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Freezing preservation solution for semen of lota lota as well as preparation method and application of freezing preservation solution

A technology for cryopreserving liquid and semen, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, and can solve the problems of complex composition, unstable effect, and difficulty in cryopreservation of Irtysh cod sperm.

Pending Publication Date: 2022-04-05
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Practical operations have proved that these three formulas are not only complex in composition, but also unstable in effect, and it is difficult to successfully achieve cryopreservation of Irtysh River burbot sperm

Method used

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  • Freezing preservation solution for semen of lota lota as well as preparation method and application of freezing preservation solution
  • Freezing preservation solution for semen of lota lota as well as preparation method and application of freezing preservation solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of burbot semen cryopreservation liquid, the burbot semen cryopreservation liquid comprises the components of following concentration:

[0027] 0.3mol / L glucose, 10% methanol, 10% egg yolk and KCl 1.79g / L.

[0028] The preparation method of burbot semen cryopreservation solution comprises the steps:

[0029] 1), weigh 10 grams of methanol, 5.4 grams of glucose, 10 grams of egg yolk and 0.179g of KCl;

[0030] 2) Dissolve the mixture in step 1) into 70ml of purified water:

[0031] 3) After stirring the mixture in step 2), add 30ml of pure water, and finally make it to 100ml.

Embodiment 2

[0033] A kind of burbot semen cryopreservation liquid, the burbot semen cryopreservation liquid comprises the components of following concentration:

[0034] 0.3mol / L glucose, 10% methanol and 10% egg yolk.

[0035] The preparation method of burbot semen cryopreservation solution comprises the steps:

[0036] 1), weigh 10 grams of methanol, 5.4 grams of glucose and 10 grams of egg yolk;

[0037] 2) Dissolve the mixture in step 1) into 70ml of purified water:

[0038] 3) After stirring the mixture in step 2), add 30ml of pure water, and finally make it to 100ml.

Embodiment 3

[0040] The test materials of the effect of semen cryopreservation solution on the activation ratio of burbot sperm are as follows:

[0041] 1), test material

[0042] Choice of broodstock:

[0043] The fish used in the experiment was wild burbot in the Irtysh River, and healthy and mature male fish were selected before use;

[0044] 2), test location

[0045] The water temperature for breeding broodstock is 2°C, and the breeding site takes necessary measures to avoid light, keep relatively dark light, and try to avoid the killing of sperm by light;

[0046] 3), test reagents

[0047] Glucose, methanol, NaCl, KCl, CaCl2·2H2O, MgSO4·7H2O, Hepes and egg yolk.

[0048] The test method for the effect of semen cryopreservation solution on the activation ratio of burbot sperm is as follows:

[0049] S1. Preparation of semen cryopreservation solution

[0050] 1) Preparation of No. 1 semen cryopreservation solution: weigh 0.584g of NaCl, 0.0015g of KCl, 0.011g of CaCl2 2H2O, 0.01...

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Abstract

The invention discloses lota lota seminal fluid cryopreservation liquid as well as a preparation method and application thereof. Seminal fluid is obtained from mature male fish by an abdomen extrusion method, three kinds of seminal fluid cryopreservation liquid with different formulas are respectively mixed with the seminal fluid according to the proportion of 3: 1, the mixture is put into a 0.5 mL straw, the straw is cooled for 10 minutes on a floating frame 2cm away from the liquid nitrogen surface in a foam box, and the straw is preserved in a liquid nitrogen tank. And after thawing (40 DEG C, 10s), observing and recording the sperm activation proportion and sperm survival time. The sperm activation proportion and survival time of the semen cryopreservation liquid II and the semen cryopreservation liquid III are obviously higher than those of the semen cryopreservation liquid I, and the sperm activation proportion and survival time of the semen cryopreservation liquid II are higher than those of the semen cryopreservation liquid III but do not reach the obvious difference level. In the three semen cryopreservation solutions used in the research, the activation proportion and the sperm survival time of the frozen semen obtained by the No. II with simple components are highest, and the semen cryopreservation solution can be used for cryopreservation of the lota lota semen.

Description

technical field [0001] The invention relates to the technical field of burbot breeding, in particular to a burbot semen cryopreservation solution, a preparation method and an application thereof. Background technique [0002] In taxonomy, burbot belongs to the order Codidae, Codidae, and burbot. It is a fierce carnivorous fish and an important large-scale cold-water economic fish. It is widely distributed in Eurasia and North America north of 45° north latitude. Inland waters are only distributed in the upper reaches of the Irtysh River, Heilongjiang River and Yalu River in China. The meat of burbot is delicious and delicate, with high nutritional value, and its fat liver is even more delicious. Due to the unique value and development potential of burbot, scholars at home and abroad have carried out certain research on the biological characteristics, artificial reproduction, breeding and development of burbot, and have gone deep into genomics research. However, recently, d...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张俊杰李胜忠马允王位莹
Owner XINJIANG AGRI UNIV
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