have the functions of lowering blood pressure, inhibiting bacteria, lowering blood lipids, lowering cholesterol, stopping bleeding, promoting blood circulation and removing blood stasis, inhibiting tumor growth, and promoting plant growth. The flavonoid compounds of persimmon leaves that have been found so far include astragaloside, isoquinetin, kaempferol, querc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/44A61K127/00
CPCA61K36/44A61K2236/331A61K2236/51A61K2236/55
Inventor 王严春于志瀛贺肇东吴华赟
Owner 广州思沛医药科技股份有限公司
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