Pigeon source lactobacillus johnsonii and application thereof

A technology of Lactobacillus johnsonii derived from pigeons and Lactobacillus johnsonii, which is applied to Lactobacillus johnsonii derived from pigeons and its application fields, can solve the problems that traditional Chinese medicine cannot be directly mixed into feed, it is difficult to feed pigeons, and the cost is high, so as to achieve good development Application prospects, reducing liver displacement and body inflammatory response, and improving production performance

Active Publication Date: 2022-04-12
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, research on the prevention and control of Salmonella typhimurium from pigeons only focuses on alternative feed additives such as Chinese herbal medicines, but only 4 of the 14 reported Chinese herbal medicines have been confirmed to have obvious antibacterial effects in vitro, and there is no relevant data in vivo.
In addition, the cost of using traditional Chinese medicine to treat salmonellosis is relatively high. Since the current pigeon diet is mainly raw food, traditional Chinese medicine cannot be directly mixed into the feed, and some traditional Chinese medicine is insoluble in water, so it is not easy to feed pigeons.

Method used

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  • Pigeon source lactobacillus johnsonii and application thereof
  • Pigeon source lactobacillus johnsonii and application thereof
  • Pigeon source lactobacillus johnsonii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Isolation, identification, acquisition of embodiment 1 lactic acid bacteria strain

[0025] 1. Isolation of lactic acid bacteria strains

[0026] Collect fresh pigeon milk in the crop of 4-day-old pigeons, weigh 5g, place it in a centrifuge tube filled with 25ml sterile physiological saline, shake it well, and then absorb 0.1ml bacterial liquid into 0.9ml sterile physiological saline. In a test tube of saline, this dilution is 10 -1 , repeat the above process, and make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 of bacteria liquid. choose 10 -5 、10 -6 、10 -7 For three dilutions, 0.1ml of the bacterial solution was drawn and dropped on the above medium plate, cultured in an anaerobic tank at 37°C for 24 hours, and typical colonies were picked for 16SrDNA identification.

[0027] The seven lactic acid bacteria strains obtained through isolation and identification were numbered S011, S028, S029, S031, S034, S035, and S043, respectively. Inoculate in MRS liquid med...

Embodiment 2

[0047] The physiological characteristic of embodiment 2 pigeon source Lactobacillus johnsonii

[0048] 1. Growth curve

[0049] After the above-mentioned obtained numbering is S034 (that is, BL4026, that is, CGMCC No.21215), after the activation of the pigeon-derived Lactobacillus johnsonii strain, inoculate it in the MRS liquid medium with an inoculum size of 1%, cultivate it at 37° C., and cultivate it respectively. At 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 60, 72h, measure the OD value and take a certain amount of bacterial solution The number of colonies was measured on the plate, and the growth curve was drawn. Within 72 hours of growth, with the different culture time, the absorbance value at 600nm is as follows: image 3 shown. With the different culture time, the number of colonies such as Figure 4 shown. It can be seen from the results that the number of colonies is the largest after 30 hours of growth.

[0050] 2. Antibacterial effect ...

Embodiment 3

[0052] Application effect of embodiment 3 pigeon source Lactobacillus johnsoni in the production of meat pigeons

[0053] 1. Preparation of pigeon-derived Lactobacillus johnsonii liquid

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Abstract

The invention provides a pigeon source lactobacillus johnsonii strain, and the preservation number of the pigeon source lactobacillus johnsonii strain is CGMCC (China General Microbiological Culture Collection Center) No. 21215. The bacterial strain disclosed by the invention has excellent antibacterial performance, especially has a remarkable antibacterial effect on pigeon-derived salmonella typhimurium, and the bacterial strain and related microbial agents provided by the invention can be used for reducing inflammatory response of young pigeons, reducing liver displacement of salmonella and inhibiting reduction of weight of infected pigeons, and have good development and application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pigeon-derived Lactobacillus johnsonii and an application thereof. Background technique [0002] Pigeon meat and pigeon eggs have high nutritional value and good meat taste. With the improvement of living standards, people's demand for pigeon meat and pigeon eggs continues to increase, and domestic pigeon breeding has developed rapidly. However, with the expansion of breeding scale, the occurrence of pigeon diseases is also more complicated. Among them, pigeon salmonellosis is the main bacterial disease, mainly caused by Salmonella typhimurium. It can not only spread horizontally, but also vertically spread through breeding pigeons feeding squabs. Cause huge economic loss to pigeon industry. Due to the large number of serotypes, there is currently no commercialized vaccine to prevent pigeon salmonellosis, and many farmers rely on the use of antibiotics to prevent and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/70A23K10/18A61K35/747A61P31/04C12R1/225
CPCY02A50/30
Inventor 邵玉新王铮
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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