Novel immunoturbidimetric kit for detecting procalcitonin

A technology of procalcitonin and immunoturbidimetry, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of false positives, low monoclonal antibody reactivity, and low absorbance, so as to reduce the difference between batches and ensure reliable Reliability, the effect of eliminating interference

Inactive Publication Date: 2022-04-05
安徽环球基因科技有限公司
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Problems solved by technology

[0004] Existing antibodies are polyclonal antibodies with large batch-to-batch variation; there are few target antibodies against epitopes, and there are mixed antibodies, resulting in low absorbance near the cut-off value and prone to false positives; currently on the market The antibodies used in the procalcitonin i

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  • Novel immunoturbidimetric kit for detecting procalcitonin
  • Novel immunoturbidimetric kit for detecting procalcitonin
  • Novel immunoturbidimetric kit for detecting procalcitonin

Examples

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Embodiment 1

[0029] The preparation of polystyrene latex microspheres coupled with mouse anti-human procalcitonin monoclonal combined antibody comprises the following steps:

[0030] The mouse anti-human procalcitonin monoclonal combined antibody was prepared by mixing monoclonal antibody PCT-124E7, monoclonal antibody PCT-11B8 and monoclonal antibody PCT-37G5 at a mass ratio of 1:1:1;

[0031] The average particle diameter of the polystyrene latex microspheres containing carboxyl groups on the surface is 100nm, and the density is 0.03mmol / g;

[0032] Mix 0.1 mL of polystyrene latex microspheres containing carboxyl groups on the surface with 0.9 mL of MES buffer solution with a pH value of 6.0 and a concentration of 0.01 mol / L, pipette evenly, and then add 0.2 mL of EDAC / NHS for activation. React in a constant temperature shaker at 25°C for 15 minutes, centrifuge at a centrifugal force of 15,000g for 30 minutes, remove the supernatant, and buffer the precipitate with 1 mL of 4-hydroxyethyl...

Embodiment 2

[0034] The preparation of polystyrene latex microspheres coupled with mouse anti-human procalcitonin monoclonal combined antibody comprises the following steps:

[0035] The mouse anti-human procalcitonin monoclonal combined antibody was prepared by mixing monoclonal antibody PCT-124E7, monoclonal antibody PCT-11B8 and monoclonal antibody PCT-37G5 at a mass ratio of 1:1:1;

[0036] The average particle diameter of the polystyrene latex microspheres containing carboxyl groups on the surface is preferably 400nm, and the density is 0.30mmol / g;

[0037] Mix 0.1 mL of polystyrene latex microspheres containing carboxyl groups on the surface with 0.9 mL of MES buffer solution with a pH value of 6.0 and a concentration of 0.01 mol / L, pipette evenly, and then add 0.2 mL of EDAC / NHS for activation. React in a constant temperature shaker at 25°C for 20 minutes, centrifuge at a centrifugal force of 15,000g for 30 minutes, remove the supernatant, and buffer the precipitate with 1 mL of 4-hyd...

Embodiment 3

[0039] The preparation of polystyrene latex microspheres coupled with mouse anti-human procalcitonin monoclonal combined antibody comprises the following steps:

[0040] The mouse anti-human procalcitonin monoclonal combined antibody was prepared by mixing monoclonal antibody PCT-124E7, monoclonal antibody PCT-11B8 and monoclonal antibody PCT-37G5 at a mass ratio of 1:1:1;

[0041] The average particle diameter of the polystyrene latex microspheres containing carboxyl groups on the surface is preferably 270nm, and the density is 0.134mmol / g;

[0042] Mix 0.1 mL of polystyrene latex microspheres containing carboxyl groups on the surface with 0.9 mL of MES buffer solution with a pH value of 6.0 and a concentration of 0.01 mol / L, pipette evenly, and then add 0.2 mL of EDAC / NHS for activation. React in a constant temperature shaker at 25°C for 20 minutes, centrifuge at a centrifugal force of 15,000g for 30 minutes, remove the supernatant, and buffer the precipitate with 1 mL of 4-...

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Abstract

The invention discloses a novel immunoturbidimetric kit for detecting procalcitonin, and belongs to the technical field of detection reagents, a CE510 sealing agent in an R2 reagent can occupy blank sites on latex microspheres to avoid the agglutination phenomenon of the latex microspheres; according to the present invention, the tea saponin with a certain concentration is added to the R1 reagent, and the tea saponin is the strong surfactant and further has the lipid dissolving effect, such that the interference of different sample detection reagents at different degrees can be eliminated, and the combination of the monoclonal antibody and the target object cannot be interfered so as to ensure the credibility of the detection result; the kit adopts the monoclonal antibody, so that the batch-to-batch difference of the kit can be reduced. A latex enhanced immunoturbidimetric assay test shows that the labeled combined monoclonal antibody used in the latex enhanced immunoturbidimetric assay kit is highly consistent with a gold standard Rogowski electrochemical luminescence detection value, and the accuracy is far higher than that of an existing kit for labeling a polyclonal antibody.

Description

technical field [0001] The invention belongs to the technical field of detection reagents, in particular to a novel immunoturbidimetric kit for detecting procalcitonin. Background technique [0002] Immunoturbidimetric kits are generally used in medical testing. The principle of latex-enhanced immunoturbidimetric method is: the target in the sample binds to the antibody on the surface of latex particles. The higher the concentration of the target in the sample, the higher the concentration of the latex surface antibody. The greater the degree of binding by it, the higher the degree of latex aggregation that can be induced. The turbidity of the reaction system is positively correlated with the concentration of the target substance in the sample. By measuring the absorbance at a specific wavelength, a calibration curve is established to calculate the concentration of the target substance . [0003] At present, the antibody coupled to the surface of latex microspheres is a pol...

Claims

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Application Information

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IPC IPC(8): G01N33/539G01N33/543G01N33/577
CPCG01N33/539G01N33/54313G01N33/577G01N2333/585
Inventor 缪连军张磊门静涛雍金贵郁春云赵杰沈健周堃唐爱阳徐庆阳
Owner 安徽环球基因科技有限公司
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